The Influence of the Moment of Sampling of Potato Meristem over the In Vitro Regeneration and Differentiation Capacity

January 2013
Bulletin of the University of Agricultural Sciences & Veterinary;2013, Vol. 70 Issue 1, p104
Academic Journal
Species of economic interest, the potato is investigated in vitro in order to obtain a suitable material, reaction analysis of local varieties, or in vitro rooting. The reaction of the meristem of Solanum tuberosum L. was watched, "Christian" variety (a native variety originated from INCDCSZ Brasov) at in vitro depending on the moment of meristem tissue sampling. The sampling times: in winter in the period of rest, by forcing them to hot and dark (December); in spring at the beginning of the growing season (March), and in autumn after harvesting and storage, also through forcing the tubers to hot and dark (October). We consider that the optimal sampling period of the potato meristem is in spring (March), after living stems from rest, and the best variants are the one with Zeatin (C3) and the one with gibberellin (C6).


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