Construction of integrated linkage map of a recombinant inbred line population of white lupin (Lupinus albus L.)

Vipin, Cina Ann; Luckett, David J.; Harper, John D. I.; Ash, Gavin J.; Kilian, Andrzej; Ellwood, Simon R.; Phan, Huyen T. T.; Raman, Harsh
September 2013
Breeding Science;Sep2013, Vol. 63 Issue 3, p292
Academic Journal
We report the development of a Diversity Arrays Technology (DArT) marker panel and its utilisation in the development of an integrated genetic linkage map of white lupin (Lupinus albus L.) using an Fg recombinant inbred line population derived from Kiev Mutant/P27174. One hundred and thirty-six DArT markers were merged into the first genetic linkage map composed of 220 amplified fragment length polymorphisms (AFLPs) and 105 genie markers. The integrated map consists of 38 linkage groups of 441 markers and spans a total length of 2,169 cM, with an average interval size of 4.6 cM. The DArT markers exhibited good ge-nome coverage and were associated with previously identified genie and AFLP markers linked with quanti-tative trait loci for anthracnose resistance, flowering time and alkaloid content. The improved genetic linkage map of white lupin will aid in the identification of markers for traits of interest and future syntenic studies.


Related Articles

  • Molecular mapping of the Rf fertility restoration gene to facilitate its utilization in breeding confection sunflower. Liu, Zhao; Mulpuri, Sujatha; Feng, Jiuhuan; Vick, Brady; Jan, Chao-Chien // Molecular Breeding;Feb2012, Vol. 29 Issue 2, p275 

    The inheritance of a previously identified dominant Rf gene in the confection sunflower line RHA 280 has been determined and designated as Rf. This study reports the mapping of the Rf locus using an F2 population of 227 individuals derived from CMS HA 89-3149 × RHA 280. Bulked segregant...

  • Population structure and linkage disequilibrium in Lupinus albus L. germplasm and its implication for association mapping. Iqbal, Muhammad; Mamidi, Sujan; Ahsan, Rubina; Kianian, Shahryar; Coyne, Clarice; Hamama, Anwar; Narina, Satya; Bhardwaj, Harbans // Theoretical & Applied Genetics;Aug2012, Vol. 125 Issue 3, p517 

    White lupin ( Lupinus albus L.) has been around since 300 B.C. and is recognized for its ability to grow on poor soils and application as green manure in addition to seed harvest. The seed has very high levels of protein (33-47 %) and oil (6-13 %). It also has many secondary metabolites that are...

  • Genetic mapping and QTL analysis for sugar yield-related traits in sugarcane. Singh, Ram; Singh, Sujeet; Tiwari, Dinesh; Srivastava, Shraddha; Singh, Surendra; Sharma, Mukut; Singh, Rakesh; Mohapatra, Trilochan; Singh, Nagendra // Euphytica;Jun2013, Vol. 191 Issue 3, p333 

    Genetic improvement of sugar content in sugarcane would benefit from the availability of sufficient DNA markers and a genetic map. Genetic linkage maps were constructed to identify quantitative trait loci (QTLs) for seedling brix (SB), brix (B), sucrose percent in juice (SUC), stalk number (SN),...

  • Fine mapping of the Rrs1 resistance locus against scald in two large populations derived from Spanish barley landraces. Hofmann, Kerstin; Silvar, Cristina; Casas, Ana M.; Herz, Markus; Büttner, Bianca; Gracia, M. Pilar; Contreras-Moreira, Bruno; Wallwork, Hugh; Igartua, Ernesto; Schweizer, Günther // Theoretical & Applied Genetics;Dec2013, Vol. 126 Issue 12, p3091 

    Key message: In two Spanish barley landraces with outstanding resistance to scald, theRrs1 Rh4locus was fine mapped including all known markers used in previous studies and closely linked markers were developed.Scald, caused by Rhynchosporium commune, is one of the most prevalent barley diseases...

  • Molecular mapping of QTLs for resistance to early and late Fusarium wilt in chickpea. PATIL, BASAVANAGOUDA SIDDANAGOUDA; RAVIKUMAR, RAMAPUR LAXMIPATHI; BHAT, JAYANT SUBRAY; SOREGAON, CHANDRAKANT DHARMARAYA // Czech Journal of Genetics & Plant Breeding;2014, Vol. 50 Issue 2, p171 

    A molecular map of chickpea was constructed using F9:F10 recombinant inbred lines from an intraspecific cross between Fusarium wilt susceptible (JG 62) and resistant (WR 315) genotypes. A total of 23 markers with LOD scores of ï¹¥ 3.0 were mapped on the recombinant inbred lines (RILs). Twenty...

  • An enhanced microsatellite map of diploid Fragaria. Sargent, D. J.; Clarke, J.; Simpson, D. W.; Tobutt, K. R.; Arús, P.; Monfort, A.; Vilanova, S.; Denoyes-Rothan, B.; Rousseau, M.; Folta, K. M.; Bassil, N. V.; Battey, N. H. // Theoretical & Applied Genetics;May2006, Vol. 112 Issue 7, p1349 

    A total of 45 microsatellites (SSRs) were developed for mapping in Fragaria. They included 31 newly isolated codominant genomic SSRs from F. nubicola and a further 14 SSRs, derived from an expressed sequence tagged library (EST-SSRs) of the cultivated strawberry, F. × ananassa. These, and an...

  • Genetic diversity of wild Thymus capitatus (Lamiaceae) in Tunisia using molecular markers. Hadj Ali, Imen Ben El; Guetat, Arbi; Boussaid, Mohamed // Dendrobiology;Nov2012, Vol. 68, p89 

    In Tunisia, Thymus capitatus L. populations are severely destroyed due to deforestation and over-collecting. The species occurs in small scatteredpopulations decreasing progressively in size. Yet, no conservation or improvement programs are attemptedto preserve andpromote the potential value of...

  • A framework genetic map of Muscadinia rotundifolia. Riaz, S.; Hu, R.; Walker, M. // Theoretical & Applied Genetics;Oct2012, Vol. 125 Issue 6, p1195 

    This study presents a framework linkage map based on microsatellite markers for Muscadinia rotundifolia (1 n = 20). The mapping population consisted of 206 progeny generated from a cross of two M. rotundifolia varieties, 'Fry' and 'Trayshed'. A total of 884 primers were tested for their ability...

  • Tagging and mapping candidate loci for vernalization and flower initiation in hexaploid oat. Nava, Itamar; Wight, Charlene; Pacheco, Marcelo; Federizzi, Luiz; Tinker, Nicholas // Molecular Breeding;Oct2012, Vol. 30 Issue 3, p1295 

    Flowering time is a decisive factor in the adaptation of oat. Some oat varieties require low temperatures for floral initiation, a process called vernalization. The objectives of this study were to clone, characterize, and map genes associated with vernalization in oat, and to identify markers...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics