Circulating cell-free DNA in serum as a biomarker of colorectal cancer

Da Silva Filho, Benisio Ferreira; Diniz Gurgel, Ana Pavla Almeida; Freitas Lins Neto, Manoel Álvaro de; De Azevedo, Dalmo Almeida; De Freitas, Antonio Carlos; Silva Neto, Jacinto da Costa; Ferreira Silva, Luiz Antonio
September 2013
Journal of Clinical Pathology;Sep2013, Vol. 66 Issue 9, p775
Academic Journal
Aims The aim of this study was to report circulating cell-free DNA using ALU247 and ALU247/ALU115 biomarkers in serum of operated and non-operated patients with colorectal cancer (CRC). Methods To undertake this, 90 blood samples were collected, including 30 samples from healthy volunteers; 27 samples from CRC non-operated patients and 33 samples from CRC-operated patients. Circulating cell-free DNA was verified through quantitative real-time PCR (qPCR) using ALU115 and ALU247 primers. Results With regard to the ALU115-qPCR biomarker, the increased levels of circulating cell-free DNA in serum of non-operated patients were significant when compared with control ( p<0.05). Moreover, levels of ALU247-qPCR biomarker were statistically significant between non-operated versus operated and nonoperated versus control groups ( p=0.000). With regard to the ALU247/115-qPCR biomarker, significant differences were observed between control versus non-operated patients ( p=0.019), operated versus nonoperated patients (p=0.005) and control versus operated patients ( p=0.043). Conclusions Thus, the ALU247 and ALU247/ ALU115-qPCR biomarkers may be important in detecting and monitoring CRC patients in both early and late stages


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