TITLE

Phylogenetic analysis of two Chinese orf virus isolates based on sequences of B2L and VIR genes

AUTHOR(S)
Li, Huixia; Zhu, Xueliang; Zheng, Yadong; Wang, Shuai; Liu, Zhenyong; Dou, Yongxi; Li, Hui; Cai, Xuepeng; Luo, Xuenong
PUB. DATE
July 2013
SOURCE
Archives of Virology;Jul2013, Vol. 158 Issue 7, p1477
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Orf virus (ORFV) is an enveloped virus with a double-stranded DNA genome, causing a contagious pustular dermatitis, mainly in goats and sheep. In this study, two strains of ORFV were isolated from sheep and goat samples in Xinjiang and Shaanxi, China. The B2L and virus interferon resistance (VIR) genes of these two isolates were sequenced and analyzed after PCR amplification. Phylogenetic analysis showed that the two isolates clustered with other ORFV strains but were separated into different subgroups. The Xinjiang strain shared the highest homology with the Gansu strain, whereas the Shaanxi strain shared higher homology with the Taiwan and Hubei strains. This is the first report of the molecular characterization of ORFV in Northwest China, and it provides new information on the genotyping of the causative agents responsible for contagious ecthyma dermatitis outbreaks in China.
ACCESSION #
88428255

 

Related Articles

  • Rapid detection of orf virus by loop-mediated isothermal amplification based on the DNA polymerase gene. Li, Jida; Song, Deguang; He, Wenqi; Bao, Yingfu; Lu, Rongguang; Su, Gaoli; Wang, Gaili; Lu, Huijun; Zhao, Kui; Gao, Feng // Archives of Virology;Apr2013, Vol. 158 Issue 4, p793 

    At present, there are no effective antiviral treatments available for contagious ecthyma, and rapid diagnosis is therefore critical for effective control of the disease. Recently, the invention of a novel LAMP technique that can rapidly amplify nucleic acids with high specificity and sensitivity...

  • Isolation and characterization of West Nile virus from the blood of viremic patients during the 2000 outbreak in Israel. Hindiyeh, Musa; Shulman, Lester M.; Mendelson, Ella; Weiss, Lea; Grossman, Zehava; Bin, Hanna; Hindiyeh, M; Shulman, L M; Mendelson, E; Weiss, L; Grossman, Z; Bin, H // Emerging Infectious Diseases;Jul/Aug2001, Vol. 7 Issue 4, p748 

    We report the isolation of West Nile (WN) virus from four patient serum samples submitted for diagnosis during an outbreak of WN fever in Israel in 2000. Sequencing and phylogenetic analysis revealed two lineages, one closely related to a 1999 New York isolate and the other to a 1999 Russian...

  • Orf Virus Infection in a Hunter in Western Austria, Presumably Transmitted by Game. KITCHEN, Maria; MÜLLER, Hansgeorg; ZOBL, Alexandra; WINDISCH, Andrea; ROMANI, Nikolaus; HUEMER, Hartwig // Acta Dermato-Venereologica;2/26/2014, Vol. 94 Issue 2, p212 

    A variety of animals host parapoxviruses. Orf virus is prevalent in sheep and goats in the Tyrol region of Austria and Northern Italy. Zoonotic infections in humans mostly occur after occupational exposure. We report here a case of a hunter with a typical Orf lesion (contagious ecthyma) on the...

  • PRESENCE OF BOVINE HERPESVIRUS TYPE 4 (BHV-4) INFECTION IN BULLS FOR ARTIFICIAL INSEMINATION IN SERBIA. Nikolin, V.; Vesna, Milićević; Radosavljević, V. // Acta Veterinaria;2008, Vol. 58 Issue 2/3, p267 

    Bovine herpesvirus type 4 (BHV-4) is a worldwide distributed gammaherpesvirus, with different antigenic and biologic properties from all the other bovine herpesviruses. In vivo distribution of BHV-4 was examined by testing various organs of experimentally infected calves. However, limited data...

  • Easy and efficient protocol for oomycete DNA extraction suitable for population genetic analysis. Zelaya-Molina, Lily X.; Ortega, Maria A.; Dorrance, Anne E. // Biotechnology Letters;Apr2011, Vol. 33 Issue 4, p715 

    Purpose of work: A simple and rapid DNA extraction protocol capable of obtaining high-quality and quantity DNA from a large number of individuals is essential for assaying population and phylogenetic studies of plant pathogens. Most DNA extraction protocols used with oomycetes are relatively...

  • Molecular epidemiology of TT virus (TTV) and characterization of two novel TTV genotypes in Indonesia. Muljono, D. H.; Nishizawa, T.; Tsuda, F.; Takahashi, M.; Okamoto, H. // Archives of Virology;Jul2001, Vol. 146 Issue 7, p1249 

    Summary. The prevalence of TT virus (TTV) DNA among 244 healthy individuals in 23 cities on 12 islands in Indonesia was determined by polymerase chain reaction (PCR) with primers derived from the coding region (N22), which can detect TTV DNA of genotypes 1–6. By N22 PCR, TTV DNA was...

  • Development of a novel TaqMan-based real-time PCR assay for the detection of porcine boca-like virus (Pbo-likeV).  // Virology Journal;2011, Vol. 8 Issue 1, p357 

    The article presents a study that developed a real-time assay for porcine boca-like virus (Pbo-likeV), a member of the Parvoviridae family, genus Bocavirus. A sensitive and specific TaqMan-based real-time PCR assay to target the Pbo-likeV NP1 gene was developed during the study. It is mentioned...

  • A novel chromosomal translocation t(3;7)(q26;q21) in myeloid leukemia resulting in overexpression of EVI1. Storlazzi, C. T.; Anelli, L.; Albano, F.; Zagaria, A.; Ventura, M.; Rocchi, M.; Panagopoulos, I.; Pannunzio, A.; Ottaviani, E.; Liso, V.; Specchia, G. // Annals of Hematology;Feb2004, Vol. 83 Issue 2, p78 

    The EVI1 proto-oncogene encodes a nuclear zinc finger protein that acts as a transcription repressor factor. In myeloid leukemia it is often activated by chromosomal rearrangements involving band 3q26, where the gene has been mapped. Here we report two leukemia cases [a chronic myeloid leukemia...

  • Nhumirim virus, a novel flavivirus isolated from mosquitoes from the Pantanal, Brazil. Pauvolid-Corrêa, Alex; Solberg, Owen; Couto-Lima, Dinair; Kenney, Joan; Serra-Freire, Nicolau; Brault, Aaron; Nogueira, Rita; Langevin, Stanley; Komar, Nicholas // Archives of Virology;Jan2015, Vol. 160 Issue 1, p21 

    We describe the isolation of a novel flavivirus, isolated from a pool of mosquitoes identified as Culex ( Culex) chidesteri collected in 2010 in the Pantanal region of west-central Brazil. The virus is herein designated Nhumirim virus (NHUV) after the name of the ranch from which the mosquito...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics