Quantitative Determination of Vitamin C in Honey and Bee Pollen Using HPLC-DAD Method

BONTA, Victoriţa; MĂRGHITAS, Liviu Al.; BOBIS, Otilia; MĂRGĂOAN, Rodica; BĂRNUłIU, Lavinia; DEZMIREAN, Daniel S.
January 2013
Bulletin of the University of Agricultural Sciences & Veterinary;2013, Vol. 70 Issue 1, p31
Academic Journal
Methods for determination of vitamin C from two used matrices -honey and bee pollen- by liquid chromatography coupled with UV-Vis diode array detector have being established. Separation was performed on a C18 column, using an isocratic 1% (v/v) formic acid in water as mobile phase. Meta-phosphoric acid was used as extraction solvent, each matrix following different sample preparation steps. The proposed methods were applied after their validations on the analysis of 50 honey and 25 bee pollen samples from Romania with different botanical origin and harvest years. Vitamin C concentration found in fresh bee pollen samples was significantly higher than the content in samples from previous years. Vitamin C may be used as important freshness indicator. In honey vitamin C content is not entirely dependent of storage period, but also by botanical origin of the samples. The developed method proved to be sensitive, accurate and without interfering compounds.


Related Articles

  • The Latest Buzz on Products of the Hive. Adderly, M.H.A., Brenda // Better Nutrition;Aug99, Vol. 61 Issue 8, p42 

    Focuses on healthful bee-related products. Use of honey as an effective topical antibiotic; Anti-bacterial properties of bee pollen; Stimulation of the adrenal glands by royal jelly; Propolis as an effective treatment for tuberculosis, colitis and ulcers.

  • TWO DIFFERENT METHODS USED IN DISTINGUISHING PINE HONEY FROM FLORAL HONEY. Özkök, Aslı; Çıngı, Hülya // Mellifera;2010, Vol. 10 Issue 20, p14 

    From 10 administrative districts (Centre, Milas, Ortaca, Köyceğiz, Marmaris, Fethiye, Yatağan, Bodrum, Via and Datça) of Muğla province to the west of Turkey 73 honey samples were collected in October and November in the period of 2004 - 2006. Collected honey samples were analyzed...

  • Buzzing With Nutrition. Campbell, Rosemarie // Natural Life;Nov/Dec2004, Issue 100, p14 

    Discusses the nutritional aspects of honey. Offer of fructose, glucose, water and other sugars, enzymes, vitamins, minerals and amino acids; Therapeutic powers of honey; Bug-killing properties of honey; Other products produced by bees, such as royal jelly, propolis and bee pollen; Kinds of honey...

  • Cuphea Plants far Bees. Krochmal, Connie // Bee Culture;May2014, Vol. 142 Issue 5, p88 

    The article offers information on growing cuphea plants as they help bees collect nectar and pollen from them. It mentions the technique of growing cuphea as well as offers information on the oil rich seeds of the plant that can be added to bee feed. It also describes different types of cuphea...

  • WILL YOU BEE MINE? Meyerowitz, Steve "Sproutman" // Better Nutrition;May2000, Vol. 62 Issue 5, p54 

    Focuses on the health aspects of the four main products of the hive, namely bee pollen, propolis, honey and royal jelly. Description of the products; Processes by which the products are produced in the hive; Therapeutic uses of the products; Specific diseases cured and prevented by each...

  • Can Bee Pollen Benefit Health? Mirkin, Gabe // JAMA: Journal of the American Medical Association;10/6/89, Vol. 262 Issue 13, p1854 

    Explains the action and benefit of bee pollen as a dietary supplement. Composition of bee pollen; Claims of lay publications and entrepreneurs on the benefits of bee pollen; Diseases resulting from allergy from specific pollens.

  • Physical-Chemical Composition of Fresh Bee Pollen from Transylvania. MĂRGĂOAN, Rodica; MĂRGHITAŞ, Liviu Al.; DEZMIREAN, Daniel S.; BOBIŞ, Otilia; M.ĂMIHAI, Cristina // Bulletin of the University of Agricultural Sciences & Veterinary;2012, Vol. 69 Issue 1/2, p351 

    Sixteen samples of fresh bee pollen were collected from different regions in Transylvania and analyzed regarding their moisture, lipids, proteins, ash and major carbohydrates. The obtained results showed a variability of the moisture from 17.59 to 29.55%, proteins 16.27 to 26.50%, ash from 1.75...

  • CC POLLEN - Committed To Producing High Quality Honey Bee Products.  // Bee Culture;Oct2009, Vol. 137 Issue 10, p26 

    The article profiles CC Pollen Co. in Phoenix, Arizona. It notes the company's focus on health food products globally, producing pollen, propolis, royal jelly and other hive supplemental products for humans and animals. It explores how the CC Pollen Co. started headed by Royden Brown, who was a...

  • health from the honeybee.  // Total Health;Aug/Sep2007, Vol. 29 Issue 3, p42 

    The article discusses information on the nutritional properties of bee pollen. Bee pollen is recognized as an immune system enhancer for its ability to strengthen the body against viral infections. It is effective in relieving fatigue, improving concentration, the treatment of asthma and of...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics