Single-laboratory validation of a multiplex flow cytometric immunoassay for the simultaneous detection of coccidiostats in eggs and feed

Bienenmann-Ploum, Monique E.; Vincent, Ursula; Campbell, Katrina; Huet, Anne-Catherine; Haasnoot, Willem; Delahaut, Philippe; Stolker, Linda (A.)A.M.; Elliott, Christopher T.; Nielen, Michel W. F.
November 2013
Analytical & Bioanalytical Chemistry;Nov2013, Vol. 405 Issue 27, p9571
Academic Journal
Coccidiostats are authorized in the European Union (EU) to be used as poultry feed additives. Maximum (residue) levels (M(R)Ls) have been set within the EU for consumer and animal protection against unintended carry-over, and monitoring is compulsory. This paper describes the single-laboratory validation of a previously developed multiplex flow cytometric immunoassay (FCIA) as screening method for coccidiostats in eggs and feed and provides and compares different approaches for the calculation of the cut-off levels which are not described in detail within Commission Decision 2002/657/EC. Comparable results were obtained between the statistical (reference) approach and the rapid approaches. With the most rapid approach, the cut-off levels for narasin/salinomycin, lasalocid, diclazuril, nicarbazin (DNC) and monensin in egg, calculated as percentages of inhibition (%B/B0), were 60, 32, 76, 80 and 84, respectively. In feed, the cut-off levels for narasin/salinomycin, lasalocid, nicarbazin (DNC) and monensin were 70, 64, 72 and 78, respectively, and could not be determined for diclazuril. For all analytes, except for diclazuril in feed, the rate of false positives (false non-compliant) in blank samples was lower than 1 %, and the rate of false negatives (false compliant) at the M(R)Ls was below 5 %. Additionally, very good correlations ( r ranging from 0.994 to 0.9994) were observed between two different analysers, a sophisticated flow cytometer (FlexMAP 3D ®) and a more cost-efficient and transportable planar imaging detector (MAGPIX ®), hence demonstrating adequate transferability.


Related Articles

  • Immunoassays, drugs-of-abuse screening, and quality assurance. Ruzich, Kathy // MLO: Medical Laboratory Observer;Dec2011, Vol. 43 Issue 12, p40 

    The article presents information on the use of immunoassay technologies in drugs-of-abuse screening and therapeutic drug monitoring (TDM). It informs that the U.S. Food and Drug Administration has recently approved the Cloned Enzyme Donor Immunoassay (CEDIA) Oral Fluid immunoassays calibrators...

  • Oxycodone: actions, metabolism, elimination, and detection. Rodríguez, María Luz // MLO: Medical Laboratory Observer;May2013, Vol. 45 Issue 5, p24 

    The article discusses oxycodone, an analgesic medication which was developed as one of several new semi-synthetic opioids in an attempt to improve on the existing opioids: morphine, diacetylmorphine, and codeine. It mentions that oxycodone is effective for managing moderate to moderately severe...

  • Catastrophic Complications of Intravenous Line Flushing with Unfractionated Heparin. Colucci, Giuseppe; Jahns, Maximilian; Silzle, Tobias; Alberio, Lorenzo // International Journal of Clinical Medicine;Jan2012, Vol. 3 Issue 1, p36 

    Heparin-induced thrombocytopenia (HIT) is a clinicopathologic syndrome because diagnosis is based on both clinical symptoms and laboratory data. We report a patient with multiple thromboembolic complications after daily flushing of intravenous line with small amounts of unfractionated heparin...

  • Belief is only half the truth - or why screening for heterophilic antibody interference in certain assays makes double sense. Bjerner, Johan; Bolstad, Nils; Piehler, Armin // Annals of Clinical Biochemistry;Jul2012, Vol. 49 Issue 4, p381 

    Background: Interference in immunoassays may cause both false-negative and false-positive results. It may be detected using a number of affirmative tests such as reanalysis of certain samples using different assay platforms with known bias, after the addition of blocker antibodies, or assessment...

  • Systemic Inflammation or Monoclonal Gammopathy? Yu Chen; Watts, Gail // Archives of Pathology & Laboratory Medicine;Dec2011, Vol. 135 Issue 12, p1527 

    A letter to the editor is presented in which the authors offer information on systemic inflammation or monoclonal gammopathy and how the mirror cerebrospinal fluid (CSF)/serum pattern is indicative of serum proteins entering the CSF by passive diffusion or disruption of the blood-brain barrier.

  • Advancing the field of cancer immunotherapy MIATA consensus guidelines become available to improve data reporting and interpretation for T-cell immune monitoring. Hoos, Axel; Janetzki, Sylvia; Britten, Cedrik M. // OncoImmunology;Dec2012, Vol. 1 Issue 9, p1 

    The authors reflect on the Minimal Information about T cell assays (MIATA) guidelines for the improvement of interpretation and reporting of data on t-cell immune monitoring. They state that MIATA guidelines in clinical trials require information on sample, assay, data acquisition, results, and...

  • Evaluation of acute immunotoxicity of alachlor in male F344/N Rats. Biagini, Raymond; Henningsen, Gerry; MacKenzie, Barbara; Sanderson, Wayne; Robertson, Shirley; Baumgardner, Eric // Bulletin of Environmental Contamination & Toxicology;Feb1993, Vol. 50 Issue 2, p266 

    The article presents a study which aims to investigate the acute immunotoxicity of alachlor, a type of herbicides in the U.S., in male rats. The study analyzes a multiple immunoassay model and other chemicals such as reagent grade in two groups of laboratory animals. Results show that the...

  • Development of a colloidal gold-based lateral-flow immunoassay for the rapid simultaneous detection of clenbuterol and ractopamine in swine urine. Ming-Zhou Zhang; Min-Zi Wang; Zong-Lun Chen; Jie-Hong Fang; Mei-Ming Fang; Jun Liu; Xiao-Ping Yu // Analytical & Bioanalytical Chemistry;Dec2009, Vol. 395 Issue 8, p2591 

    A multianalyte lateral-flow immunochromatographic technique using colloidal gold-labeled polyclonal antibodies was developed for the rapid simultaneous detection of clenbuterol and ractopamine. The assay procedure could be accomplished within 5 min, and the results of this qualitative one-step...

  • Development of Taste Masked Oral Formulation of Ornidazole. SHISHU; KAMALPREET; KAPOOR, V. R. // Indian Journal of Pharmaceutical Sciences;Mar/Apr2010, Vol. 72 Issue 2, p211 

    Taste masked microspheres of ornidazole were prepared using amino alkyl methacrylate copolymers (Eudragit E-100) by solvent evaporation technique. Taste assessment of these microspheres was done by both spectrophotometric taste evaluation technique and panel testing. Compressed tablets of taste...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics