Brief exercise in creases peripheral blood NK cell counts without immediate functional changes, but impairs their responses to ex vivo stimulation

Millard, Anne-Laure; Valli, Piero V.; Stussi, Georg; Mueller, Nicolas J.; Puga Yung, Gisella; Seebach, Jörg D.
May 2013
Frontiers in Immunology;May2013, Vol. 4, p1
Academic Journal
Physical as well as psychological stress increases the number of circulating peripheral blood NK cells. Whereas some studies found a positive correlation between exercise and NK cell counts and cytotoxic activity, others showed that, for example, heavy training leads to a decrease in per cell NK cytotoxicity. Thus, the impact of exercise on NK cell function and eventually on altered immunocompetence remains to be elucidated. Here, we investigated whether a single bout of brief exercise, consisting in running up and down 150 stair-steps, affects the number and function of circulating NK cells. NK cells, obtained from 29 healthy donors, before and immediately after brief exercise, were assessed for numbers, phenotype, IFNγ production, degranulation, cytotoxicity, and in vitro response to stimulation with IL-2, IL-2/IL-12, or TLR2 agonists. Running resulted in a sixfold increase in the number of CD3-/CD56+ NK cells, but decreased the frequency of CD56bright NK cells about twofold. Brief exercise did not significantly interfere with baseline IFNγ secretion or NK cell cytotoxicity. In vitro stimulation with IL-2 and TLR2 agonists (lipoteichoic acid, and synthetic triacylated lipopeptide Pam3CSK4) enhanced IFNγ-secretion, degranulation, and cytotoxicity mediated by NK cells isolated pre-exercise, but had less effect on NK cells isolated following exercise. There were no differences in response to combined IL-2/IL-12 stimulation. In conclusion, having no obvious impact on baseline NK functions, brief exercise might be used as a simple method to significantly increase the number of CD56dim NK cell available for in vitro experiments. Nevertheless, the observed impaired responses to stimulation suggest an alteration of NK cell-mediated immunity by brief exercise which is at least in part explained by a concomitant decrease of the circulating CD56bright NK cell fraction


Related Articles

  • Abnormal Natural Killer Cell Function in Systemic Sclerosis: Altered Cytokine Production and Defective Killing Activity. Horikawa, Mayuka; Hasegawa, Minoru; Komura, Kazuhiro; Hayakawa, Ikuko; Yanaba, Koichi; Matsushita, Takashi; Takehara, Kazuhiko; Sato, Shinichi // Journal of Investigative Dermatology;Oct2005, Vol. 125 Issue 4, p731 

    Natural killer (NK) cells are innate immune effectors that produce various immunoregulatory cytokines. Recent studies have shown that NK cells are involved in the initiation of autoimmunity. In this study, we determined abnormalities of NK cells in systemic sclerosis (SSc), an autoimmune...

  • Developmental pathways that generate natural-killer-cell diversity in mice and humans. Huntington, Nicholas D.; Vosshenrich, Christian A. J.; Di Santo, James P. // Nature Reviews Immunology;Sep2007, Vol. 7 Issue 9, p703 

    Natural killer (NK) cells are large granular lymphocytes capable of producing inflammatory cytokines and spontaneously killing malignant, infected or 'stressed' cells. These NK-cell functions are controlled by cell-surface receptors that titrate stimulatory and inhibitory signals. However, we...

  • Mouse Natural Killer (NK) Cells Express the Nerve Growth Factor Receptor TrkA, which Is Dynamically Regulated. Ralainirina, Natacha; Brons, Nicolaas H. C.; Ammerlaan, Wim; Hoffmann, Céline; Hentges, François; Zimmer, Jacques // PLoS ONE;2010, Vol. 5 Issue 12, p1 

    Background: Nerve growth factor (NGF) is a neurotrophin crucial for the development and survival of neurons. It also acts on cells of the immune system which express the NGF receptors TrkA and p75NTR and can be produced by them. However, mouse NK cells have not yet been studied in this context....

