High-Resolution, High-Throughput, Positive-Tone Patterning of Poly(ethylene glycol) by Helium Beam Exposure through Stencil Masks

Cacao, Eliedonna E.; Nasrullah, Azeem; Sherlock, Tim; Kemper, Steven; Kourentzi, Katerina; Ruchhoeft, Paul; Stein, Gila E.; Willson, Richard C.
May 2013
PLoS ONE;May2013, Vol. 8 Issue 5, p1
Academic Journal
In this work, a collimated helium beam was used to activate a thiol-poly(ethylene glycol) (SH-PEG) monolayer on gold to selectively capture proteins in the exposed regions. Protein patterns were formed at high throughput by exposing a stencil mask placed in proximity to the PEG-coated surface to a broad beam of helium particles, followed by incubation in a protein solution. Attenuated Total Reflectance–Fourier Transform Infrared Spectroscopy (ATR–FTIR) spectra showed that SH-PEG molecules remain attached to gold after exposure to beam doses of 1.5–60 µC/cm2 and incubation in PBS buffer for one hour, as evidenced by the presence of characteristic ether and methoxy peaks at 1120 cm−1 and 2870 cm−1, respectively. X-ray Photoelectron Spectroscopy (XPS) spectra showed that increasing beam doses destroy ether (C–O) bonds in PEG molecules as evidenced by the decrease in carbon C1s peak at 286.6 eV and increased alkyl (C–C) signal at 284.6 eV. XPS spectra also demonstrated protein capture on beam-exposed PEG regions through the appearance of a nitrogen N1s peak at 400 eV and carbon C1s peak at 288 eV binding energies, while the unexposed PEG areas remained protein-free. The characteristic activities of avidin and horseradish peroxidase were preserved after attachment on beam-exposed regions. Protein patterns created using a 35 µm mesh mask were visualized by localized formation of insoluble diformazan precipitates by alkaline phosphatase conversion of its substrate bromochloroindoyl phosphate-nitroblue tetrazolium (BCIP-NBT) and by avidin binding of biotinylated antibodies conjugated on 100 nm gold nanoparticles (AuNP). Patterns created using a mask with smaller 300 nm openings were detected by specific binding of 40 nm AuNP probes and by localized HRP-mediated deposition of silver nanoparticles. Corresponding BSA-passivated negative controls showed very few bound AuNP probes and little to no enzymatic formation of diformazan precipitates or silver nanoparticles.


Related Articles

  • An anchoring strategy for photoswitchable biosensor technology: azobenzene-modified SAMs on Si(111). Dietrich, P.; Michalik, F.; Schmidt, R.; Gahl, C.; Mao, G.; Breusing, M.; Raschke, M.; Priewisch, B.; Elsässer, T.; Mendelsohn, R.; Weinelt, M.; Rück-Braun, K. // Applied Physics A: Materials Science & Processing;Nov2008, Vol. 93 Issue 2, p285 

    A mild and efficient procedure has been developed to obtain covalently attached self-assembled monolayers (SAMs) on Si(111) with photochromic azobenzene head-groups. Starting from neat or diluted carboxylic acid functionalized monolayers on-chip coupling reactions were applied to attach...

  • The effect of PEG encapsulated silver nanoparticles on the thermal and electrical property of sonochemically synthesized polyaniline/silver nanocomposite. Mishra, Satyendra; Shimpi, Navinchandra; Sen, Tanushree // Journal of Polymer Research;Jan2013, Vol. 20 Issue 1, p1 

    An investigation of microstructure of polyaniline nanocomposite is presented. The highly stable, semiconducting polyaniline/silver nanocomposites are prepared in-situ, employing the acoustic cavitation mechanism to obtain the composite in nanophase. The materials are characterized by UV-visible...

  • Shear-thickening behavior of modified silica nanoparticles in polyethylene glycol. Yu, Kejing; Cao, Haijian; Qian, Kun; Sha, Xiaofei; Chen, Yanping // Journal of Nanoparticle Research;Mar2012, Vol. 14 Issue 3, p1 

    The surface of silica nanoparticles with an average diameter of 650 nm was modified by ball milling and chemical treatment with ethylene glycol (EG). The Fourier-transform infrared spectra and particle size analysis methods were used to characterize the modified silica nanoparticles. The...

  • Formulation and Evaluation of Immediate Release Tablets of Eprosartan Mesylate. Satyavathi, K.; Annapurna, K. V. S. S.; Gayathri, P.; Bhojaraju, P.; Kanthal, L. K.; Veerraju, T. // International Journal of Pharmaceutical Sciences Review & Resear;Mar/Apr2014, Vol. 25 Issue 1, p36 

    The aim of present work is to formulate and evaluate the immediate release tablets of Eprosartan mesylate by using solid dispersion (PEG) and kneading method. Polyethylene glycol 6000 was used as a carrier in solid dispersion (fusion method). The drug and carrier were taken in different ratios...

  • Solvothermal Synthesis of Gd2O3 :Eu3+ Luminescent Nanowires. Guixia Liu; Song Zhang; Xiangting Dong; Jinxian Wang // Journal of Nanomaterials;2010, Special section p1 

    Uniform Gd2O3 : Eu3+ luminescent nanowires were prepared on a large scale by a facile solvothermal method using polyethylene glycol (PEG-2000) as template and ethanol as solvent; the properties and the structure were characterized. X-ray diffraction (XRD) patterns and Fourier transform infrared...

  • Effects of adding nano metal powders on thermooxidative degradation of poly(ethylene glycol). Lin, Z.; Han, X.; Wang, T.; Li, S. // Journal of Thermal Analysis & Calorimetry;Mar2008, Vol. 91 Issue 3, p709 

    Effects of nano-metal powders (aluminum and nickel) addition on the thermooxidative degradation of binder PEG in air atmosphere from 20 to 350°C were studied by TG/DTA and in-situ FTIR. TG/DTA results showed that the addition of nano-Al slowed down the degradation process of PEG in the early...

  • In-vitro dissolution enhancement of aceclofenac using hydrophilic carriers. Samal, Himansu Bhusan; Naveen Kumar, N.; Sneha, S. // Journal of Pharmacy Research;2012, Vol. 5 Issue 8, p4496 

    The aim of the present study was to enhance the solubility and dissolution rate of poorly water-soluble drug Aceclofenac (BCS-class II) using its solid dispersions (SDs) with hydrophilic carrier's polyethylene glycol (PEG) 6000 and Inclusion complex with β-Cyclodextrin. SDs of Aceclofenac...

  • Synthesis and Characterizations of Poly(trimethylene terephthalate)-b-poly(tetramethylene glycol) Copolymers. Feng Liu; Meng Yao; Ming-tao Run // International Journal of Polymer Science;2013, p1 

    A series of poly (trimethylene terephthalate)-b-poly (tetramethylene glycol) (PTT-PTMEG) copolymers were synthesized by twostep melt-polycondensation. The copolymers were characterized by using Fourier transform infrared spectroscopy (FTIR), 1H NMR spectroscopy, rheometer, differential scanning...

  • Rapid Biosynthesis of Silver nanoparticles using Fenugreek leaves. Suganya, T. R.; Devasena, T. // International Journal of ChemTech Research;May/Jun2014, Vol. 6 Issue 3, p2156 

    Spherical shaped silver nanoparticles were synthesized using Fenugreek leaves extract and the prepared nanoparticles were characterized for their structural, optical and morphological properties. The Fourier transformer infrared spectroscopy reveals the presence of polyphenolic compounds in...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics