TITLE

Comparison of Enrichment Broths for the Recovery of Healthy and Heat-Injured Salmonella Typhimurium on Raw Duck Wings

AUTHOR(S)
QIANWANG ZHENG; BUSTANDI, CAROLINE; YISHAN YANG; SCHNEIDER, KEITH R.; HYUN-GYUN YUK
PUB. DATE
November 2013
SOURCE
Journal of Food Protection;Nov2013, Vol. 76 Issue 11, p1963
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
This study was performed to optimize Salmonella Typhimurium recovery from raw duck wings with five nonselective broths (buffered peptone water, tryptic soy broth, lactose broth, universal preenrichment broth, nutrient broth) and four selective broths (selenite broth, BAX System MP media [MP], Salmonella AD media [AD], ONE broth-Salmonella [OB]). Healthy or heat-injured (50 and 85% injury) cells were inoculated at a level of 10², 10¹, or 100 CFU/25 g on raw duck wings. Growth was modeled using DMfit with four growth parameters: lag-phase duration, maximum growth rate, doubling time, and maximum population density. Most enrichments were able to recover Salmonella Typhimurium to greater than 6 log CFU/ml. AD, MP, and OB had significantly (P < 0.05) higher maximum growth rate (0.9 to 1.0/h) and lower doubling time (0.7 to 0.8 h). Buffered peptone water, AD, MP, and OB recovered healthy and 50%-injured cells at low inoculum levels to more than 6.0 log CFU/ml; OB achieved the greatest recovery (7.6 and 7.9 log CFU/ml), following 24 h of incubation. The 85%-injured cells at 100 and 10¹ CFU/25 g, however, were only recovered in OB, reaching 7.3 and 7.5 log CFU/ml, respectively. These results suggest that OB may be an appropriate enrichment broth for the recovery of Salmonella Typhimurium from raw duck wings in standard diagnostic tests or other rapid detection methods, to avoid false-negative results.
ACCESSION #
91926389

 

Related Articles

  • Prevalence of Salmonella Isolates and Antimicrobial Resistance in Poultry Meat from South Korea. RAN-HEE YOON; SE-YEOUN CHA; BAI WEI; JAE-HEE ROH; HYE-SUK SEO; JAE-YOUNG OH; HYUNG-KWAN JANG // Journal of Food Protection;Sep2014, Vol. 77 Issue 9, p1579 

    Contamination of Salmonella was assessed in duck and chicken meat collected from supermarkets, traditional markets, internet shopping malls, and wholesale markets in Jeonlado, South Korea, in 2013. Salmonella contamination was found in 51.3% of duck meat samples and 3.7% of chicken meat samples....

  • Clinicians urged to warn patients about pet frogs, salmonella risk. Shimshony, Arnon // Infectious Diseases in Children;Jun2013, Vol. 26 Issue 6, p6 

    The article reports on the study by researchers, which showed that the outbreak of Salmonella typhimurium in the U.S. in 2008-2011 was linked to African dwarf frogs, thus, pediatricians are advised to ask their patients and parents about possible exposure to the frogs and other amphibians.

  • Humans and animals share their diseases. NEWPORT, ALAN // Carolina Virginia Farmer;Apr2013, Vol. 31 Issue 4, Special section p7 

    The article discusses the results of a study of the Salmonella typhimurium DT104 strain which showed animals and humans pass the salmonella disease between species, performed by veterinarian Stuart Reid who claimed that the research proved the interrelation between the ecosystem and all its parts.

  • CONTROL OF SALMONELLA ACTIVITY IN RATS BY PULSED ELF MAGNETIC FIELD (IN VIVO STUDY). Ali, Fadel Mohamed; Gawish, Azza Mahmoud; Saddek Osman, Massarat Bakr; Abdelbacki, Ashraf Mahmoud; El- Sharkawy, Amira Hamdi // Journal of International Dental & Medical Research;2012, Vol. 5 Issue 2, p129 

    In this work, the frequency of the magnetic impulses that interfere with the bioelectric signals generated during salmonella typhimurium (STm) cellular division is investigated. The experiment was expanded to in vivo study for the obtained data in which rats were infected with STm and then whole...

  • TTSS2-deficient hha mutant of Salmonella Typhimurium exhibits significant systemic attenuation in immunocompromised hosts. Vishwakarma, Vikalp; Niladri Bhusan Pati; Ray, Shilpa; Das, Susmita; Suar, Mrutyunjay // Virulence;2/15/2014, Vol. 5 Issue 2, p311 

    Non-typhoidal Salmonella (NTS) infections are emerging as leading problem worldwide and the variations in host immune status append to the concern of NTS. Salmonella enterica serovar Typhimurium is one of the causative agents of NTS infections and has been extensively studied. The inactivation...

  • Mutagenic activity of incense smoke in Salmonella typhimurium. Rasmussen, Ronald // Bulletin of Environmental Contamination & Toxicology;May1987, Vol. 38 Issue 5, p827 

    The article discusses the study on mutagenic action of nitrosamines and other cigarette smoke elements in Salmonella typhimurium. It states that researchers had conducted mutagenesis assessments in order to determine the effect of incense smoke to the said bacteria. It says that the study shows...

  • Humans and animals share their diseases. NEWPORT, ALAN // American Agriculturist (0161-8237);Apr2013, Vol. 210 Issue 4, pBP7 

    The article discusses findings of an epidemiological study indicating that animals and humans are passing between species the disease of one strain of salmonella called Salmonella typhimurium DT104 as a drug-resistant and virulent pathogen.

  • Possible Mechanisms of Antimutagenicity in Fermented Soymilk Prepared with a Coculture of Streptococcus infantis and Bifidobacteriurn infantis. Meng-Li Hsieh; Fang, Shao W.; Roch-Chui Yu; Cheng-Chun Chou // Journal of Food Protection;Apr2007, Vol. 70 Issue 4, p1025 

    The possible mechanisms of antimutagenicity against 4-nitroquinoline-N-oxide (4-NQO; a direct mutagen) and 3,2′-dimethyl-4-amino-biphenyl (DMAB; an indirect mutagen) were examined in fermented soymilk prepared with a coculture of Streptococcus thermophilus and Bifidobacterium infantis. The...

  • The proline-rich peptide Bac7(1-35) reduces mortality from Salmonella typhimurium in a mouse model of infection. Benincasa, Monica; Pelillo, Chiara; Zorzet, Sonia; Garrovo, Chiara; Biffi, Stefania; Gennaro, Renato; Scocchi, Marco // BMC Microbiology;2010, Vol. 10, p178 

    Background: Bac7 is a proline-rich peptide with a potent in vitro antimicrobial activity against Gram-negative bacteria. Here we investigated its activity in biological fluids and in vivo using a mouse model of S. typhimurium infection. Results: The efficacy of the active 1-35 fragment of Bac7...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics