Overexpression of mtlD gene in potato (solanum tuberosum L.), cv. Arinda improves salt tolerance

Askari, Aliakbar; Pepoyan, Astghik
September 2012
Advances in Environmental Biology;Sep2012, Vol. 6 Issue 10, p2646
Academic Journal
Potato as one of the world's main tuber crops of the solanaceae family plays an important role in the economics scene. Since one of the major reasons for yield decreasing in the world are abiotic stresses. The aim of this research was producing transgenic potatoes with more tolerance to salinity stress and studying GMO potato features. To produce GMO plants, new gene (mtlD, mannitol -1- phosphate dehydrogenase, E.C. was transferred to a plant by using agrobacterium plasmid. For the production of transformed potato plant resistant to salinity stress, mannitol, 1-phosphate dehydrogenase gene (mtlD, E.C. was used. At first mtld gene was isolated from pCabmtld plasmid of E. Coli and then was cloned in PBI121 plasmid. The promoters that have been most commonly used in the production of abiotic stress tolerant plants so far, include the CaMV 35S (Bhatnagar-mathur et al., 2007). This vector was inserted into Agrobacterium tumefaciens which was used for producing transformed potato plant and was stored in 28°c temperature. The freeze-thaw method (Holsters et al., 1978) was used for replacement of recombinant binary vector PBI-VHH into Agrobacterium tumefaciens strains LBA4404.. Existence of recombinant gene in transgenic plants was confirmed by PCR technique. For final confirmation, the features of transgenic potato were studied. Briefly, the transgenic potatoes and non- transgenic potatoes lines showed the different amounts of tolerance to salinity stress because the transgenic lines produced mannitol which increased osmotic pressure in salinity stress. The salt tolerance of transgenic potato (+mtlD) was recorded higher than that of non- transgenic potato (-mtlD). Growth analyses include CGR (Crop Growth Rate) and RGR (Relative Growth Rate) in transgenic and non- transgenic potatoes lines were showed that +mtlD potatoes lines grew better than --mtlD potatoes lines in salinity stress. This approved that +mtlD had more tolerance to salinity stress due to production of mannitol. The comparison of linear regression equations of transgenic (+mtlD) and non- transgenic potatoes (-mtlD) showed the more tolerance of +mtlD to salinity stress than --mtlD potatoes. Osmotic pressure in this GMO plant was increased by producing of mannitol and consequently producing mannitol certify that the mtlD gene was successfully transferred to potato.


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