Total phenolic contents and free-radical scavenging activities from methanolic extracts of Nauclea subdita (Korth) Steud. heartwood

Jamaluddin, Fatin Ruzanna; Wahab, Razak; Daud, Jamaluddin M.; Rahman, Shafiqur
July 2012
Advances in Natural & Applied Sciences;2012, Vol. 6 Issue 7, p1116
Academic Journal
Investigation on the total phenolic content (TPC) and free-radical scavenging activity of Nauclea subdita (Korth) Steud. were carried out. Information on these activities might reflect the potency of the plant extract as an antioxidant. Young and matured trees of N. subdita were harvested and cut into three heartwood parts (top, middle and bottom) and grounded into powdered forms. Soxhlet extraction of the heartwood samples using polar (methanol) and non-polar (hexane) solvents, and phytochemical screening tests were carried out. The total phenolic contents of the methanolic crude extracts from heartwood parts were determined by colorimetry at 760 nm using Folin-Ciocalteau's reagent, and gallic acid was used as standard. The methanolic extracts were also checked for primary antioxidant activities using an in vitro method, measured by free-radical scavenging activity of 2, 2'-diphenyl-1- picrylhydrazyl (DPPH) radical. The absorbances were measured at 517 nm by UV/VIS spectrophotometer and compared with standards Trolox and butylated hydroxyanisole (BHA). The results from this study suggest that N. subdita heartwood extract may serve as a potential source of natural antioxidant for future development in food and nutraceutical applications.


Related Articles

  • Evaluation of Antioxidant Capacity of Two Ocimum Species Consumed Locally as Spices in Nigeria as a Justification for Increased Domestication. Uyoh, Edak A.; Chukwurah, Peter N.; David, Imabasi A.; Bassey, Anna C. // American Journal of Plant Sciences;Feb2013, Vol. 4 Issue 2, p221 

    The antioxidant activities of Ocimum basilicum and Ocimum gratissimum consumed as spices in Nigeria were evaluated in a bid to provide better scientific basis for increased domestication efforts on them. Total phenol and flavonoid contents of the spice extracts ranged from 9.09 - 27.41 µg...

  • Application of Electron Paramagnetic Resonance Spectroscopy for Investigating Antioxidant Activity of Selected Herbs. BARTOSZEK, MARIOLA; POLAK, JUSTYNA // Journal of AOAC International;Jul/Aug2015, Vol. 98 Issue 4, p862 

    Antioxidant properties of selected herbs were investigated using electron paramagnetic resonance (EPR) spectroscopy. This was possible by measuring changes in the intensity of the EPR spectrum that resulted from the interaction of the stable radical 1,1-diphenyl-2-picrylhydrazyl with...

  • Quantification of Gallic Acid and Tannic Acid from Quercus infectoria (Manjakani) and their Effects on Antioxidant and Antibacterial Activities. Ab. Rahman, N. S.; L., Md. Salleh; A., Abd. Majid F.; Harisun, Y. // Pertanika Journal of Science & Technology;Jul2015, Vol. 23 Issue 2, p351 

    Effects of different types of solvent on the antioxidant and antibacterial activity of Quercus infectoria extract have not been well documented. Therefore, extraction process was conducted using conventional Soxhlet extraction with six different types of solvent (100% methanol, ethanol, acetone,...

  • COMPOSTOS FENÓLICOS E ATIVIDADE ANTIOXIDANTE DE GENÓTIPOS DE CIRIGUELAS (SPONDIA PURPUREA L.). da SILVA, Quésia Jemima; MOREIRA, Aldenise Chagas Curvêlo Gonçalves; de Almeida MELO, Enayde; de LIMA, Vera Lúcia Arroxelas Galvão // Brazilian Journal of Food & Nutrition / Alimentos e Nutrição;Jan-Mar2012, Vol. 23 Issue 1, p73 

    The aim of this research was to evaluate the antioxidant potential of red mombin fruits (Spondias purpurea L.) from 11 genotypes. Extracts were obtained by sequential extraction using methanol (80%) and acetone (80%), and subjected to quantification total phenolics content and antioxidant...

  • Production of Antioxidants by Marasmiellus sp. via Solid Substrate Fermentation. Daker, M.; Abdullah, N.; Vikineswary, S.; Kuppusamy, U. R. // American Journal of Food Technology;2009, Vol. 4 Issue 1, p36 

    This study was aimed to evaluate the antioxidant properties of methanol extracts of fermented substrates optimised for antioxidant production by Marasmiellus sp. KUM 50061 mycelial biomass. Extract of fermented maize supplemented with (w/w) malt extract 4%, yeast extract 4% and rice bran 4%...

  • Assessment of the Content of Phenolics and Antioxidant Action of Inflorescences and Leaves of Selected Species from the Genus Sorbus Sensu Stricto. Olszewska, Monika A.; Nowak, Sławomira; Michel, Piotr; Banaszczak, Piotr; Kicel, Agnieszka // Molecules;Dec2010, Vol. 15 Issue 12, p8769 

    In order to find new sources of natural antioxidants, the antioxidant potential of 70% methanolic extracts from the inflorescences and leaves of 16 species from the genus Sorbus s.s. was evaluated using two complementary in vitro test systems: the DPPH (2,2-diphenyl-1-picrylhydrazyl)...

  • Evaluation of Antioxidant Activity, Phytochemicals and ESR Analysis of Lavandula Stoechas. CEYLAN, Y.; USTA, K.; USTA, A.; MALTAS, E.; YILDIZ, S. // Acta Physica Polonica, A.;Aug2015, Vol. 128 Issue 2B, pB-483 

    The antioxidant activity of phytochemicals and of the methanolic extract of Lavandula stoechas was evaluated by β-carotene-linoleate bleaching, DPPH radical scavenging, ferric reducing/antioxidant power (FRAP). The obtained results were compared with antioxidant activities of synthetic...

  • HPTLC determination of Gallic acid in Methanol extract of Quercus griffithii Acorn. Tsering, Jambey; Gogoi, Baikuntha J.; Veer, Vijay; Kalita, Pallabi; Tag, Hui // International Journal of PharmTech Research;Aug/Sep2014, Vol. 6 Issue 4, p1341 

    This paper describes HPTLC determination of gallic acid in methanol extract of Q. griffithii acorn. Macerated extract solution of Q. griffithii acorn was applied on HPTLC plate along with standard by using Camag Linomat-5. The detection of gallic acid was performed on aluminium plates pre-coated...

  • Simultaneous Determination of Four Phenolic Acids in Terminalia by HPLC. XIAOFEN LI; GONGXIN ZHANG; XUEJIN ZHOU; YUNTAO GAO // Asian Journal of Chemistry;2012, Vol. 24 Issue 6, p2585 

    A HPLC method is established for the simultaneous determination of four phenolic acids (gallic acid, vanillic acid, caffeic acid and p-coumaric acid) in the extract of Terminalia. XDB-C18 (4.6 x 150 mm i.d., 5 µm) column was used, the chromatography was carried out with a linear gradient...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics