Selection of binding targets in parasites using phage-display and aptamer libraries in vivo and in vitro

Tonelli, R. R.; Colli, W.; Alves, M. J. M.
January 2013
Frontiers in Immunology;Jan2013, Vol. 4, p1
Academic Journal
Parasite infections are largely dependent on interactions between pathogen and different host cell populations to guarantee a successful infectious process. This is particularly true for obligatory intracellular parasites as Plasmodium, Toxoplasma, and Leishmania, to name a few. Adhesion to and entry into the cell are essential steps requiring specific parasite and host cell molecules. The large amount of possible involved molecules poses additional difficulties for their identification by the classical biochemical approaches. In this respect, the search for alternative techniques should be pursued. Among them two powerful methodologies can be employed, both relying upon the construction of highly diverse combinatorial libraries of peptides or oligonucleotides that randomly bind with high affinity to targets on the cell surface and are selectively displaced by putative ligands. These are, respectively, the peptide-based phage display and the oligonucleotide-based aptamer techniques. The phage display technique has been extensively employed for the identification of novel ligands in vitro and in vivo in different areas such as cancer, vaccine development, and epitope mapping. Particularly, phage display has been employed in the investigation of pathogen-host interactions. Although this methodology has been used for some parasites with encouraging results, in trypanosomatids its use is, as yet, scanty. RNA and DNA aptamers, developed by the SELEX process (Systematic Evolution of Ligands by Exponential Enrichment), were described over two decades ago and since then contributed to a large number of structured nucleic acids for diagnostic or therapeutic purposes or for the understanding of the cell biology. Similarly to the phage display technique scarce use of the SELEX process has been used in the probing of parasite-host interaction. In this review, an overall survey on the use of both phage display and aptamer technologies in different pathogenic organisms will be discussed. Using these techniques, recent results on the interaction of Trypanosoma cruzi with the host will be highlighted focusing on members of the 85 kDa protein family, a subset of the gp85/TS superfamily.


Related Articles

  • Seroepidemiology of Toxoplasma gondii, Neospora caninum, and Leishmania spp. infections and risk factors for cats from Brazil. Coelho, Willian; Amarante, Alessandro; Apolinário, Juliana de; Coelho, Natalia; Lima, Valéria; Perri, Silvia; Bresciani, Katia // Parasitology Research;Oct2011, Vol. 109 Issue 4, p1009 

    The seroprevalence of infection by Toxoplasma gondii, Neospora caninum, and Leishmania spp. was detected through an indirect immunofluorescence in 70 cats from the Andradina Municipality, São Paulo State, Brazil. Anti- T. gondii antibodies (titer >64) were detected in 15.7% (11/70) of...

  • Drug discovery: Priming the antimalarial pipeline. Fidock, David A. // Nature;5/20/2010, Vol. 465 Issue 7296, p297 

    The article presents several studies regarding the development of antimalarial drugs. It states that W. A. Guiguemde and colleagues screened compounds which inhibit the growth of Plasmodium falciparum in cultured red blood cells. It also says that the effect of compounds on other pathogenic...

  • Prevalence of antibodies to Leishmania infantum and Toxoplasma gondii in horses from the north of Portugal. Lopes, Ana Patrícia; Sousa, Susana; Dubey, J. P.; Ribeiro, Ana J.; Silvestre, Ricardo; Cotovio, Mário; Schallig, Henk D. F. H.; Cardoso, Luís; Cordeiro-da-Silva, Anabela // Parasites & Vectors;2013, Vol. 6 Issue 1, p1 

    Background: Leishmania infantum and Toxoplasma gondii are protozoa with zoonotic and economic importance. Prevalences of antibodies to these agents were assessed in 173 horses from the north of Portugal. Findings: Antibodies to L. infantum were detected by the direct agglutination test (DAT);...

