Multiplex PCR for Rapid Diagnosis of Gastrointestinal Tuberculosis

Sharma, Kusum; Sinha, Saroj Kant; Sharma, Aman; Nada, Ritambra; Prasad, Kaushal K.; Goyal, Kapil; Rana, Surinder Singh; Bhasin, Deepak Kumar; Sharma, Meera
April 2013
Journal of Global Infectious Diseases;Apr-Jun2013, Vol. 5 Issue 2, p49
Academic Journal
Background: Rapid and specific diagnosis of gastrointestinal tuberculosis (GITB) is of utmost importance. Aim: To evaluate Multiplex PCR (MPCR) using MPB64 and IS6110 primers specific for M. tuberculosis for rapid diagnosis of GITB. Materials and Methods: MPCR was performed on colonoscopy biopsy specimens on 11 GITB confirmed (culture/AFB/ histopathology was positive), 29 GITB suspected and 30 Non GITB (control group) patients. Results: MPB64 PCR had sensitivity and specificity of 90% and 100% for confirmed GITB cases. In 29 clinically diagnosed but unconfirmed GITB cases, MPCR was positive in 72.41%. MPCR was negative in all control group patients. The overall sensitivity and specificity of microscopy, culture, histopathology and MPCR was 5%, 2% 20% and 77.5% and 100%, 100%, 100% and 100% respectively. Conclusion: MPCR has good sensitivity and specificity in diagnosing gastrointestinal tuberculosis.


Related Articles

  • An early fortuitous finding. Surawicz, Christina M. // Cortlandt Forum;1/25/96, Vol. 9 Issue 1, p147 

    Looks at the diagnosis of a young man with upper gastrointestinal disease. How diagnosis was made; Analysis. INSET: Evaluation of occult blood and anemia in a young man..

  • Upper gastrointestinal endoscopy performed by nurses: scope for the future? Smale, S.; Bjarnason, I.; Forgacs, I.; Prasad, P.; Mukhood, M.; Wong, M.; Ng, A.; Mulcahy, H.E. // Gut;Aug2003, Vol. 52 Issue 8, p1090 

    Background: Previous researchers have shown that non-medical endoscopists can perform lower gastrointestinal endoscopy as safely and effectively as medical staff. However, it is not known if upper gastrointestinal endoscopy performed by medical and non-medical endoscopists in clinical practice...

  • Anesthesia-Assisted Sedation Getting Notice by Medicare. Hirsch, Ronald L. // Mayo Clinic Proceedings;Mar2014, Vol. 89 Issue 3, p425 

    A letter to the editor is presented in response to the article "Preprocedural Considerations in Gastrointestinal Endoscopy," by E. C. Gorospe and A. S. Oxentenko in the 2013 issue.

  • In reply--Anesthesia-Assisted Sedation Getting Notice by Medicare. Gorospe, Emmanuel C.; Oxentenko, Amy S. // Mayo Clinic Proceedings;Mar2014, Vol. 89 Issue 3, p425 

    A response by Emmanuel C. Gorospe and Amy S. Oxentenko to a letter to the editor about their article "Preprocedural Considerations in Gastrointestinal Endoscopy," in the 2013 issue is presented.

  • Confocal Laser Endomicroscopy: Applications in Clinical and Translational Science--A Comprehensive Review. Goetz, Martin // ISRN Pathology;2012, p1 

    Confocal laser endomicroscopy (CLE) is a novel tool in the endoscopist's armamentarium. It allows on-site histological information. The ability of gastroenterologists to interpret such microscopic information has been demonstrated in multiple studies fromthe upper and lower gastrointestinal...

  • ISOLATION AND CHARACTERIZATION OF HELICOBACTER PYLORI STRAINS FROM GASTRIC BIOPSIES OF ALGERIAN PATIENTS. Hassina, Guetarni; Ahmed, Bensoltane // OnLine Journal of Biological Sciences;2013, Vol. 13 Issue 2, p41 

    The objective of this study is to isolate and characterize strains Helicobacetr pylori from gastric biopsies by upper gastrointestinal endoscopy. After a rapid urease test, two Helicobacter pylori strains isolated from four patients on blood agar. After extraction of DNA from these strains from...

  • Gastrointestinal Polyposis and Nonpolyposis Syndromes. Coyle, Thomas // New England Journal of Medicine;6/1/95, Vol. 332 Issue 22, p1518 

    The article presents a letter to the editor in response to Dr. Rustgi's previously published research on hereditary polyposis and nonpolyposis syndromes.

  • Gastrointestinal Polyposis and Nonpolyposis Syndromes. Ellis, Mark F. // New England Journal of Medicine;6/1/95, Vol. 332 Issue 22, p1518 

    The article presents a letter to the editor in response to a previous article on predictive markers for risk of adenomatous polyposis.

  • Gastrointestinal Polyposis and Nonpolyposis Syndromes. Whitelaw, Stuart // New England Journal of Medicine;6/1/95, Vol. 332 Issue 22, p1518 

    The article presents a letter to the editor in response to a previous article on prediction of colonic polyposis.


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics