TITLE

Use of Fluorescent Dyes for Readily Recognizing Sperm Damage

AUTHOR(S)
Ibrahim Farah, Omar; Li Cuiling; Wang Jiaojiao; Zhang Huiping
PUB. DATE
July 2013
SOURCE
Journal of Reproduction & Infertility;Jul-Sep2013, Vol. 14 Issue 3, p120
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Sperm is produced by the testis and mature in the epididymis. For having a successful conception, the fertilizing sperm should have functional competent membranes, intact acrosome, functional mitochondria and an intact haploid genome. The effects of genetic and environmental factors result in sperm vulnerability to damage in the process of spermatogenesis and maturation. In recent years, the feasibility of detecting sperm damage is enhanced through the advances in technologies like fluoscerent staining techniques assisted with fluorescence microscope, flow cytometry and computer analysis systems. Fluoscerent staining techniques involve the use of fluorescent dyes, either directly or indirectly for binding them with some ingredients of sperm and evaluating the damage of the structure or function of the sperm, i.e. membrane, acrosome, mitochondria, chromosome or DNA.
ACCESSION #
90545244

 

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