TITLE

DNA marker s linked to Pgal, an adzuki bean gene that confers resistance to Cadophora gregata race 1

AUTHOR(S)
Takako Suzuki; Takamitsu Yoshii; Shohei Fujita; Hisanori Shimada; Toru Takeuchi; Norio Kondo
PUB. DATE
September 2013
SOURCE
Breeding Science;Sep2013, Vol. 63 Issue 3, p353
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Brown stem rot (BSR) caused by Cadophora gregata f. sp. adzukicola (syn. Phialophora gregata) is a seri-ous soilborne disease of adzuki bean ( Vigna angularis) in Japan. Cultivation of resistant cultivars is the most effective disease control method, therefore the selection of resistant lines is a priority for breeders. BSR-resistant adzuki bean lines have been screened in pathogen-infected fields. However, field selection using the pathogen and artificial inoculation methods is time-consuming and labor-intensive. In the present study, we used 105 F3 lines derived from a cross between a BSR-resistant cultivar 'Syumari' and a susceptible cultivar 'Buchishoryukei-1' for BSR inoculation tests. Amplified fragment-length polymorphism (AFLP) analyses with 1024 primer sets revealed that six fragments were polymorphic between resistance and susceptible bulked groups. Five DNA markers (Pg77, Pgl 18, Pgl38, Pgl39 and Pgl26) were developed from the nucle-otide sequences of polymorphic AFLP markers and their flanking regions. Pgl 18, which was derived from E-ACT/M-ACT-118, was tightly linked to the resistance gene Pgal and was converted into a codominant marker for its easier use in marker-assisted selection for adzuki bean BSR resistance. Finally, the applicabil-ity of the developed markers for BSR resistance was tested on 32 adzuki bean accessions or cultivars.
ACCESSION #
91965194

 

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