Mapping and validation of QTLs for rice sheath blight resistance

Fumio Taguchi-Shiobara; Hidenobu Ozaki; Hiroyuki Sato; Hiroaki Maeda; Yoichiro Kojima; Takeshi Ebitani; Masahiro Yano
September 2013
Breeding Science;Sep2013, Vol. 63 Issue 3, p301
Academic Journal
Sheath blight, caused by Rhizoctonia solani, is one of the most serious diseases of rice. Among 33 rice ac-cessions, mainly from National Institute of Agrobiological Sciences (NIAS) Core Collection, we found three landraces from the Himalayas--Jarjan, Nepal 555 and Nepal 8--with resistance to sheath blight in 3 years' field testing. Backcrossed inbred lines (BILs) derived from a cross between Jarjan and the leading Japanese cultivar Koshihikari were used in QTL analyses. Since later-heading lines show fewer lesions, we used only earlier-heading BILs to avoid association with heading date. We detected eight QTLs; the Jarjan allele of three of these increased resistance. Only one QTL, on chromosome 9 (between markers Nag08KK18184 and Nag08KK 18871), was detected in all 3 years. Chromosome segment substitution lines (CSSLs) carrying it showed resistance in field tests. Thirty F2 lines derived from a cross between Koshihikari and one CSSL sup-ported the QTL.


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