TITLE

Gene expression analysis by transcript profiling coupled to a gene database query

AUTHOR(S)
Shimkets, Richard A.; Lowe, David G.; Tai, Julie Tsu-Ning; Sehl, Patricia; Jin, Hongkui; Yang, Renhui; Predki, Paul F.; Rothberg, Bonnie E. G.; Murtha, Michael T.; Roth, Matthew E.; Shenoy, Suresh G.; Windemuth, Andreas; Simpson, John W.; Simons, Jan F.; Daley, Michael P.; Gold, Steven A.; McKenna, Michael P.; Hillan, Kenneth; Went, Gregory T.
PUB. DATE
August 1999
SOURCE
Nature Biotechnology;Aug99, Vol. 17 Issue 8, p798
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
We describe an mRNA profiling technique for determining differential gene expression that utilizes, but does not require, prior knowledge of gene sequences. This method permits high-throughput reproducible detection of most expressed sequences with a sensitivity of greater than 1 part in 100,000. Gene identification by database query of a restriction endonuclease fingerprint, confirmed by competitive PCR using gene-specific oligonucleotides, facilitates gene discovery by minimizing isolation procedures. This process, called GeneCalling, was validated by analysis of the gene expression profiles of normal and hypertrophic rat hearts following in vivo pressure overload.
ACCESSION #
8818589

 

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