TITLE

Use of shift gradient in the second dimension to improve the separation space in comprehensive two-dimensional liquid chromatography

AUTHOR(S)
Li, Duxin; Schmitz, Oliver
PUB. DATE
August 2013
SOURCE
Analytical & Bioanalytical Chemistry;Aug2013, Vol. 405 Issue 20, p6511
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Comprehensive two-dimensional liquid chromatography (LC × LC) has received much attention because it offers much higher peak capacities than separation in a single dimension. The advantageous peak capacity makes it attractive for the separation of complex samples. Various gradient methods have been used in LC × LC systems. The use of continuous shift gradient is advantageous because it combines the peak compression effect of full gradient mode and the tailed gradient program in parallel gradient mode. Here, a comparison of LC × LC analysis of Chinese herbal medicine with full gradient mode and shift gradient mode in the second dimension was performed. A correlation between the first and second dimensions was found in full gradient mode, and this was significantly reduced with shift gradient mode. The orthogonality increased by 43.7 %. The effective peak distribution area increased significantly, which produced better separation.
ACCESSION #
89077486

 

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