Differentiating ES cells
- Functional gene screening in embryonic stem cells implicates Wnt antagonism in neural differentiation. Aubert, Jerôme; Dunstan, Hannah; Chambers, Ian; Smith, Austin // Nature Biotechnology;Dec2002, Vol. 20 Issue 12, p1240
The multilineage differentiation capacity of mouse embryonic stem (ES) cells offers a potential testing platform for gene products that mediate mammalian lineage determination and cellular specialization. Identification of such differentiation regulators is crucial to harnessing ES cells for...
- Forced expression of Genesis, a winged helix transcriptional repressor isolated from embryonic stem cells, blocks granulocytic differentiation of 32D myeloid cells. Xu, D; Yoder, M; Sutton, J; Hromas, R // Leukemia (08876924);Feb98, Vol. 12 Issue 2, p207
We recently isolated and characterized a novel member of the winged helix (formerly HNF-3/Forkhead) transcriptional regulatory family, termed Genesis. Genesis was found to be a transcriptional repressor expressed almost exclusively in embryonic stem cells or embryonal carcinoma cells. This...
- BMP4 initiates human embryonic stem cell differentiation to trophoblast. Xu, Ren-He; Chen, Xin; Li, Dong S.; Li, Rui; Addicks, Gregory C.; Glennon, Clay; Zwaka, Thomas P.; Thomson, James A. // Nature Biotechnology;Dec2002, Vol. 20 Issue 12, p1261
The excitement and controversy surrounding the potential role of human embryonic stem (ES) cells in transplantation therapy have often overshadowed their potentially more important use as a basic research tool for understanding the development and function of human tissues. Human ES cells can...
- Signposts on the neural path. Gottlieb, David // Nature Biotechnology;Dec2002, Vol. 20 Issue 12, p1208
Reports on studies that elucidate the genetic pathways that control the in vitro differentiation of embryonic stem (ES) cells into neural cells. Genetic methods used by Aubert et al in their study; Similarity of basic features of control in two systems demonstrated by the study of Wichterle et al.
- Stem Cells Grow Challenges. Tabar, Viviane // Genomics & Proteomics;Nov/Dec2003, Vol. 3 Issue 9, p11
Discusses the challenges presented by humam embryonic stem cells. Efforts on directing embryonic stem cell differentiation into specific fates; Development of protocols for differentiation into other targets; Importance of exploration in the biology of stem cells in the knowledge of development...
- Feeder-free growth of undifferentiated human embryonic stem cells. Xu, Chunhui; Inokuma, Margaret S.; Denham, Jerrod; Golds, Kathaleen; Kundu, Pratima; Gold, Joseph D.; Carpenter, Melissa K. // Nature Biotechnology;Oct2001, Vol. 19 Issue 10, p971
Previous studies have shown that maintenance of undifferentiated human embryonic stem (hES) cells requires culture on mouse embryonic fibroblast (MEF) feeders. Here we demonstrate a successful feeder-free hES culture system in which undifferentiated cells can be maintained for at least 130...
- Gut Enriched Kruppel-Like Factor (GKLF) Inhibits Differentiation and Induces Self-Renewal of Murine Embryonic Stem Cells // Molecular Therapy;May2004 Supplement 1, Vol. 9, p22
An abstract of the article "Gut Enriched Kruppel-Like Factor (GKLF) Inhibits Differentiation and Induces Self-Renewal of Murine Embryonic Stem Cells," by Rebecca J. Chan, Yanjun Li, Li Zhong, and Mervin C. Yoder is presented.
- Hematopoietic cell differentiation of common marmoset (Callithrix jacchus) embryonic stem cells and their genetic manipulation using the third generation lentiviral vector // Molecular Therapy;May2004 Supplement 1, Vol. 9, p347
An abstract of the article "Hematopoietic Cell Differentiation of Common Marmoset (Callithrix jacchus) Embryonic Stem Cells and Their Genetic Manipulation Using the Third Generation Lentiviral Vector," by Ryo Kurita, Erika Sasaki and Takashi Hiroyama is presented.
- Stem cells with brainpower. Studer, Lorenz // Nature Biotechnology;Dec2001, Vol. 19 Issue 12, p1117
Reports on two studies that provide insight into how human embryonic stem (ES) cell potential can be harnessed toward the generation of brain cells. Diseases that may be treatable with human ES cell research; Induction of neural differentiation by overgrowth of undifferentiated ES cells;...