TITLE

Cloning and characterization of human complement component C7 promoter

AUTHOR(S)
González, S; Martínez-Borra, J; López-Larrea, C
PUB. DATE
January 2003
SOURCE
Genes & Immunity;Jan2003, Vol. 4 Issue 1, p54
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
To study the transcriptional regulation of the human complement component C7, a 1 kb promoter fragment was cloned and the transcription start site was determined. C7 is expressed by the hepatoma-derived cell line Hep-3B, but not by Hep-G2. Transfection of these cell lines with different C7 promoter -- luciferase constructs demonstrated that 1 kb of the 5'-flanking region contains the necessary elements for driving C7 transcription in a tissue-specific manner and showed that the sequence between -29/+102 retained the majority of C7 promoter activity in Hep-3B. Electrophoretic mobility shift assays suggested that the binding of the C/EBPα transcription factor to a C/EBP sequence located at +42 is essential for C7 expression. To investigate whether the absence of C/EBPα expression in Hep-G2 cells is responsible for the lack of C7 transcription, Hep-G2 cells were transfected with a C/EBPα expression vector. C/EBPα transactivated the C7 luciferase reported gene and restored the C7 expression in Hep-G2 cells.
ACCESSION #
9137695

 

Related Articles

  • Hypertension.  // Clinical & Investigative Medicine;Aug97 Supplement, Vol. 20, pS39 

    Presents an abstract of the research manuscript `Molecular cloning of a novel protein that inhibits the stimulatory effect of isoproterenol on the expression of the angiotensinogen gene in mouse hepatoma cells in vitro,' by X. Chen, J. Wu et al.

  • Differentially profiling the low-expression transcriptomes of human hepatoma using a novel SSH/microarray approach. Pan, Yi-Shin; Lee, Yun-Shien; Lee, Yung-Lin; Lee, Wei-Chen; Hsieh, Sen-Yung // BMC Genomics;2006, Vol. 7, p131 

    Background: The main limitation in performing genome-wide gene-expression profiling is the assay of low-expression genes. Approaches with high throughput and high sensitivity for assaying low-expression transcripts are urgently needed for functional genomic studies. Combination of the...

  • Cloning and characterization of a maltotriose-producing a-amylase gene from Thermobifida fusca. Chao-Hsun Yang; Wen-Hsiung Liu // Journal of Industrial Microbiology & Biotechnology;Apr2007, Vol. 34 Issue 4, p325 

    The gene ( tfa), encoding a maltotriose-producing a-amylase from Thermobifida fusca NTU22, was cloned, sequenced and expressed in Escherichia coli. The gene consists of 1,815 base pairs and encodes a protein of 605 amino acids. The base composition of the tfa coding sequence is 69% G+C and the...

  • Designing and constructing an 100 bp DNA Ladder by combining PCR and enzyme digestion methods. Saidijam, Massoud; Shahreza, Hossein Khanahmad; Tehrani, Zahra Rikhtegaran; Karimizare, Sakineh; Shabab, Nooshin; Behdani, Mehdi // Tehran University Medical Journal;May2011, Vol. 69 Issue 2, p75 

    Background: Molecular DNA markers are one of the most important tools in molecular biology labs. The size of DNA molecules is determined by comparing them with known bands of markers during gel electrophoresis. There are many different protocols to produce these kinds of molecular markers. In...

  • An approach for isolating high-molecular-weight glutenin subunit genes using monoclonal antibodies. Wang, H. Q.; Zhang, Z. Y. // Genome;Feb2006, Vol. 49 Issue 2, p181 

    High-molecular-weight glutenin subunits (HMW-GSs) play an important role in the breadmaking quality of wheat flour. In China, cultivars such as Triticum aestivum 'Xiaoyan No. 6' carrying the 1Bx14 and 1By15 glutenin subunits usually have attributes that result in high-quality bread and noodles....

  • MOLECULAR GENE CLONING OF NICOTINE-DEHYDROGENASE FROM THE pAO1 MEGAPLASMID OF ARTHROBACTER NICOTINOVORANS. ANDREI, ANDREEA; MIHĂŞAN, MARIUS // Analele Stiintifice ale Universitatii Alexandru Ioan Cuza din Ia;Sep2013, Vol. 14 Issue 3, p15 

    6-hydroxi-L-nicotine (6HNic) has an important potential as a drug for neuro-degenerative disorders and a suitable simple and reliable method for obtaining contaminant-free 6HNic preparations is required. Here, we envision the in-vitro production of 6HNic by using purified nicotine-dehydrogenase...

  • Monoclonal antibodies to secreted antigens of Brugia malayi define a cross-reactive non-phosphocholine determinant on helminth parasites. Lala, Renu B. // Immunology & Cell Biology;Apr1991, Vol. 69 Issue 2, p127 

    The excretory-secretory (ES) antigens of the filarial parasite Brugia malayi adult (BmA) and microfilariae (MF) were analysed for differences in their protein composition by two-dimensional gel electrophoresis. Both BmA and MF biosynthetically labelled with [³H]-leucine released a 200 kD...

  • Cloning DNA through the use of Recombinant DNA Technology. Rotenhoffen, Erik // School of Doctoral Studies European Union Journal;2010, Issue 2, p128 

    Recombinant DNA technology was used to clone specific DNA fragments. Before the technology was used, a basic understanding of the parts and details of the procedure were investigated. It was found that the larger the genome of an organism, the greater its overall complexity relative to other...

  • Substrate Promiscuity of N-Acetylhexosamine 1-Kinases. Yanhong Li; Hai Yu; Yi Chen; Kam Lau; Li Cai; Hongzhi Cao; Tiwari, Vinod Kumar; Jingyao Qu; Thon, Vireak; Wang, Peng George; Xi Chen // Molecules;Aug2011, Vol. 16 Issue 8, p6396 

    N-Acetylhexosamine 1-kinase (NahK) catalyzes the direct addition of a phosphate from adenosine 5'-triphosphate (ATP) to the anomeric position of N-acetylhexosamine and shows similar activity towards N-acetylglucosamine (GlcNAc) and N-acetylgalactosamine (GalNAc). Herein we report the cloning,...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics