TITLE

Morphological, morphometrical and molecular (CO1 and ITS) analysis of the rotifer Asplanchna brightwellii from selected freshwater bodies in Central Mexico

AUTHOR(S)
Jiménez-Contreras, Jorge; Sarma, S. S. S.; Piedra-lbarra, Elias; Calderón-Torres, Marissa; Nandini, S.
PUB. DATE
November 2013
SOURCE
Journal of Environmental Biology;Nov2013, Vol. 34 Issue 6, p1039
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
We evaluated different strains of the rotifer Asplanchna brightweiiii collected from central Mexico using morphology, morphometry and molecular tools (COI and ITS). Three distinct clonal populations from each of the 3 regions (Mexico City, State of Mexico and State of Guerrero) were established under laboratory conditions. For a given waterbody, morphometric comparisons within the populations of A. brightweiiii showed almost stable measurements of trophi and with no statistically significant differences among them (p>0.05). However, asplanchnid body length and width as well as the cyst diametervaried significantly depending on the waterbody from which A. brightweiiii was collected. The smallest adults (about 700 pm) were from Valerio Trujano lake (Guerrero State) samples while the largest were from Xochimilco lake. Similar tendencies were reflected in the diameter of resting eggs. In addition, morphologically the cysts of A. brightweiiii from the three waterbodies showed slightly different pattern. The number of globular structures on the surface of cysts was smaller for Valerio Trujano strain, while these were larger and less numerous for both Xochimilco and Zumpango strains. The ITS region tree displayed two groups Xochimilco and Valerio Trujano -Zumpango, this analysis did not reflect the morphological grouping; on the contrary the COI gene tree separated the populations according to morphological clusters and location (Xochimilco, Valerio Trujano and Zumpango lakes). When the tree was built using the combination of both ITS and COI sequences, the phylogenetic relationships observed on COI gene were consistent; but showed differences with the relationships observed on ITS region tree (only two groups).
ACCESSION #
91986128

 

Related Articles

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics