TITLE

A reversible gene trap collection empowers haploid genetics in human cells

AUTHOR(S)
Bürckstümmer, Tilmann; Banning, Carina; Hainzl, Philipp; Schobesberger, Richard; Kerzendorfer, Claudia; Pauler, Florian M; Chen, Doris; Them, Nicole; Schischlik, Fiorella; Rebsamen, Manuele; Smida, Michal; de la Cruz, Ferran Fece; Lapao, Ana; Liszt, Melissa; Eizinger, Benjamin; Guenzl, Philipp M; Blomen, Vincent A; Konopka, Tomasz; Gapp, Bianca; Parapatics, Katja
PUB. DATE
October 2013
SOURCE
Nature Methods;Oct2013, Vol. 10 Issue 10, p965
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Knockout collections are invaluable tools for studying model organisms such as yeast. However, there are no large-scale knockout collections of human cells. Using gene-trap mutagenesis in near-haploid human cells, we established a platform to generate and isolate individual 'gene-trapped cells' and used it to prepare a collection of human cell lines carrying single gene-trap insertions. In most cases, the insertion can be reversed. This growing library covers 3,396 genes, one-third of the expressed genome, is DNA-barcoded and allows systematic screens for a wide variety of cellular phenotypes. We examined cellular responses to TNF-α, TGF-β, IFN-γ and TNF-related apoptosis-inducing ligand (TRAIL), to illustrate the value of this unique collection of isogenic human cell lines.
ACCESSION #
90479577

 

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