TITLE

IN VITRO EFFECTIVENESS OF PINUS LONGIFOLIA ON CANDIDA SPECIES ISOLATED FROM PATIENTS WITH VULVOVAGINAL CANDIDIASIS IN ISFAHAN

AUTHOR(S)
Norouz, Zohreh; Madani, Mahboobeh; Amjad, Leila
PUB. DATE
July 2013
SOURCE
Jundishapur Journal of Microbiology;2013 Special Edition, p14
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Introduction and Objectives: Vulvovaginal candidiasis is a serious public health problem in women that caused by Candida species specially Candida albicans. The plant products and their active constituents play an important role in maintaining perfect health. The present investigation was an attempt to evaluate the in vitro effectiveness of female cones of Pinus longifolia extract on the growth of Candida species isolated from patients with vulvovaginal candidiasis in Isfahan. Materials and Methods: Clinical isolates of Candida were evaluated with mycological algorithms, including morphology on corn meal ager and CHROMagar, germ tube production and biochemical methods. Female cones of P. longifolia were extracted by Soxhlet apparatus using ethanol and water as solvents. The anticandidal properties were evaluated using the agar well diffusion method. Determination of minimum inhibitory concentration was performed using broth microdilution assay. Fluconazole was used as positive control. Results: Four species of Candida were isolated from patients and recognized as Candida albicans, C. parapsilosis, C. glabrata and C. tropicalis. Maximum inhibition zone of ethnologic and aqueous extracts of P. longifolia were 16, 14, 15, 12 and 15, 13, 14, 11 mm and the minimum inhibitory concentration were 5,5,10,10 and 5, 5,10, 20 mg/ml for above-mentioned species, respectively. Conclusion: The results of this work showed antifungal activity of ethnologic and aqueous extracts of female cones of P. longifolia on Candida species.
ACCESSION #
89588590

 

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