  • Prevalence and Predictors of Toxoplasma Seropositivity in Women with and at Risk for Human Immunodeficiency Virus Infection. Falusi, Oluwatoyin; French, Audrey L.; Seaberg, Eric C.; Tien, Phyllis C.; Watts, D. Heather; Minkoff, Howard; Piessens, Eva; Kovacs, Andrea; Anastos, Kathryn; Cohen, Mardge H. // Clinical Infectious Diseases;12/1/2002, Vol. 35 Issue 11, p1414 

    We assessed the prevalence and predictors of latent Toxo-plasma infection in a large group of human immunodefi-ciency virus (HIV)--infected and HIV-uninfected at-risk US women. The prevalence of latent Toxoplasma infection was 15% (380 of 2525 persons) and did not differ by HIV infec-tion ...

  • Occurrence of antibodies anti- Neospora caninum, anti- Toxoplasma gondii, and anti- Leishmania chagasi in serum of dogs from Pará State, Amazon, Brazil. Valadas, Samantha; Minervino, Antonio; Lima, Valéria; Soares, Rodrigo; Ortolani, Enrico; Gennari, Solange // Parasitology Research;Jul2010, Vol. 107 Issue 2, p453 

    A cross-sectional study was conducted to determine the occurrence of anti- Toxoplasma gondii, anti- Neospora caninum, and anti- Leishmania chagasi antibodies in dogs of the state of Pará, Brazil. For this purpose, 129 blood samples were collected from dogs of different ages and gender....

  • A Toxoplasma gondii Pseudokinase Inhibits Host IRG Resistance Proteins. Fleckenstein, Martin C.; Reese, Michael L.; Könen-Waisman, Stephanie; Boothroyd, John C.; Howard, Jonathan C.; Steinfeldt, Tobias // PLoS Biology;Jul2012, Vol. 10 Issue 7, p1 

    The ability of mice to resist infection with the protozoan parasite, Toxoplasma gondii, depends in large part on the function of members of a complex family of atypical large GTPases, the interferon-gamma-inducible immunity-related GTPases (IRG proteins). Nevertheless, some strains of T. gondii...

  • TgCDPK3 Regulates Calcium-Dependent Egress of Toxoplasma gondii from Host Cells. McCoy, James M.; Whitehead, Lachlan; van Dooren, Giel G.; Tonkin, Christopher J. // PLoS Pathogens;Dec2012, Vol. 8 Issue 12, p1 

    The phylum Apicomplexa comprises a group of obligate intracellular parasites of broad medical and agricultural significance, including Toxoplasma gondii and the malaria-causing Plasmodium spp. Key to their parasitic lifestyle is the need to egress from an infected cell, actively move through...

  • Evolution of apoptosis-like programmed cell death in unicellular protozoan parasites. Kaczanowski, Szymon; Sajid, Mohammed; Reece, Sarah E. // Parasites & Vectors;2011, Vol. 4 Issue 1, p44 

    Apoptosis-like programmed cell death (PCD) has recently been described in multiple taxa of unicellular protists, including the protozoan parasites Plasmodium, Trypanosoma and Leishmania. Apoptosis-like PCD in protozoan parasites shares a number of morphological features with programmed cell...

  • INHIBITION OF TOXOPLASMA GONDII AND PLASMODIUM FALCIPARUM INFECTIONS IN VITRO BY NSC3852, A REDOX ACTIVE ANTIPROLIFERATIVE AND TUMOR CELL DIFFERENTIATION AGENT. Strobl, Jeannine S.; Seibert, Christopher W.; Yunbo Li; Nagarkatti, Rana; Mitchell, Sheila M.; Rosypal, Alexa C.; Rathore, Dharmendar; Lindsay, David S. // Journal of Parasitology;Feb2009, Vol. 95 Issue 1, p215 

    We searched the National Cancer Institute (NCI) compound library for structures related to the antitumor quinoline NSC3852 (5-nitroso-8-quinolinol) and used a computer algorithm to predict the antiprotozoan activity for each of 13 structures. Half of these compounds inhibited Toxoplasma gondii...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics