TITLE

In vitro maturation system for individual culture of bovine oocytes using micro-volume multi-well plate

AUTHOR(S)
Masashi Nagano; Sung-Sik Kang; Keisuke Koyama; Weiping Huang; Yojiro Yanagawa; Yoshiyuki Takahashi
PUB. DATE
November 2013
SOURCE
Japanese Journal of Veterinary Research;Nov2013, Vol. 61 Issue 4, p149
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
As a preliminary study for the development of individual in vitro maturation (IVM) culture of bovine oocytes, a multi-well (MW) plate was used. Maturation, fertilization and development to blastocysts were examined and compared with those of IVM oocytes cultured in 50-µl droplets in groups and in 10-µl droplets individually. The maturation rates were similar in all experimental groups. Normal fertilization rates in MW and 50-µl droplets were similar, but lower in 10-µl droplets (p < 0.01). The blastocyst rate in 10-µl droplets tended to be lower than those in MW (p = 0.15) and 50-µl droplets (p = 0.19). These results indicate that an IVM system using MW supports the acquisition of developmental competence by bovine oocytes the same as conventional group IVM culture.
ACCESSION #
92964965

 

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