TITLE

Definite differentiation of Candida Albicans from other species by using chrom agar

AUTHOR(S)
Reddy, P. Srinivasulu; Athuluri, Vinod
PUB. DATE
November 2012
SOURCE
Journal of Microbiology & Biotechnology Research;2012, Vol. 2 Issue 6, p936
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Differences in the expression of putative virulence factors and in antifungal susceptibility among different Candida species has raised the need for species-level identification of candida. The close relationship of Candida dubliniensis with C. albicans has led to misidentification. Thus, the aim of the present study was to differentiate C.albicans among the various clinical isolates of Candida species. A total of 886 isolates from various clinical samples were screened for candida species and these were streaked simultaneously on CHROM agar, Pal's agar and a combination of CHROM agar supplemented with Pal's agar for identification. Out of 886 isolates after 48 hours of incubation on CHROM agar 75 isolates were identified as C. albicans produced green colonies. On Pal's agar and CHROM agar supplemented with Pal's agar, all 75 isolates of C. albicans failed to exibit hyphal fringe. In additional test also C. albicans isolates grows at 450C and failed to reduce TTC dye. CHROM agar and CHROM agar supplemented with Pal's agar gave definitive differentiation of C. albicans from the yeast cultures. CHROM agar supplemented Pal's agar makes rapid differentiation and can be easily incorporated in routine use.
ACCESSION #
88924720

 

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