  • 5-Hydroxytryptamine Modulates Migration, Cytokine and Chemokine Release and T-Cell Priming Capacity of Dendritic Cells In Vitro and In Vivo. Müller, Tobias; Dürk, Thorsten; Blumenthal, Britta; Grimm, Melanie; Cicko, Sanja; Panther, Elisabeth; Sorichter, Stephan; Herouy, Yared; Virgilio, Francesco Di; Ferrari, Davide; Norgauer, Johannes; Idzko, Marco // PLoS ONE;2009, Vol. 4 Issue 7, p1 

    Beside its well described role in the central and peripheral nervous system 5-hydroxytryptamine (5-HT), commonly known as serotonin, is also a potent immuno-modulator. Serotoninergic receptors (5-HTR) are expressed by a broad range of inflammatory cell types, including dendritic cells (DCs). In...

  • Natural Killer Cell Function, an Important Target for Infection and Tumor Protection, Is Impaired in Type 2 Diabetes. Berrou, Jeannig; Fougeray, Sophie; Venot, Marion; Chardiny, Victor; Gautier, Jean-François; Dulphy, Nicolas; Toubert, Antoine; Peraldi, Marie-Noëlle // PLoS ONE;Apr2013, Vol. 8 Issue 4, p1 

    :Patients with Type 2 diabetes (T2D) are highly susceptible to infection and have an increased incidence of some tumors, possibly due to immune system dysfunction. In the innate cellular immune system, Natural Killer (NK) lymphocytes are important effectors responsible for controlling infections...

  • Toll-Like Receptor Priming Sensitizes Macrophages to Proinflammatory Cytokine Gene Induction by Deoxynivalenol and Other Toxicants. James Pestka; Hui-Ren Zhou // Toxicological Sciences;Aug2006, Vol. 92 Issue 2, p445 

    Activation of the innate immune system might predispose a host to toxicant-induced inflammation. In vitro macrophage models were employed to investigate the effects of preexposure to Toll-like receptor (TLR) agonists on induction of proinflammatory cytokine gene expression by the trichothecene...

  • IL-23 modulates CD56+/CD3- NK Cell and CD56+/CD3+ NK-like T Cell function differentially from IL-12. Diederik van de Wetering; Roelof A. de Paus; Jaap T. van Dissel; Esther van de Vosse // International Immunology;Feb2009, Vol. 21 Issue 2, p145 

    NK and NK-like T cells play an essential role in linking innate and adaptive immunity through their ability to secrete IFN-γ. The exact trigger initiating production of IFN-γ is uncertain. Antigen-presenting cell (APC)-derived IL-12 is thought to be the classical IFN-γ-inducing cytokine...

  • HIV-1 Nef protein protects infected primary cells against killing by cytotoxic T lymphocytes. Collins, Kathleen L.; Chen, Benjamin K.; Kalams, Spyros A.; Walker, Bruce D.; Baltimore, David // Nature;1/22/1998, Vol. 391 Issue 6665, p397 

    Presents research which examined the ability of cytotoxic T lymphocytes (CTLs) to recognize and kill infected primary T lymphocytes. Role of CTLs; Findings; Resistance of infected cells to CTL killing; How the viral Nef gene protected infected cells; Evasion of CTL lysis by HIV-1-infected cells.

  • Prophylactic IL-12 treatment reduces postoperative metastasis: mediation by increased numbers but not cytotoxicity of NK cells. Schwartz, Yossi; Avraham, Roi; Benish, Marganit; Rosenne, Ella; Ben-Eliyahu, Shamgar // Breast Cancer Research & Treatment;Jan2008, Vol. 107 Issue 2, p211 

    Despite a promising potential, interleukin-12 immunotherapy has yielded limited clinical success while causing perilous toxicities. Here we study a context in which IL-12 may prove clinically beneficial-the removal of the primary tumor, when cell-mediated immunity (CMI) may eradicate minimal...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics