Quantitative Determination of Vitamin C in Honey and Bee Pollen Using HPLC-DAD Method

BONTA, Victoriţa; MĂRGHITAS, Liviu Al.; BOBIS, Otilia; MĂRGĂOAN, Rodica; BĂRNUłIU, Lavinia; DEZMIREAN, Daniel S.
January 2013
Bulletin of the University of Agricultural Sciences & Veterinary;2013, Vol. 70 Issue 1, p31
Academic Journal
Methods for determination of vitamin C from two used matrices -honey and bee pollen- by liquid chromatography coupled with UV-Vis diode array detector have being established. Separation was performed on a C18 column, using an isocratic 1% (v/v) formic acid in water as mobile phase. Meta-phosphoric acid was used as extraction solvent, each matrix following different sample preparation steps. The proposed methods were applied after their validations on the analysis of 50 honey and 25 bee pollen samples from Romania with different botanical origin and harvest years. Vitamin C concentration found in fresh bee pollen samples was significantly higher than the content in samples from previous years. Vitamin C may be used as important freshness indicator. In honey vitamin C content is not entirely dependent of storage period, but also by botanical origin of the samples. The developed method proved to be sensitive, accurate and without interfering compounds.


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