Dichlorophen and Dichlorovos mediated genotoxic and cytotoxic assessment on root meristem cells of Allium cepa

Shaikh, Sibhghatulla; Nazam, Nazia; Lone, Mohammad Iqbal; Ahmad, Waseem
January 2012
Science Diliman;Jan-Jun2012, Vol. 24 Issue 1, p13
Academic Journal
Plants are direct recipients of agro - toxics and therefore important materials for assessing environmental chemicals for genotoxicity. The meristematic mitotic cell of Allium cepa is an efficient cytogenetic material for chromosome aberration assay on environmental pollutants. Onion root tips were grown on moistened filter paper in petri dish at room temperature. Germinated root tips were then exposed to three concentrations of each pesticide for 24 h. About 1 - 2 mm length of root tip was cut, fixed in cornoy's fixative, hydrolyzed in warm 1 N HCL, stained with acetocarmine and squashed on glass slide. About 3000 cells were scored and classified into interphase and normal or aberrant division stage. Cytotoxicity was determined by comparing the mitotic index (MI) of treated cells with that of the negative control. The MI of cells treated with Dichlorophen and Dichlorovos at one or more concentration was half or less than that of control are said to be cytotoxic. Genotoxicity was measured by comparing the number of cells/1000 in aberrant division stages at each dose with the negative control using Mann - Whitney U test. Both Dichlorophen and Dichlorovos are genotoxic at higher concentrations i.e. 0.001%, 0.002% and 0.028%, 0.056% inducing chromosome fragment, chromosome lagging and bridges, stick chromosome and multipolar anaphase.


Related Articles

  • Studies on the Cytotoxic Effect of Oil Refinery Sludge on Root Meristem. Tanti, Bhaben; Buragohain, Alak K.; Dutta, Snehashish; Gurung, Lisha; Shastry, Madhu; Borah, Sailendra Prasad // Advances in Environmental Biology;Jan-Apr2009, Vol. 3 Issue 1, p10 

    Cytotoxicity of the oil refinery sludge was studied using Allium cepa L. as a test plant. Onion roots were treated with different concentrations of pollutant for different durations along with controls. The high mitotic index and lower percentage of abnormality was very much evident in roots...

  • Cytotoxic and Genotoxic Effects of Diphenyl-ether Herbicide GOAL (Oxyfluorfen) using the Allium cepa test. Dragoeva, Asya; Koleva, Vanya; Hasanova, Nurzhihan; Slanev, Stoicho // Research Journal of Mutagenesis;2012, Vol. 2 Issue 1, p1 

    The pesticides possess biological activity including genotoxic influence and can influence non target organisms. Oxyfluorfen (2-chloro-1-(3-ethoxy-4-nitrophenoxy)-4-(trifluoromethyl) benzene) is classified as a possible human carcinogen. In present study cytotoxic and genotoxic effects of...

  • Cytotoxic and Genotoxic Effects of cis-Tetraammine(oxalato)Ruthenium(III) Dithionate on the Root Meristem Cells of Allium cepa. Flávia de Pereira; Cesar Vilanova-Costa; Aliny Lima; Alessandra de Ribeiro; Hugo Silva; Luiz Pavanin; Elisângela de Silveira-Lacerda // Biological Trace Element Research;Jun2009, Vol. 128 Issue 3, p258 

    Abstract  Ruthenium complexes have attracted much attention as possible building blocks for new transition-metal-based antitumor agents. The present study examines the mitotoxic and clastogenic effects induced in the root tips of Allium cepa by cis-tetraammine(oxalato)ruthenium(III)...

  • Exposure of Allium cepa Root Cells to Zidovudine or Nevirapine Induces Cytogenotoxic Changes. Onwuamah, Chika K.; Ekama, Sabdat O.; Audu, Rosemary A.; Ezechi, Oliver C.; Poirier, Miriam C.; Odeigah, Peter G C. // PLoS ONE;Mar2014, Vol. 9 Issue 3, p1 

    Antiretroviral drugs have proved useful in the clinical management of HIV-infected persons, though there are concerns about the effects of exposure to these DNA-reactive drugs. We investigated the potential of the plant model Allium cepa root tip assay to demonstrate the cytogenotoxicity of...

  • Cytotoxicity and Genotoxicity Potential of Thiocyclam in Root-Tip Cells of Allium cepa. Sarhan, Mohammed A. A. // International Journal of Biotechnology & Biochemistry;Aug2010, Vol. 6 Issue 4, p601 

    Currently, Allium cepa as ahigher plant gain great interest as alternatives to mammals in monitoring pollution in the environment because of their direct recipients of agrotoxics. In this study, five doses of thiocyclam, (0.073, 0.146, 0.29, 0.59, 1.17 g/ml) solution were assessed for...

  • Toxicological Evaluation of Root Methanolic Extract of Strobilanthes heyneanus Nees Using Allium Test. Renjana, P. K; Thoppil, John E. // International Journal of Pharmaceutical Sciences & Drug Research;Jul-Sep2013, Vol. 5 Issue 3, p125 

    Cytotoxic and genotoxic effects of methanolic extract of the roots of Strobilanthes heyneanus Nees have been studied on the root meristem cells of Allium cepa. Roots of A. cepa were exposed to different concentrations (0.01%, 0.05%, 0.1% and 0.5%) of the extract for ½, 1, 2 and 3 h. Mitotic...

  • Cytotoxicity and genotoxicity of versicolorins and 5-methoxysterigmatocystin in A549 cells. Jakšić, Daniela; Puel, Olivier; Canlet, Cécile; Kopjar, Nevenka; Kosalec, Ivan; Klarić, Maja // Archives of Toxicology;Oct2012, Vol. 86 Issue 10, p1583 

    Aspergillus versicolor and A. flavus are primary colonizers in damp dwellings, and they produce sterigmatocystin (ST) and aflatoxin B1 (AFB), respectively. These hepatotoxic and carcinogenic mycotoxins and their precursors and derivates possess a furofuran ring, which has proven responsible for...

  • Comparative Study of the Cytotoxicity and Genotoxicity of Alpha- and Beta-Asarone. Unger, Pascal; Melzig, Matthias F. // Scientia Pharmaceutica;2012, Vol. 80 Issue 3, p663 

    The cytotoxicity of alpha- and beta-asarone was investigated with the BrdU assay in HepG2-cells. Alpha-asarone was found to be more toxic than beta-asarone after 24 hours of treatment. Investigation of the genotoxicity using the micronucleus assay in the HepG2-cell system showed that only after...

  • Pirimicarb-based formulation-induced genotoxicity and cytotoxicity in the freshwater fish Cnesterodon decemmaculatus (Jenyns, 1842) (Pisces, Poeciliidae). Vera-Candioti, Josefina; Soloneski, Sonia; Larramendy, Marcelo L. // Toxicology & Industrial Health;Nov2015, Vol. 31 Issue 11, p1051 

    We analyzed the aspects of lethality, genotoxicity, and cytotoxicity in the ten spotted live-bearer exposed under laboratory conditions to the pirimicarb-based formulation Patton Flow® (50% active ingredient (a.i.)). Acute effects were evaluated using different end points for lethality,...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics