TITLE

miR-200b Suppresses Cell Growth, Migration and Invasion by Targeting Notch1 in Nasopharyngeal Carcinoma

AUTHOR(S)
Yang, Xu; Ni, Weichun; Lei, Ke
PUB. DATE
December 2013
SOURCE
Cellular Physiology & Biochemistry (Karger AG);Dec2013, Vol. 32 Issue 5, p1288
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background/Aims: MicroRNAs (miRNAs) are a class of small noncoding RNA molecules that play important roles in carcinogenesis and tumor progression. We investigated the roles and mechanisms of miR-200b in human nasopharyngeal carcinoma (NPC). Methods: We used quantitative real-time polymerase chain reaction (qRT-PCR) analyses to measure levels of miR-200b and Notch1 in NPC specimens and cell lines. Human NPC cell lines stably expressing miR-200b or control were used to analyze the tumour-suppressive effect of miR-200b. Luciferase reporter assays were used to determine the association between miR-200b and the Notch1 3' untranslated region. Results: We found that miR-200b is significantly downregulated in NPC tissues and cell lines. Gain-of-function and loss-of-function studies demonstrated that miR-200b suppresses NPC cell growth, migration and invasion in vitro. Importantly, overexpression of miR-200b effectively repressed tumor growth in nude mouse models. Integrated analysis identified Notch1 as a direct and functional target of miR-200b. Overexpression of Notch1 reversed the inhibitory effect of miR-200b on NPC cell growth and invasion. Conclusion: These results indicate that miR-200b exerts tumor-suppressive effects in NPC carcinogenesis through the suppression of Notch1 expression and suggest a therapeutic application of miR-200b in NPC. © 2013 S. Karger AG, Basel
ACCESSION #
92933727

 

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