TITLE

Current state of automated crystallographic data analysis

AUTHOR(S)
Lamzin, Victor S.; Perrakis, Anastassis
PUB. DATE
November 2000
SOURCE
Nature Structural Biology;Nov2000 Supplement, Vol. 7, p978
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
A goal of structural biology ? and of structural genomics in particular ? is to improve the underlying methodology for high-throughput determination of three-dimensional structures of biological macromolecules. Here we address issues related to the development, automation and streamlining of the process of macromolecular X-ray crystal structure solution.
ACCESSION #
8817371

 

Related Articles

  • Protein NMR spectroscopy in structural genomics. Montelione, Gaetano T.; Zheng, Deyou; Huang, Yuanpeng J.; Gunsalus, Kristin C.; Szyperski, Thomas // Nature Structural Biology;Nov2000 Supplement, Vol. 7, p982 

    Protein NMR spectroscopy provides an important complement to X-ray crystallography for structural genomics, both for determining three-dimensional protein structures and in characterizing their biochemical and biophysical functions.

  • Structural genomics projects in Japan. Yokoyama, Shigeyuki; Hirota, Hiroshi; Kigawa, Takanori; Yabuki, Takashi; Shirouzu, Mikako; Terada, Takaho; Ito, Yutaka; Matsuo, Yo; Kuroda, Yutaka; Nishimura, Yoshifumi; Kyogoku, Yoshimasa; Miki, Kunio; Masui, Ryoji; Kuramitsu, Seiki // Nature Structural Biology;Nov2000 Supplement, Vol. 7, p943 

    Two major structural genomics projects exist in Japan. The oldest, the RIKEN Structural Genomics Initiative, has two major goals: to determine bacterial, mammalian, and plant protein structures by X-ray crystallography and NMR spectroscopy and to perform functional analyses with the target...

  • Prediction of Protein Disorder at the Domain Level. Dosztanyi, Zsuzsanna; Sandor, Mark; Tompa, Peter; Simon, Istvan // Current Protein & Peptide Science;Apr2007, Vol. 8 Issue 2, p161 

    Intrinsically disordered/unstructured proteins exist in a highly flexible conformational state largely devoid of secondary structural elements and tertiary contacts. Despite their lack of a well defined structure, these proteins often fulfill essential regulatory functions. The intrinsic lack of...

  • Protein Structure Prediction Using an Augmented Homology Modeling Method: Key Importance of Iterative-Procedures for Obtaining Consistent Quality Models. McDonald, Shawn; Mylvaganam, Shankari; Shenderovich, Mark; Tseitin, Vladimir; Fisher, Cindy; Raghunathan, Gopalan; Jinhua Zheng; Kodandapani, Ramadurgam; Dudek, Michael; Prabhakaran, Muthuchi-dambaram; Ramnarayan, Kal // Current Proteomics;Oct2005, Vol. 2 Issue 3, p233 

    Deciphering of the human genome and other model genomes presents the challenge of unraveling the gene products that are expressed by these genomes, and identifying the functional role of the expressed proteins. Rapid determination of the three-dimensional (3D) structures of the gene products is...

  • Making decisions for structural genomics. Rodrigues, Ana; Hubbard, Roderick E. // Briefings in Bioinformatics;Jun2003, Vol. 4 Issue 2, p150 

    A large number of structural genomics programmes have been established worldwide with the common aim of large-scale, high-throughput protein structure determination. Due to the considerable challenges posed by the experimental methods of structural determination (primarily X-ray crystallography...

  • CRYSpred: Accurate Sequence-Based Protein Crystallization Propensity Prediction Using Sequence-Derived Structural Characteristics. Mizianty, Marcin J.; Kurgan, Lukasz A. // Protein & Peptide Letters;Jan2012, Vol. 19 Issue 1, p40 

    Relatively low success rates of X-ray crystallography, which is the most popular method for solving proteins structures, motivate development of novel methods that support selection of tractable protein targets. This aspect is particularly important in the context of the current structural...

  • Residual dipolar couplings: Synergy between NMR and structural genomics. Al-Hashimi, Hashim M.; Patel, Dinshaw J. // Journal of Biomolecular NMR;Jan2002, Vol. 22 Issue 1, p1 

    Structural genomics is on a quest for the structure and function of a significant fraction of gene products. Current efforts are focusing on structure determination of single-domain proteins, which can readily be targeted by X-ray crystallography, NMR spectroscopy and computational homology...

  • G-protein-coupled receptor structure, ligand binding and activation as studied by solid-state NMR spectroscopy. Xiaoyan DING; Xin ZHAO; WATTS, Anthony // Biochemical Journal;3/15/2013, Vol. 450 Issue 3, p443 

    GPCRs (G-protein-coupled receptors) are versatile signalling molecules at the cell surface and make up the largest and most diverse family of membrane receptors in the human genome. They convert a large variety of extracellular stimuli into intracellular responses through the activation of...

  • Sequence-Based Protein Crystallization Propensity Prediction for Structural Genomics: Review and Comparative Analysis. Kurgan, L.; Mizianty, M. J. // Natural Science;Sep2009, Vol. 1 Issue 2, p93 

    Structural genomics (SG) is an international effort that aims at solving three-dimensional shapes of important biological macro-molecules with primary focus on proteins. One of the main bottlenecks in SG is the ability to produce dif-fraction quality crystals for X-ray crystallography based...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics