Cell-surface expression of Aspergillus saitoi-derived functional α-1,2-mannosidase on Yarrowia lipolytica for glycan remodeling

Moon, Hye; Van, Trinh; Cheon, Seon; Choo, Jinho; Kim, Jeong-Yoon; Kang, Hyun
August 2013
Journal of Microbiology;Aug2013, Vol. 51 Issue 4, p506
Academic Journal
No abstract available.


Related Articles

  • Engineering Yarrowia lipolytica to Produce Glycoproteins Homogeneously Modified with the Universal Man3GlcNAc2 N-Glycan Core. De Pourcq, Karen; Tiels, Petra; Van Hecke, Annelies; Geysens, Steven; Vervecken, Wouter; Callewaert, Nico // PLoS ONE;Jun2012, Vol. 7 Issue 6, p1 

    Yarrowia lipolytica is a dimorphic yeast that efficiently secretes various heterologous proteins and is classified as "generally recognized as safe." Therefore, it is an attractive protein production host. However, yeasts modify glycoproteins with nonhuman high mannose-type N-glycans. These...

  • Engineering the yeast Yarrowia lipolytica for the production of therapeutic proteins homogeneously glycosylated with Man8GlcNAc2 and Man5GlcNAc2. De Pourcq, Karen; Vervecken, Wouter; Dewerte, Isabelle; Valevska, Albena; Van Hecke, Annelies; Callewaert, Nico // Microbial Cell Factories;2012, Vol. 11 Issue 1, p53 

    Background: Protein-based therapeutics represent the fastest growing class of compounds in the pharmaceutical industry. This has created an increasing demand for powerful expression systems. Yeast systems are widely used, convenient and cost-effective. Yarrowia lipolytica is a suitable host that...

  • Class IIC {alpha}-mannosidase AfAms1 is required for morphogenesis and cellular function in Aspergillus fumigatus. Yanjie Li; Wenxia Fang; Lei Zhang; Haomiao Ouyang; Hui Zhou; Yuanming Luo; Cheng Jin // Glycobiology;Jun2009, Vol. 19 Issue 6, p624 

    The mammalian ER/cytosolic α-mannosidase (Man2C1p), yeast vacuolar α-mannosidase (Ams1p) and the Aspergillus nidulans α-mannosidase are members of Class IIC subgroup, which is involved in oligosaccharide catabolism and N-glycan processing. Unlike their mammalian counterparts, the yeast...

  • Heterologous expression and characterization of a β-1,6-glucanase from Aspergillus fumigatus. Boisramé, A.; Gaillardin, C. // Applied Microbiology & Biotechnology;Mar2009, Vol. 82 Issue 4, p663 

    The cell wall of Candida albicans is composed of mannoproteins associated to glycan polymers. Most of these proteins are retained in this compartment through a phosphodiester linkage between a remnant of their glycosylphosphatidylinositol anchor and the β-1,6-glucan polymer. A pure...

  • Engineering towards a complete heterologous cellulase secretome in Yarrowia lipolytica reveals its potential for consolidated bioprocessing. Hui Wei; Wei Wang; Alahuhta, Markus; Wall, Todd Vander; Baker, John O.; Taylor II, Larry E.; Decker, Stephen R.; Himmel, Michael E.; Zhang, Min // Biotechnology for Biofuels;2014, Vol. 7 Issue 1, p1 

    Background Yarrowia lipolytica is an oleaginous yeast capable of metabolizing glucose to lipids, which then accumulate intracellularly. However, it lacks the suite of cellulolytic enzymes required to break down biomass cellulose and cannot therefore utilize biomass directly as a carbon source....

  • Awakening the endogenous Leloir pathway for efficient galactose utilization by Yarrowia lipolytica. Lazar, Zbigniew; Gamboa-Meléndez, Heber; Crutz-Le Coq, Anne-Marie; Neuvéglise, Cécile; Nicaud, Jean-Marc // Biotechnology for Biofuels;11/25/2015, Vol. 8, p1 

    Background: Production of valuable metabolites by Yarrowia lipolytica using renewable raw materials is of major interest for sustainable food and energy. Galactose is a monosaccharide found in galactomannans, hemicelluloses, gums, and pectins. Results: Yarrowia lipolytica was found to express...

  • Environmental and industrial applications of Yarrowia lipolytica. Bankar, Ashok V.; Kumar, Ameeta R.; Zinjarde, Smita S. // Applied Microbiology & Biotechnology;Oct2009, Vol. 84 Issue 5, p847 

    Yarrowia lipolytica is a fungus that degrades hydrophobic substrates very efficiently. The fungus displays several important characteristics that have encouraged researchers to study various basic biological and biotechnological applications in detail. Although the organism has been used as...

  • Chemical remodelling of cell surfaces in living animals. Prescher, Jennifer A.; Dube, Danielle H.; Bertozzi, Carolyn R. // Nature;8/19/2004, Vol. 430 Issue 7002, p873 

    Cell surfaces are endowed with biological functionality designed to mediate extracellular communication. The cell-surface repertoire can be expanded to include abiotic functionality through the biosynthetic introduction of unnatural sugars into cellular glycans, a process termed metabolic...

  • Characterization of putative glycosylphosphatidylinositol-anchoring motifs for surface display in the methylotrophic yeast Hansenula polymorpha. Cheon, Seon; Jung, Jinhee; Choo, Jin; Oh, Doo-Byoung; Kang, Hyun // Biotechnology Letters;Oct2014, Vol. 36 Issue 10, p2085 

    Bioinformatic analysis of the genome of the methylotrophic yeast Hansenula polymorpha revealed 39 putative glycosylphosphatidylinositol-anchored proteins (GPI-proteins). Notably, dibasic motifs in the proximal ω-site, that has been reported as a plasma membrane retention signal in...

  • Global metabolic inhibitors of sialyl- and fucosyltransferases remodel the glycome. Rillahan, Cory D; Antonopoulos, Aristotelis; Lefort, Craig T; Sonon, Roberto; Azadi, Parastoo; Ley, Klaus; Dell, Anne; Haslam, Stuart M; Paulson, James C // Nature Chemical Biology;Jul2012, Vol. 8 Issue 7, p661 

    Despite the fundamental roles of sialyl- and fucosyltransferases in mammalian physiology, there are few pharmacological tools to manipulate their function in a cellular setting. Although fluorinated analogs of the donor substrates are well-established transition state inhibitors of these...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics