TITLE

Acclimation to different depths by the marine angiosperm Posidonia oceanica: transcriptomic and proteomic profiles

AUTHOR(S)
Dattolo, Emanuela; Gu, Jenny; Bayer, Philipp E.; Mazzuca, Silvia; Serra, Ilia A.; Spadafora, Antonia; Bernardo, Letizia; Natali, Lucia; Cavallini, Andrea; Procaccini, Gabriele
PUB. DATE
May 2013
SOURCE
Frontiers in Plant Science;May2013, Vol. 4, p1
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
For seagrasses, seasonal and daily variations in light and temperature represent the mains factors driving their distribution along the bathymetric cline. Changes in these environmental factors, due to climatic and anthropogenic effects, can compromise their survival. In a framework of conservation and restoration, it becomes crucial to improve our knowledge about the physiological plasticity of seagrass species along environmental gradients. Here, we aimed to identify differences in transcriptomic and proteomic profiles, involved in the acclimation along the depth gradient in the seagrass Posidonia oceanica, and to improve the available molecular resources in this species, which is an important requisite for the application of eco-genomic approaches. To do that, from plant growing in the shallow (-5m) and a deep (-25m) portions of a single meadow, (i) we generated two reciprocal EST (Expressed Sequences Tags) libraries using a Suppressive Subtractive Hybridization (SSH) approach, to obtain depth/specific transcriptional profiles, and (ii) we identified proteins differentially expressed, using the highly innovative USIS mass spectrometry methodology, coupled with 1D-SDS electrophoresis and labeling free approach. Mass spectra were searched in the open source Global Proteome Machine (GPM) engine against plant databases and with the X!Tandem algorithm against a local database. Transcriptional analysis showed both quantitative and qualitative differences between depths. EST libraries had only the 3% of transcripts in common. A total of 315 peptides belonging to 64 proteins were identified by mass spectrometry. ATP synthase subunits were among the most abundant proteins in both conditions. Both approaches identified genes and proteins in pathways related to energy metabolism, transport and genetic information processing, that appear o be the most involved in depth acclimation in P. oceanica. Their putative rules in acclimation to depth were discussed.
ACCESSION #
88067207

 

Related Articles

  • THERMOPREFERENCE AND THERMAL RESISTANCE OF JUVENILE STERLET ACIPENSER RUTHENUS L., GROWN UNDER DIFFERENT CONDITIONS. Golovanov, V. K.; Gerasimov, Yu. V.; Kapshay, D. S.; Vasyura, O. L. // Vestnik of Astrakhan State Technical University. Series: Fishing;3/ 1/2014, Issue 1, p62 

    The purpose of this study is to determine the final selected temperature (FST) during the winter period of two groups of young sterlet, previously grown in ponds and pools. During the initial period for determination of ST and stable area selection -- FST the method of «final...

  • RELAÇÃO ENTRE CONCENTRAÇÕES FOLIARES DE CARBOIDRATOS SOLÚVEIS TOTAIS E TOLERÂNCIA AO FRIO EM DIFERENTES ESPÉCIES DE Eucalyptus spp. Pitz Floriani, Mireli Moura; Steffens, Cristiano André; Chaves, Djalma Miler; Do Amarante, Cassandra Vidal Talamini; Pikart, Tiago Georg; Santos Ribeiro, Maitê dos // Ciência Florestal (01039954);mar2013, Vol. 23 Issue 1, p165 

    This work was carried out to evaluate the effects of the rustification on cold tolerance in Eucalyptus dunnii Maiden, Eucalyptus benthamii Maiden & Cambage, Eucalyptus grandis W. Hill ex Maiden, and Eucalyptus saligna Sm., by quantifying leaf contents of total soluble carbohydrates and proline....

  • EFFECTS OF WINTER SWIMMING AND WHOLE-BODY CRYOTHERAPY ON THE HEMATOLOGICAL AND RHEOLOGICAL PROPERTIES OF BLOOD IN REGULAR WINTER SWIMMERS AND INDIVIDUALS EXPOSED TO WHOLE-BODY CRYOTHERAPY. Teległów, Aneta; Dąbrowski, Zbigniew; Marchewka, Anna; Głodzik, Jacek; Rembiasz, Konrad; Krawczyk, Marcin; Marchewka, Jakub; Wcisło, Małgorzata; Ostrowski, Andrzej // Medicina Sportiva;2014, Vol. 18 Issue 2, p52 

    Introduction: Exposure to cold is one of the strongest physiological and psychological environmental stressors and induces an array of significant functional responses. Objective: To analyze the changes in morphological and rheological parameters of blood in regular winter swimmers and...

  • Response to Heat Stress in the United States: Are More Dying or Adapting?  // CO2 Science;12/30/2014, p2 

    The article discusses a study by J. F. Bobb and colleagues, published in the scientific journal "Environmental Health Perspectives" on the mortality and adaptation response of the U.S. population to heat stress. Topics include the concern raised by the United Nations Intergovernmental Panel on...

  • Winter mortality and cold stress in Yekaterinburg, Russia:... Donaldson, G.C.; Tchernjavskii, V.E.; Ermakov, S.P.; Bucher, K.; Keatinge, W.R. // BMJ: British Medical Journal (International Edition);02/14/98, Vol. 316 Issue 7130, p514 

    Evaluates patterns of winter mortality and strategies for protection against cold in Yekaterinburg, Russia. Methodology of the study; Findings on the relationship between mortality and temperature; Factors that contribute to increased deaths due to exposure to cold.

  • Stone Risk Rises With Temperature.  // Renal & Urology News;Nov2014, Vol. 13 Issue 11, p20 

    The article presents research indicating the association of high daily mean temperatures with increased kidney stone evidence.

  • NUCLEAR PROTEIN BINDING TO THE AP-1 SEQUENCE IN RAT ADRENAL MEDULLA AND HYPOTHALAMUS WITH AGE AND COLD. T�ner, N.; Larochelle, J. S.; Baker, H. V.; Scarpace, P. J. // Gerontologist;Oct1996 Supplement, Vol. 36 Issue 1, p17 

    The AP-1 regulatory element has been implicated in the cold-induced expression of tyrosine hydroxylase in the adrenal medulla (AM). Since this response is also impaired with age, we examined the cold-stimulated AP-1 transcription factor binding to an oligonucleotide with the consensus sequence...

  • AGE-RELATED ALTERATIONS IN THE RESPONSE OF BROWN ADIPOSE TISSUE TO CHRONIC COLD-EXPOSURE. Florez-Duquet, M.; Horwitz, B. A.; Mcdonald, R. B. // Gerontologist;Oct1996 Supplement, Vol. 36 Issue 1, p18 

    We have previously demonstrated that older male F344 rats exhibit a significant decrease in interscapular brown adipose tissue (IBAT) mass and thermogenic capacity, when compared to young adult rats. These changes are correlated with the decreased ability of older rats to maintain body...

  • Utilização de diferentes filmes plásticos como cobertura de abrigos para cultivo protegido. José Weselli de Sá Andrade; Max Farias Júnior; Marliana Araújo de Sousa; Anísio Corrêa Rocha // Acta Scientiarum: Agronomy;Jul-Sep2011, Vol. 33 Issue 3, p437 

    This project was developed in the Faculty of Engineering - Unesp, Campus of Ilha Solteira, in the municipality of Ilha Solteira - São Paulo State. We studied the environmental behavior under two conditions of protected environment, with plastic covering the soil and bare soil. It was...

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics