Isolation and characterization of four alternate c-erbB3 transcripts expressed in ovarian carcinoma-derived cell lines and normal human tissues

Lee, Hakjoo; Maihle, Nita J
June 1998
Oncogene;6/25/98, Vol. 16 Issue 25, p3243
Academic Journal
ErbB-3 is a member of the epidermal growth factor receptor (EGFR/ErbB) family. In addition to the previously reported 6.2 kb full-length and 1.4 kb truncated c-erbB3 transcripts, we have observed a 1.7 kb c-erbB3 human transcript in Northern blots that specifically hybridizes to a probe of the extracellular domain of the receptor. Using 3′-RACE we have isolated four novel c-erbB3 cDNA clones of 1.6, 1.7, 2.1 and 2.3 kb from a human ovarian carcinoma-derived cell line. All four alternate transcripts are synthesized by readthrough of an intron and use of an alternative polyadenylation signal within this intron. Identical c-erbB3 transcripts are expressed in normal human placental tissues. Expression of these alternate transcripts is tissue-specific as indicated by Northern blot and RNase protection analyses. Fibroblasts transfected with expression vectors carrying these alternate c-erbB3 cDNA clones stably express truncated ErbB-3 products. Three of these four cDNA clones express a receptor product that is secreted. Immunoprecipitation analysis of primary cultures of human ovarian carcinomas also demonstrate the expression of a 90 kDa ErbB-3 related protein that is secreted. Furthermore, we demonstrate conservation of the exon-intron junctions between members of the erbB gene family in those regions of the gene encoding the extracellular domain. This gene structure is also conserved in the c-erbB1 homologues of Drosophila and C. elegans. Growth regulatory roles for related truncated ErbB products recently have been reported. It is, therefore, possible that the products of these four alternate c-erbB3 transcripts may also play important growth regulatory roles in normal and transformed cells.


Related Articles

  • Selection and validation of endogenous reference genes using a high throughput approach. Ping Jin; Yingdong Zhao; Ngalame, Yvonne; Panelli, Monica C.; Nagorsen, Dirk; Monsurró, Vladia; Smith, Kina; Nan Hu; Hua Su; Taylor, Phil R.; Marincola, Francesco M.; Ena Wang // BMC Genomics;2004, Vol. 5, p55 

    Background: Endogenous reference genes are commonly used to normalize expression levels of other genes with the assumption that the expression of the former is constant in different tissues and in different physiopathological conditions. Whether this assumption is correct it is, however, still...

  • A novel method of differential gene expression analysis using multiple cDNA libraries applied to the identification of tumour endothelial genes. Herbert, John M. J.; Stekel, Dov; Sanderson, Sharon; Heath, Victoria L.; Bicknell, Roy // BMC Genomics;2008, Vol. 9, Special section p1 

    Background: In this study, differential gene expression analysis using complementary DNA (cDNA) libraries has been improved. Firstly by the introduction of an accurate method of assigning Expressed Sequence Tags (ESTs) to genes and secondly, by using a novel likelihood ratio statistical scoring...

  • Expression, purification and characterization of rat angiopoietin-2 in Pichia pastoris. Wang, Jinfeng; Zhou, Leilei; Cai, Xin; Zou, Minji; Wang, Yuanyuan; Fu, Wenliang; Wang, Jiaxi; Xu, Donggang // Molecular Biology Reports;Dec2010, Vol. 37 Issue 8, p3909 

    ngiopoietin-2 (Ang2) is a member of the Ang family. Its potential in clinical use has been highlighted for its important roles in angiogenesis during the individual development and the growth of tumors. Ang2 is difficult to be expressed in E. coli for its unique structure. The expressions of...

  • Isolation of growth suppressors from a cDNA expression library. Pestov, Dimitri G; Grzeszkiewicz, Tatiana M; Lau, Lester F // Oncogene;12/17/98, Vol. 17 Issue 24, p3187 

    We describe an experimental procedure for the isolation of growth inhibitory sequences from a complex cDNA library. This approach first takes advantage of the SETGAP technique (selectable expression of transient growth arrest phenotype) to enrich for growth inhibitory sequences, followed by a...

  • Global analysis of differential gene expression between prostate cancer and normal prostate tissues using cDNA microarray of open-reading frame expressed sequence tags (ORESTES). Sarkis, A.S. // Prostate Cancer & Prostatic Diseases;2000 Supplement 1, Vol. 3 Issue 4, pS36 

    Presents an analysis of differential gene expression between prostate cancer and normal prostate tissues using complementary DNA microarray of open-reading frame expressed sequence tags (ORESTES). Results of sequencing of ORESTES fragments; Approximate number of available ORESTES.

  • Woodchuck post-transcriptional element induces nuclear export of myotonic dystrophy 3'untranslated region transcripts. Mastroyiannopoulos, Nikolaos P.; Feldman, Mariana L.; Uney, James B.; Mahadevan, Mani S.; Phylactou, Leonidas A. // EMBO Reports;May2005, Vol. 6 Issue 5, p458 

    The woodchuck post-transcriptional regulatory element (WPRE) can naturally accumulate hepatitis transcripts in the cytoplasm, and has been recently exploited as an enhancer of transgene expression. The retention of mutant myotonic dystrophy protein kinase (DMPK) transcripts in the nucleus of...

  • Increased susceptibility to apoptosis in CD45+ myeloma cells accompanied by the increased expression of VDAC1. Liu, S.; Ishikawa, H.; Tsuyama, N.; Li, F. -J.; Abroun, S.; Otsuyama, K. -i.; Zheng, X.; Ma, Z.; Maki, Y.; Iqbal, M. S.; Obata, M; Kawano, M. M. // Oncogene;1/19/2006, Vol. 25 Issue 3, p419 

    Expression of CD45 is quite variable in human myeloma cells and cell lines, such as U266, and CD45+ U266 proliferates in response to a growth factor, interleukin-6. Here, we show that CD45+ myeloma cell lines were more sensitive to various apoptotic stimuli, such as oxidative stress and...

  • Transcriptional Changes Caused by Bisphenol A in Oryzias javanicus, a Fish Species Highly Adaptable to Environmental Salinity. Seonock Woo; Denis, Vianney; Seungshic Yum // Marine Drugs;Feb2014, Vol. 12 Issue 2, p983 

    The Javanese medaka, Oryzias javanicus, is a fish highly adaptable to various environmental salinities. Here, we investigated the effects of the environmental pollutant bisphenol A (BPA; an endocrine disrupting chemical) on gene expression levels in this species acclimated to different...

  • Cloning and Functional Studies of a Splice Variant of CYP261 Expressed in Vascular Cells. Elmabsout, Ali Ateia; Kumawat, Ashok; Saenz-Méndez, Patricia; Krivospitskaya, Olesya; Sävenstrand, Helena; Olofsson, Peder S.; Eriksson, Leif A.; Strid, Åke; Valen, Guro; Törmä, Hans; Sirsjö, Allan // PLoS ONE;May2012, Vol. 7 Issue 5, p1 

    Background: All-trans retinoic acid (atRA) plays an essential role in the regulation of gene expression, cell growth and differentiation and is also important for normal cardiovascular development but may in turn be involved in cardiovascular diseases, i.e. atherosclerosis and restenosis. The...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics