TITLE

PDEA-coated magnetic nanoparticles for gene delivery to Hep G2 cells

AUTHOR(S)
Sun, Hanwen; Zhu, Xinjun; Zhang, Lianying; Gu, Xiangling; Wang, Jinghe; Li, Jing; Zhang, Yancong
PUB. DATE
July 2013
SOURCE
Biotechnology & Bioprocess Engineering;Jul2013, Vol. 18 Issue 4, p648
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Poly(2-(diethylamino)ethyl methacrylate) coated magnetic nanoparticles (PDEA-MNPs) were synthesized as a new gene nanocarrier to delivery plasmids (pEGFPN1 and pRL-TK) into human hepatoma (Hep G2) cells. The PDEA-MNPs shows the pH-sensitive property. These nanoparticles are positively charged at acidic pH and negatively charged at neutral or alkaline pH. The PDEAMNPs exhibited a low cytotoxicity in Hep G2 cells. PDEA-MNPs could bind and protect DNA from DNase I degradation. The transfection study demonstrated that the PDEA-MNPs could carry plasmid into Hep G2 cells and exhibited a high gene transfection efficiency. These results indicated that the novel magnetic nanoparticles could enhance gene transfection in vitro and hold the potential to be a promising non-viral nanodevice.
ACCESSION #
89701862

 

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