Comparative Antibacterial Activity of Different Plant Extracts in Relation to their Bioactive Molecules, as Determined by LC-MS Analysis

POP, Cosmin; VODNAR, Dan; RANGA, Floricuţa; SOCACIU, Carmen
January 2013
Bulletin of the University of Agricultural Sciences & Veterinary;2013, Vol. 70 Issue 1, p86
Academic Journal
The research of plant responses against antibiotics and chemical disinfectants is increasingly motivated by natural antimicrobial agents. We investigated hydrophilic extracts of leaves of Persian walnut (Juglans regia) and European Mistletoe (Viscum album) and White willow bark (Salix alba) which were characterized by their content in phenolic derivatives, using a high-throughput technique, HPLC-DAD. The extracts were obtained using 10% dry plant and hot acidulated water (around 85°C) and kept for 48 hours then diluted successively (from 2 to 9 times). The antimicrobial activities were tested semi-quantitative ly on agar plates inoculated with E. Coli, Staphylococcus aureus, Listeria monocytogenes, Bacillus cereus and Salmonella enteritis. The inhibition zones were measured after 24 hours of incubation. Using a liquid nutrient broth growth medium, a quantitative evaluation of antimicrobial effect was done. There were noticed to be significant the growth rates in the first 10 hours, the best inhibitory results being given by Salix alba extract which inhibited all bacteria. Meanwhile, walnut and mistletoe extracts showed different inhibition rates, depending on the bacteria and the extract dilution. The extracts' composition as determined by HPLC was compared with the antimicrobial activity, suggesting that willow extract is the richest in phenolics, especially in phenolic acids (salicylic acid derivatives). This study suggest that Salix alba bark and Juglans regia are available and efficient sources of natural antimicrobials and suggests the possibility of using their hydrophilic extracts as bio-disinfectants in food industry.


Related Articles

  • Interventions could decrease broad-spectrum antibiotic use.  // Infectious Disease News;Mar2014, Vol. 27 Issue 3, p54 

    The article discusses research on the effects of interventions on antibiotics use, referencing a study by Louise Elaine Vaz and colleagues published in a 2014 issue of the journal "Pediatrics."

  • Phytochemical Screening, Antioxidant, Antimicrobial and Toxicity Activities of Polar and Non-Polar Extracts of Albizia zygia (DC) Stem-Bark. Oloyede, Ganiyat K.; Ogunlade, Adeniyi O. // Annual Review & Research in Biology;Oct-Dec2013, Vol. 3 Issue 4, p1020 

    Aims: To determine the secondary plant metabolites present in Albizia zygia (DC) stem- bark and investigate its antioxidant, antimicrobial and toxicity activities. Study Design: Methanol (polar) and hexane (non-polar) were used to extract the dried stem bark of A. zygia and biological activities...

  • Biological activities of extract prepared from Sorbus sibirica fruit. Jie Wei; Jia Shi; Jun Gao; Zhiquan Zhou; Jungang Fan // Journal of Chemical & Pharmaceutical Research;2014, Vol. 6 Issue 5, p1369 

    In this paper, we investigated the biological activities of extract prepared from Sorbus. sibirica fruits using various in vitro assays. It possessed antibacterial and antifungal activities as determined by growth inhibition assay. Among the eight different microorganisms tested, Staphylococcus...

  • Antimicrobial activity of Yemeni myrrh mouthwash. Almekhlafi, Sadik; Thabit, Anes A. M.; Alwossabi, Ameen M. I.; Awadth, Nasser; Thabet, Abdulbaqi A. M.; Algaadari, Zaid // Journal of Chemical & Pharmaceutical Research;2014, Vol. 6 Issue 5, p1006 

    This study aimed to prepare pharmaceutical formulations of mouthwashes, containing extracted Yemeni myrrh as a single active constituent, and testing their quality criteria and antimicrobial activity on common pathogens of the oral cavity. In order to determine the best extracting solvent for...

  • IN VITRO EVALUATION OF PHYTOCHEMICAL, ANTIMICROBIAL AND ANTIOXIDANT ACTIVITY OF CALYCES OF ROSELLE (HIBISCUS SABDARIFFA L.). Das, Suman // International Journal of Pharmaceutical Sciences & Research;2014, Vol. 5 Issue 8, p3364 

    Diversity of plants has attracted a great deal of research interest in search of natural antioxidants and antimicrobials. Many natural substances having anti-oxidant and anti-microbial properties have been used in health foods for medicinal and preservative purposes Fleshy calyces of Hibiscus...

  • EVALUATION OF ANTIOXIDANT POTENTIAL AND ANTIBACTERIAL ACTIVITY OF CRUDE EXTRACTS CATHARANTHUS ROSEUS. Ethalsha, P. // International Journal of Pharmaceutical Sciences & Research;2014, Vol. 5 Issue 8, p3490 

    The aim of the present study was to determine the antibacterial activity of crude extracts from root parts of Catharanthus roseus against several bacterial species of clinical significance. Root part of C. roseus was extracted in appropriate solvent followed by evaluation of antibacterial...

  • Total phenolic and flavonoids content, and in vitro antioxidant and antimicrobial activity of ethyl acetate and butanol extract from Senecio delphinifolius Vahl. Arab, Yasmine; Zellagui, Amar; Boutellaa, Saber; Mesbah, Khaled; Gherraf, Noureddine // Der Pharma Chemica;2014, Vol. 6 Issue 4, p522 

    The present work focuses essentially on the total phenolic and flavonoidscontents, and in vitro antioxidant and antimicrobial activity of two fractions: ethyl acetate and n-butanol extracts of an Algerian endemic plant: Senecio delphinifolius. The total phenolic content was determined using...

  • Antimicrobial activity of Phellinus and Ganoderma samples against human pathogenic organisms. Sonawane, Hiralal; Gauri, Bapat; Shekhar, Bhosle; Ghole, V. S. // Journal of Pharmacy Research;Jul2014, Vol. 8 Issue 7, p1008 

    Medicinal mushroom contains the bioactive compound like polysaccharides, terpens, proteins having bioactivity. Phellinus and Ganoderma has been used effectively in various diseases including improving blood circulation, enhancing detoxication and hepatoprotection, cobmbating allergy, arthritis,...

  • In vitro Antioxidant and Antimicrobial Activities of Crude Extracts of Trigonella foenum-graecum Seeds. B. A. TEJASWINI; SIREESHA, CH.; KALADHAR, D. S. V. G. K.; D. G. RAO; SATYANARAYANA, K. V. V. V.; SUREKHA, CH. // Asian Journal of Chemistry;2012, Vol. 24 Issue 11, p5019 

    Trigonella foenum-graecum (fenugreek) is an annual herb that belongs to the family Leguminosae. Seeds of Trigonella are used as antidiabetic, inhibit cholesterol absorption (hyper cholesterolemia), antiatherosclerotic, mild antiseptic and acts as an emollient. In the present study, Trigonella...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics