TITLE

Cell-to-Cell Contact of Human Monocytes with Infected Arterial Smooth-Muscle Cells Enhances Growth of Chlamydia pneumoniae

AUTHOR(S)
Puolakkainen, Mirja; Ann Campbell, Lee; Tsun-Mei Lin; Richards, Theresa; Patton, Dorothy L.; Cho-Chou Kuo
PUB. DATE
February 2003
SOURCE
Journal of Infectious Diseases;2/1/2003, Vol. 187 Issue 3, p435
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Chlamydia pneumoniae can infect arterial cells. It has been shown that coculture of human monocytes (U937) and endothelial cells promotes infection of C. pneumoniae in endothelial cells and that the enhancement was mediated by a soluble factor (insulin-like growth factor 2) secreted by monocytes. In this study, it is shown that coculture of monocytes with C. pneumoniae enhances infection of C. pneumoniae in arterial smooth-muscle cells 5.3-fold at a monocyte-to-smooth-muscle cell ratio of 5. However, unlike endothelial cells, no enhancement was observed if monocytes were placed in cell culture inserts or if conditioned medium from monocyte cultures was used, which suggests that cell-to-cell contact is critical. The addition of mannose 6-phosphate or octyl glucoside, a nonionic detergent containing a sugar group, to cocultures inhibited the enhancement. These findings suggest that the monocyte-smooth-muscle cell interaction may be mediated by mannose 6-phosphate receptors present on monocytes.
ACCESSION #
9152552

 

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics