TITLE

HPLC METHOD DEVELOPMENT OF AMLODIPINE BY RP-HPLC IN ITS BULK DOSAGE FORMS

AUTHOR(S)
Kumar, V. Naveen; kumar, K. Kiran; Babu, P. Ramesh; Anilkumar, K.
PUB. DATE
June 2012
SOURCE
International Journal of Pharmaceutical Sciences & Research;Jun2012, Vol. 3 Issue 6, p1675
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A new, simple, precise, sensitive, accurate and reproducible Reverse phase HPLC method was developed and validated for the analysis of amlodipine in bulk dosage forms. The separation was conducted by using c-18 RP-HPLC coloumn, which was maintained at ambient temperature. The mobile phase consisting of Phosphate buffer and Acetonitrile (90:10v/v) was delivered at a rate of 1.5 ml/min. The analysis was detected by using UV detector at the wave length of 225nm.The method is validated for its specificity, precision, accuracy, linearity and robustness. The method was found to be linear over the concentration range 10-100 µg/ml (r ² =0.999). The retention time for amlodipine was found to be 3.34min. Limit of quantification of the method is 0.179 µg/ml and limit of detection is 0.054 µg/ml.
ACCESSION #
88282641

 

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