Iron biofortification of Myanmar rice

May Sann Aung; Masuda, Hiroshi; Kobayashi, Takanori; Nakanishi, Hiromi; Yamakawa, Takashi; Nishizawa, Naoko K.
May 2013
Frontiers in Plant Science;May2013, Vol. 4, p1
Academic Journal
Iron (Fe) deficiency elevates human mortality rates, especially in developing countries. In Myanmar, the prevalence of Fe-deficient anemia in children and pregnant women are 75 and 71%, respectively. Myanmar people have one of the highest per capita rice consumption rates globally. Consequently, production of Fe-biofortified rice would likely contribute to solving the Fe-deficiency problem in this human population. To produce Fe-biofortified Myanmar rice by transgenic methods, we first analyzed callus induction and regeneration efficiencies in 15 varieties that are presently popular because of their high-yields or high-qualities. Callus formation and regeneration efficiency in each variety was strongly influenced by types of culture media containing a range of 2,4-dichlorophenoxyacetic acid concentrations. The Paw San Yin variety, which has a high-Fe content in polished seeds, performed well in callus induction and regeneration trials. Thus, we transformed this variety using a gene expression cassette that enhanced Fe transport within rice plants through overexpression of the nicotianamine synthase gene HvNAS', Fe flow to the endosperm through the Fe(II)-nicotianamine transporter gene OsYSL2, and Fe accumulation in endosperm by the Fe storage protein gene SoyferH2. A line with a transgene insertion was successfully obtained. Enhanced expressions of the introduced genes OsYSL2, HvNAS', and SoyferH2 occurred in immature T2 seeds. The transformants accumulated 3.4-fold higher Fe concentrations, and also 1.3-fold higher zinc concentrations in T2 polished seeds compared to levels in non-transgenic rice.This Fe-biofortified rice has the potential to reduce Fe-deficiency anemia in millions of Myanmar people without changing food habits and without introducing additional costs.


Related Articles

  • Nicotianamine synthase overexpression positively modulates iron homeostasis-related genes in high iron rice. Meng Wang; Gruissem, Wilhelm; Bhullar, Navreet K. // Frontiers in Plant Science;May2013, Vol. 4, p1 

    Nearly one-third of the world population, mostly women and children, suffer from iron malnutrition and its consequences, such as anemia or impaired mental development. Biofortification of rice, which is a staple crop for nearly half of the world's population, can significantly contribute in...

  • Expression analysis of two rice pollen-specific promoters using homologous and heterologous systems. Nguyen, Tien; Oo, Moe; Moon, Sunok; Bae, Hyun-Kyung; Oh, Sung; Soh, Moon-Soo; Song, Jong; Kim, Jeong; Jung, Ki; Park, Soon // Plant Biotechnology Reports;Sep2015, Vol. 9 Issue 5, p297 

    Tissue-specific promoters are a very useful tool for manipulating gene expression in a target tissue or organ; however, their range of applications in other plant species has not been determined, to date. In this study, we identified two late pollen-specific rice promoters ( ProOsLPS10 and...

  • Relationship between Disease Resistance and Rice Oxalate Oxidases in Transgenic Rice. Zhang, Xian Yong; Nie, Zhuan Hua; Wang, Wen Juan; Leung, David W. M.; Xu, Da Gao; Chen, Bai Ling; Chen, Zhe; Zeng, Lie Xian; Liu, E. E. // PLoS ONE;Oct2013, Vol. 8 Issue 10, p1 

    Differential expression of rice oxalate oxidase genes (OsOxO1-4) in rice leaves (Oryza sativa L.) in response to biotic stress was assayed using RT-PCR. OsOxO4 was induced transiently at 12 h in plants inoculated with the pathogens of bacterial blight and that of the wounding control....

  • Serotonin accumulation in transgenic rice by over-expressing tryptophan decarboxlyase results in a dark brown phenotype and stunted growth. Kanjanaphachoat, Parawee; Wei, Bi-Yin; Lo, Shuen-Fang; Wang, I-Wen; Wang, Chang-Sheng; Yu, Su-May; Yen, Ming-Liang; Chiu, Sheng-Hsien; Lai, Chien-Chen; Chen, Liang-Jwu // Plant Molecular Biology;Apr2012, Vol. 78 Issue 6, p525 

    A mutant M47286 with a stunted growth, low fertility and dark-brown phenotype was identified from a T-DNA-tagged rice mutant library. This mutant contained a copy of the T-DNA tag inserted at the location where the expression of two putative tryptophan decarboxlyase genes, TDC- 1 and TDC- 3,...

  • Translational downregulation of RBCL is operative in the coordinated expression of Rubisco genes in senescent leaves in rice. Suzuki, Yuji; Makino, Amane // Journal of Experimental Botany;Apr2013, Vol. 64 Issue 4, p1145 

    Rubisco gene expression was examined in detail in rice (Oryza sativa L.) leaves at different positions, i.e. expanding, mature, and senescent leaves. Rubisco small subunit (RBCS) synthesis and RBCS mRNA levels were maximal in expanding leaves and gradually became lower in mature and senescent...

  • Genetic and Molecular Analysis of Transgenic Rice cv. Rojolele Expressing Lactoferrin. Rachmawati, Diah; Daryono, Budi Setiadi; Nuringtyas, Tri Rini; Hiroyuki Anzai // Journal of Agricultural Science (1916-9752);Mar2014, Vol. 6 Issue 3, p1 

    In a previous study, human lactoferrin gene have introduced into Javanica rice cv. Rojolele by Agrobacterium-mediated transformation. Lactoferrin (LF) is an 80 kDa iron-binding glycoprotein that has been proposed to have many biological roles such as protection against microbial and virus...

  • Enhanced resistance to stripe rust disease in transgenic wheat expressing the rice chitinase gene RC24. Huang, Xuan; Wang, Jian; Du, Zhen; Zhang, Chen; Li, Lan; Xu, Ziqin // Transgenic Research;Oct2013, Vol. 22 Issue 5, p939 

    Stripe rust is a devastating fungal disease of wheat worldwide which is primarily caused by Puccinia striiformis f. sp tritici. Transgenic wheat ( Triticum aestivum L.) expressing rice class chitinase gene RC24 were developed by particle bombardment of immature embryos and tested for resistance...

  • Identification of Transcription Factors Involved in Rice Secondary Cell Wall Formation. Hirano, Ko; Kondo, Mari; Aya, Koichiro; Miyao, Akio; Sato, Yutaka; Antonio, Baltazar A.; Namiki, Nobukazu; Nagamura, Yoshiaki; Matsuoka, Makoto // Plant & Cell Physiology;Nov2013, Vol. 54 Issue 11, p1791 

    Using co-expression network analysis, we identified 123 transcription factors (TFs) as candidate secondary cell wall regulators in rice. To validate whether these TFs are associated with secondary cell wall formation, six TF genes belonging to the MYB, NAC or homeodomain-containing TF families...

  • OsBAT1 Augments Salinity Stress Tolerance by Enhancing Detoxification of ROS and Expression of Stress-Responsive Genes in Transgenic Rice. Tuteja, Narendra; Sahoo, Ranjan; Huda, Kazi; Tula, Suresh; Tuteja, Renu // Plant Molecular Biology Reporter;Oct2015, Vol. 33 Issue 5, p1192 

    Coping with salinity-induced reduction in crop yield is essential for food security. Human leukocyte antigen-B associated transcript 1 (BAT1) also called as UAP56 is a DExD/H-box protein involved in messenger RNA (mRNA) splicing. Function of plant homologue of BAT1, especially its involvement in...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics