TITLE

Development of Microsatellite Markers in Pungtungia herzi Using Next-Generation Sequencing and Cross-Species Amplification in the Genus Pseudopungtungia

AUTHOR(S)
Young-Eun Yun; Jeong-Nam Yu; Sang Ki Kim; Ui Wook Hwang; Kwak, Myounghai
PUB. DATE
October 2013
SOURCE
International Journal of Molecular Sciences;Oct2013, Vol. 14 Issue 10, p19923
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Nuclear microsatellite markers for Pungtungia herzi were developed using a combination of next-generation sequencing and Sanger sequencing. One hundred primer sets in the flanking region of dinucleotide and trinucleotide repeat motifs were designed and tested for efficiency in polymerase chain reaction amplification. Of these primer sets, 16 new markers (16%) were successfully amplified with unambiguous polymorphic alleles in 16 individuals of Pungtungia herzi. Cross-species amplification with these markers was then examined in two related species, Pseudopungtungia nigra and Pseudopungtungia tenuicorpa. Fifteen and 11 primer pairs resulted in successful amplification in Pseudopungtungia nigra and Pseudopungtungia tenuicorpa, respectively, with various polymorphisms, ranging from one allele (monomorphic) to 11 alleles per marker. These results indicated that developing microsatellite markers for cross-amplification from a species that is abundant and phylogenetically close to the species of interest is a good alternative when tissue samples of an endangered species are insufficient to develop microsatellites.
ACCESSION #
91701748

 

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