TITLE

In vitro Propagation of Ochlandra wightii (Munro) Fisch.: An Endemic Reed of Southern Western Ghats India

AUTHOR(S)
Bejoy, M.; Amsh, N. P.; Radhika, B. J.; Nair, G. M.
PUB. DATE
May 2012
SOURCE
Biotechnology;2012, Vol. 11 Issue 2, p67
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Ochlandra wightii (Munro) Fisch., an endemic reed bamboo in the Southern Western Ghats has been multiplied using tissue culture techniques. In vitro production of O. wightii plantlets has been achieved by using isolated embryos from mature seeds and nodal segments from in vitro seedlings. Better germination of isolated embryos (85%) was noticed in BAP (6-benzyl amino purine) (0.5 mg L-1) with 1-2 shoots in 30 days. Half-strength MS medium supplemented with BAP (0.5 mg L-1) and TDZ (Thidiazuron) (0.5 mg L-1) enhanced the rate of shoot multiplication to an average of 9.8 shoots in 60 days. Simultaneous development of roots along with shoots was also noticed. Rhizome induction was achieved from 65% of seedling cultures when treated with KN (Kinetin) (1 mg L-1) and higher concentration of sucrose (4%) in 35 days. Multiple shoots were also induced from nodal explants obtained from in vitro seedlings. Average 3.6 shoots were achieved in 40 days when treated with BAP (2 mg L-1) and KN (0.5 mg L-1). Twenty percent of the nodal cultures developed 1 -2 roots during shoot development and the rest were rooted in half-strength MS solid medium supplemented with IB A (indole-3-buryric acid) (0.5 mg L-1). Plantlets with healthy roots were hardened and transplanted to the clay pots. They showed 80% survival after 6 months. However, the plantlets with in vitro rhizomes showed 100% survival. Development of new culms and rhizomes were noticed after 3 months of greenhouse growth. Around 880 plantlets could be generated in 9 months from 10 embryos isolated from mature seeds using this protocol.
ACCESSION #
88913676

 

Related Articles

  • Effective Sterilization Protocol for Micropropagation of Musa coccinea (Musa SPP). Farzinebrahimi, Reza; Rashid, Kamaludin; Taha, Rosna Mat; Yaacob, Jamilah Syafawati // International Proceedings of Chemical, Biological & Environmenta;2013, Vol. 60, p118 

    In order to reduce contamination on tissue culture of Musa family and investigate of different methods of explant sterilization, three methods of aseptic techniques on Musa coccinea using male bud and sucker explants were carried out. Using of 100% Clorox for 6 minutes and two times soaking and...

  • THE USE OF IN VITRO CULTURE IN DIANTHUS PROPAGATION. Marković, Marija; Grbić, Mihailo; Đukić, Matilda // Bulletin of the Faculty of Forestry / Glasnik Sumarskog fakultet;2013, Vol. 107, p141 

    Today, in vitro culture is of the great importance in both scientific investigation of under-researched plant species and plant production. In this paper, a review of development and methods of in vitro culture is presented. The main principles are given and the most commonly used methods are...

  • Mesos components (CaCl, MgSO, KHPO) are critical for improving pear micropropagation. Wada, Sugae; Niedz, Randall; DeNoma, Jeanine; Reed, Barbara // In Vitro Cellular & Developmental Biology Plant;Jun2013, Vol. 49 Issue 3, p356 

    Pear accessions and species show a broad response to tissue culture media due to the wide genetic diversity that exists in the available pear germplasm. An initial study of mineral nutrition using a systematic response surface approach with five Murashige and Skoog medium mineral stock solutions...

  • Researches Concerning Improving Methods of In Vitro Microtubers Production. NISTOR, Andreea; CHIRU, Nicoleta; CIOLOCA, Mihaela; POPA, Monica // Bulletin of the University of Agricultural Sciences & Veterinary;2014, Vol. 71 Issue 1, p118 

    Five genotypes of potato (Solanum tuberosum L.) were induced to form microtubers under two in vitro culture conditions (continuos darkness and light). Cultures maintained under continuos darkness (with a temperatue of 17°C) had a higher yield with a greater number of...

  • Hormonal signals involved in the regulation of cambial activity, xylogenesis and vessel patterning in trees. Sorce, Carlo; Giovannelli, Alessio; Sebastiani, Luca; Anfodillo, Tommaso // Plant Cell Reports;Jun2013, Vol. 32 Issue 6, p885 

    The radial growth of plant stem is based on the development of cribro-vascular cambium tissues. It affects the transport efficiency of water, mineral nutrients and photoassimilates and, ultimately, also plant height. The rate of cambial cell divisions for the assembly of new xylem and phloem...

  • Structural basis for cytokinin receptor signaling: an evolutionary approach. Steklov, Mikhail; Lomin, Sergey; Osolodkin, Dmitry; Romanov, Georgy // Plant Cell Reports;Jun2013, Vol. 32 Issue 6, p781 

    Cytokinins are ubiquitous plant hormones; their signal is perceived by sensor histidine kinases-cytokinin receptors. This review focuses on recent advances on cytokinin receptor structure, in particular sensing module and adjacent domains which play an important role in hormone recognition,...

  • Cytokinins résumé: their signaling and role in programmed cell death in plants. Kunikowska, A.; Byczkowska, A.; Doniak, M.; Kaźmierczak, A. // Plant Cell Reports;Jun2013, Vol. 32 Issue 6, p771 

    Cytokinins (CKs) are a large group of plant hormones which play a crucial role in many physiological processes in plants. One of the interesting functions of CKs is the control of programmed cell death (PCD). It seems that all CKs-dependent phenomena including PCD are accompanied by special...

  • Usual and unusual development of the dicot leaf: involvement of transcription factors and hormones. Fambrini, Marco; Pugliesi, Claudio // Plant Cell Reports;Jun2013, Vol. 32 Issue 6, p899 

    Morphological diversity exhibited by higher plants is essentially related to the tremendous variation of leaf shape. With few exceptions, leaf primordia are initiated postembryonically at the flanks of a group of undifferentiated and proliferative cells within the shoot apical meristem (SAM) in...

  • Expression, purification, and characterization of cytokinin signaling intermediates: Arabidopsis histidine phosphotransfer protein 1 (AHP1) and AHP2. Verma, Vivek; Sivaraman, J.; Kumar, Prakash // Plant Cell Reports;Jun2013, Vol. 32 Issue 6, p795 

    Key message: We have expressed, purified, and biophysically characterized recombinant AHP1 and AHP2. Also, using computational homology models for AHP1, ARR7, and AHP1-ARR7 complex, we identified three-dimensional positioning of key amino acids. Abstract: Cytokinin signaling involves activation...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics