Nicotianamine synthase overexpression positively modulates iron homeostasis-related genes in high iron rice

Meng Wang; Gruissem, Wilhelm; Bhullar, Navreet K.
May 2013
Frontiers in Plant Science;May2013, Vol. 4, p1
Academic Journal
Nearly one-third of the world population, mostly women and children, suffer from iron malnutrition and its consequences, such as anemia or impaired mental development. Biofortification of rice, which is a staple crop for nearly half of the world's population, can significantly contribute in alleviating iron deficiency. NFP rice (transgenic rice expressing nicotianamine synthase, ferritin and phytase genes) has a more than six-fold increase in iron content in polished rice grains, resulting from the synergistic action of nicotianamine synthase (NAS) and ferritin transgenes. We investigated iron homeostasis in NFP plants by analyzing the expression of 28 endogenous rice genes known to be involved in the homeostasis of iron and other metals, in iron-deficient and iron-sufficient conditions. RNA was collected from different tissues (roots, flag leaves, grains) and at three developmental stages during grain filling. NFP plants showed increased sensitivity to iron-deficiency conditions and changes in the expression of endogenous genes involved in nicotianamine (NA) metabolism, in comparison to their non-transgenic siblings (NTS). Elevated transcript levels were detected in NFP plants for several iron transporters. In contrast, expression of OsYSL2, which encodes a member of yellow stripe like protein family, and a transporter of the NA-Fe(II) complex was reduced in NFP plants under low iron conditions, indicating that expression of OsYSL2 is regulated by the endogenous iron status. Expression of the transgenes did not significantly affect overall iron homeostasis in NFP plants, which establishes the engineered push-pull mechanism as a suitable strategy to increase rice endosperm iron content.


Related Articles

  • Iron biofortification of Myanmar rice. May Sann Aung; Masuda, Hiroshi; Kobayashi, Takanori; Nakanishi, Hiromi; Yamakawa, Takashi; Nishizawa, Naoko K. // Frontiers in Plant Science;May2013, Vol. 4, p1 

    Iron (Fe) deficiency elevates human mortality rates, especially in developing countries. In Myanmar, the prevalence of Fe-deficient anemia in children and pregnant women are 75 and 71%, respectively. Myanmar people have one of the highest per capita rice consumption rates globally. Consequently,...

  • Induction of ferritin synthesis by water deficit and iron excess in common bean ( Phaseolus vulgaris L.). DeLaat, Daiane; Colombo, Carlos; Chiorato, Alisson; Carbonell, Sergio // Molecular Biology Reports;Mar2014, Vol. 41 Issue 3, p1427 

    Ferritins are molecules for iron storage present in most living beings. In plants, ferritin is an essential iron homeostasis regulator and therefore plays a fundamental role in control of iron induced by oxidative stress or by excess of iron ions. Ferritin gene expression is modulated by various...

  • Corrigendum: Phylogenetic relationships and selective pressure on gene families related to iron homeostasis in land plants. Victoria, Filipe de Carvalho; Bervald, Clauber Mateus Priebe; da Maia, Luciano Carlos; Farias, Daniel da Rosa; de Sousa, Rogerio Oliveira; Panaud, Olivier; Costa de Oliveira, Antônio // Genome;Feb2013, Vol. 56 Issue 2, p129 

    A correction to the article "Phylogenetic Relationships and Selective Pressure on Gene Families Related to Iron Homeostasis in Land Plants" that was published in a 2012 issue of the journal is presented.

  • Expression analysis of two rice pollen-specific promoters using homologous and heterologous systems. Nguyen, Tien; Oo, Moe; Moon, Sunok; Bae, Hyun-Kyung; Oh, Sung; Soh, Moon-Soo; Song, Jong; Kim, Jeong; Jung, Ki; Park, Soon // Plant Biotechnology Reports;Sep2015, Vol. 9 Issue 5, p297 

    Tissue-specific promoters are a very useful tool for manipulating gene expression in a target tissue or organ; however, their range of applications in other plant species has not been determined, to date. In this study, we identified two late pollen-specific rice promoters ( ProOsLPS10 and...

  • Relationship between Disease Resistance and Rice Oxalate Oxidases in Transgenic Rice. Zhang, Xian Yong; Nie, Zhuan Hua; Wang, Wen Juan; Leung, David W. M.; Xu, Da Gao; Chen, Bai Ling; Chen, Zhe; Zeng, Lie Xian; Liu, E. E. // PLoS ONE;Oct2013, Vol. 8 Issue 10, p1 

    Differential expression of rice oxalate oxidase genes (OsOxO1-4) in rice leaves (Oryza sativa L.) in response to biotic stress was assayed using RT-PCR. OsOxO4 was induced transiently at 12 h in plants inoculated with the pathogens of bacterial blight and that of the wounding control....

  • A digital compendium of genes mediating the reversible phosphorylation of proteins in Fe-deficient Arabidopsis roots. Ping Lan; Wenfeng Li; Schmidt, Wolfgang // Frontiers in Plant Science;Jun2013, Vol. 4, p1 

    Post-translational modifications of proteins such as reversible phosphorylation provide an important but understudied regulatory network that controls important nodes in the adaptation of plants to environmental conditions. Iron (Fe) is an essential mineral nutrient for plants, but due to its...

  • Shoot to root communication is necessary to control the expression of iron-acquisition genes in Strategy I plants. García, María; Romera, Francisco; Stacey, Minviluz; Stacey, Gary; Villar, Eduardo; Alcántara, Esteban; Pérez-Vicente, Rafael // Planta;Jan2013, Vol. 237 Issue 1, p65 

    Previous research showed that auxin, ethylene, and nitric oxide (NO) can activate the expression of iron (Fe)-acquisition genes in the roots of Strategy I plants grown with low levels of Fe, but not in plants grown with high levels of Fe. However, it is still an open question as to how Fe acts...

  • Cadmium Toxicity Induced Alterations in the Root Proteome of Green Gram in Contrasting Response towards Iron Supplement. Muneer, Sowbiya; Hakeem, Khalid Rehman; Mohamed, Rozi; Jeong Hyun Lee // International Journal of Molecular Sciences;Apr2014, Vol. 15 Issue 4, p6343 

    Cadmium signifies a severe threat to crop productivity and green gram is a notably iron sensitive plant which shows considerable variation towards cadmium stress. A gel-based proteomics analysis was performed with the roots of green gram exposed to iron and cadmium combined treatments. The...

  • Mitochondrial ferritin is a functional iron-storage protein in cucumber (Cucumis sativus) roots. Vigani, Gianpiero; Tarantino, Delia; Murgia, Irene // Frontiers in Plant Science;Aug2013, Vol. 4, p1 

    In plants, intracellular Fe trafficking must satisfy chloroplasts' and mitochondrial demands for Fe without allowing its accumulation in the organelles in dangerous redox-active forms. Protein ferritin is involved in such homeostatic control, however its functional role in mitochondria,...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics