TITLE

Ca-dependent suicidal erythrocyte death following zearalenone exposure

AUTHOR(S)
Jilani, Kashif; Lang, Florian
PUB. DATE
October 2013
SOURCE
Archives of Toxicology;Oct2013, Vol. 87 Issue 10, p1821
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Zearalenone, a cereal mycotoxin with mycoestrogen activity and effect on fertility, is known to trigger apoptosis of a variety of nucleated cell types including hematopoietic progenitor cells. In analogy to apoptosis of nucleated cells, eryptosis, the suicidal death of erythrocytes, leads to cell shrinkage and cell membrane scrambling with phosphatidylserine exposure at the erythrocyte surface. The most important stimulator of eryptosis is an increase in cytosolic Ca activity ([Ca]). The present study explored whether zearalenone triggers eryptosis. Erythrocyte volume was estimated from forward scatter, phosphatidylserine exposure at the erythrocyte surface from annexin-V binding, hemolysis from hemoglobin release, and [Ca] from Fluo3 fluorescence. A 48-h exposure to zearalenone (≥25 μM) was followed by a significant increase in [Ca] and annexin-V binding, and a significant decrease in forward scatter. The effect on annexin-V binding was significantly blunted in the nominal absence of extracellular Ca. Zearalenone stimulates the suicidal erythrocyte death, an effect at least partially due to stimulation of Ca entry.
ACCESSION #
90375067

 

Related Articles

  • Suicidal Erythrocyte Death Following Cellular K Loss. Schneider, Juliane; Nicolay, Jan P.; Föller, Michael; Wieder, Thomas; Lang, Florian // Cellular Physiology & Biochemistry (Karger AG);2007, Vol. 20 Issue 1-4, p035 

    Hallmarks of apoptosis include cell shrinkage, which is at least partially due to cellular K+ loss. The decline of cellular K+ concentration has been suggested to participate in the triggering of apoptosis. Suicidal erythrocyte death or eryptosis is triggered by increased cytosolic Ca2+ activity...

  • In Vitro Sensitization of Erythrocytes to Programmed Cell Death Following Baicalein Treatment. Bissinger, Rosi; Malik, Abaid; Honisch, Sabina; Warsi, Jamshed; Jilani, Kashif; Lang, Florian // Toxins;Sep2014, Vol. 6 Issue 9, p2771 

    The polyphenolic flavonoid Baicalein has been shown to trigger suicidal death or apoptosis of tumor cells and is thus considered for the prevention and treatment of malignancy. Similar to apoptosis of nucleated cells, erythrocytes may enter eryptosis, the suicidal erythrocyte death characterized...

  • Stimulating Effect of Manumycin A on Suicidal Erythrocyte Death. Egler, Jasmin; Zierle, Jens; Lang, Florian // Cellular Physiology & Biochemistry (Karger AG);Mar2016, Vol. 38 Issue 3, p1147 

    Background/Aims: The streptomycete derived farnesyltransferase inhibitor Manumycin A triggers apoptosis of tumor cells and is thus considered for the treatment of malignancy. The present study explored whether Manumycin A could similarly stimulate eryptosis, the suicidal death of erythrocytes...

  • Janus Kinase 3 is Expressed in Erythrocytes, Phosphorylated Upon Energy Depletion and Involved in the Regulation of Suicidal Erythrocyte Death. Bhavsar, Shefalee K.; Gu, Shuchen; Bobbala, Diwakar; Lang, Florian // Cellular Physiology & Biochemistry (Karger AG);2011, Vol. 27 Issue 5, p547 

    Janus kinase 3, a tyrosine kinase expressed in haematopoetic tissues, plays a decisive role in T-lymphocyte survival. JAK3 deficiency leads to (Severe) Combined Immunodeficiency (SCID) resulting from enhanced lymphocyte apoptosis. JAK3 is activated by phosphorylation. Nothing is known about...

  • Triggering of Suicidal Erythrocyte Death Following Boswellic Acid Exposure. Calabrò, Salvatrice; alzoubi, Kousi; Faggio, Caterina; Laufer, Stefan; Lang, Florian // Cellular Physiology & Biochemistry (Karger AG);Aug2015, Vol. 37 Issue 1, p131 

    Background/Aims: The antinflammatory natural product boswellic acid is effective against cancer at least in part by inducing tumor cell apoptosis. Similar to apoptosis of nucleated cells erythrocytes may enter eryptosis, a suicidal death characterized by cell shrinkage and cell membrane...

  • Phosphatidylserine exposure during apoptosis reflects bidirectional trafficking between plasma membrane and cytoplasm. Lee, S-H; Meng, X W; Flatten, K S; Loegering, D A; Kaufmann, S H // Cell Death & Differentiation;Jan2013, Vol. 20 Issue 1, p64 

    Phosphatidylserine (PS) exposure on the external leaflet of the plasma membrane is widely observed during apoptosis and forms the basis for the annexin V binding assay to detect apoptotic cell death. Current efforts to explain PS exposure focus on two potential mechanisms, activation of a...

  • Triggering of Suicidal Erythrocyte Death by Fascaplysin. Mischitelli, Morena; Jemaà, Mohamed; almasry, Mustafa; Faggio, Caterina; Lang, Florian // Cellular Physiology & Biochemistry (Karger AG);Sep2016, Vol. 39 Issue 4, p1638 

    Background/Aims: The bis-indole alkaloid Fascaplysin is effective against malignancy, an effect at least partially due to stimulation of tumor cell apoptosis. Similar to apoptosis of nucleated cells, erythrocytes could enter suicidal erythrocyte death or eryptosis, characterized by cell...

  • Stimulation of Suicidal Erythrocyte Death by the CDC25 Inhibitor NSC-95397. Jemaà, Mohamed; Mischitelli, Morena; Fezai, Myriam; Almasry, Mustafa; Faggio, Caterina; Lang, Florian // Cellular Physiology & Biochemistry (Karger AG);Dec2016, Vol. 40 Issue 3/4, p597 

    Background/Aims: The CDC25B inhibitor NSC-95397 triggers apoptosis of tumor cells and is thus considered for the treatment of malignancy. The substance is effective in part by modification of gene expression. Similar to apoptosis of nucleated cells erythrocytes may undergo eryptosis, the...

  • Sphingosine but not Sphingosine-1-phosphate Stimulates Suicidal Erythrocyte Death. Qadri, Syed M.; Bauer, Julia; Zelenak, Christine; Mahmud, Hasan; Kucherenko, Yuliya; Lee, Seung Hun; Ferlinz, Klaus; Lang, Florian // Cellular Physiology & Biochemistry (Karger AG);2011, Vol. 28 Issue 2, p339 

    Sphingosine kinase 1 phosphorylates sphingosine, which is converted to ceramide by ceramide synthetase. Ceramide triggers eryptosis, the suicidal erythrocyte death characterized by cell shrinkage and phosphatidylserine (PS) exposure at the erythrocyte surface. Erythrocytes lack sphingosine...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics