Zebrafish models for ectopic mineralization disorders: practical issues from morpholino design to post-injection observations

Hosen, Mohammad Jakir; Vanakker, Olivier M.; Willaert, Andy; Huysseune, Ann; Coucke, Paul; De Paepe, Anne
May 2013
Frontiers in Genetics;May2013, Vol. 3, p1
Academic Journal
No abstract available.


Related Articles

  • Identification of Sef, a novel modulator of FGF signalling. Tsang, Michael; Friesel, Robert; Kudoh, Tetsuhiro; Dawid, Igor B // Nature Cell Biology;Feb2002, Vol. 4 Issue 2, p165 

    Fibroblast growth factors (FGFs) are members of a family of some 30 secreted proteins important in the regulation of cellular proliferation, migration, differentiation and survival. Here we report the identification of a novel modulator of FGF signal transduction, sef, isolated from a zebrafish...

  • Effective targeted gene ‘knockdown’ in zebrafish. Nasevicius, Aidas; Ekker, Stephen C. // Nature Genetics;Oct2000, Vol. 26 Issue 2, p216 

    The sequencing of the zebrafish genome should be completed by the end of 2002. Direct assignment of function on the basis of this information would be facilitated by the development of a rapid, targeted ‘knockdown’ technology in this model vertebrate. We show here that antisense,...

  • Cascade effect of cardiac myogenesis gene expression during cardiac looping in tbx5 knockdown zebrafish embryos. Jen Her Lu; Jenn Kan Lu; Sie Lin Choo; Yu-Chi Li; Huei-Wen Yeh; Jau-Fen Shiue; Vincent C. Yeh // Journal of Biomedical Science;Nov2008, Vol. 15 Issue 6, p779 

    Zebrafish tbx5 expresses in the heart, pectoral fins and eyes of zebrafish during embryonic development. In zebrafish, injection of tbx5 morpholino antisense RNA caused changes of heart conformation, defect of heart looping, pericardium effusion, dropsy of ventral position and decreased heart...

  • Pituitary adenylate cyclase-activating polypeptide (PACAP) in zebrafish models of nephrotic syndrome. Eneman, Benedicte; Elmonem, Mohamed A.; van den Heuvel, Lambertus P.; Khodaparast, Laleh; Khodaparast, Ladan; van Geet, Chris; Freson, Kathleen; Levtchenko, Elena // PLoS ONE;7/31/2017, Vol. 12 Issue 7, p1 

    Pituitary adenylate cyclase-activating polypeptide (PACAP) is an inhibitor of megakaryopoiesis and platelet function. Recently, PACAP deficiency was observed in children with nephrotic syndrome (NS), associated with increased platelet count and aggregability and increased risk of thrombosis. To...

  • Development of an Automated Imaging Pipeline for the Analysis of the Zebrafish Larval Kidney. Westhoff, Jens H.; Giselbrecht, Stefan; Schmidts, Miriam; Schindler, Sebastian; Beales, Philip L.; Tönshoff, Burkhard; Liebel, Urban; Gehrig, Jochen // PLoS ONE;Dec2013, Vol. 8 Issue 12, p1 

    The analysis of kidney malformation caused by environmental influences during nephrogenesis or by hereditary nephropathies requires animal models allowing the in vivo observation of developmental processes. The zebrafish has emerged as a useful model system for the analysis of vertebrate organ...

  • A PATO-compliant zebrafish screening database (MODB): management of morpholino knockdown screen information. Knowlton, Michelle N.; Tongbin Li; Yongliang Ren; Bill, Brent R.; Ellis, Lynda B. M.; Ekker, Stephen C. // BMC Bioinformatics;2008, Vol. 9, Special section p1 

    Background: The zebrafish is a powerful model vertebrate amenable to high throughput in vivo genetic analyses. Examples include reverse genetic screens using morpholino knockdown, expression-based screening using enhancer trapping and forward genetic screening using transposon insertional...

  • Gas6 Stimulates Angiogenesis of Human Retinal Endothelial Cells and of Zebrafish Embryos via ERK1/2 Signaling. Kim, Young Sook; Jung, Seung-Hyun; Jung, Dong Ho; Choi, So-Jin; Lee, Yu-Ri; Kim, Jin Sook // PLoS ONE;Jan2014, Vol. 9 Issue 1, p1 

    Aim: To determine if growth arrest-specific 6 (Gas6) plays an important role in the regulation of angiogenesis in human retinal microvascular endothelial cells (HRMECs) and in vessel development of zebrafish. Methods: Proliferation, wound-healing cell migration, and tube formation were measured...

  • Loss of Myotubularin Function Results in T-Tubule Disorganization in Zebrafish and Human Myotubular Myopathy. Dowling, James J.; Vreede, Andrew P.; Low, Sean E.; Gibbs, Elizabeth M.; Kuwada, John Y.; Bonnemann, Carsten G.; Feldman, Eva L. // PLoS Genetics;Feb2009, Vol. 5 Issue 2, p1 

    Myotubularin is a lipid phosphatase implicated in endosomal trafficking in vitro, but with an unknown function in vivo. Mutations in myotubularin cause myotubular myopathy, a devastating congenital myopathy with unclear pathogenesis and no current therapies. Myotubular myopathy was the first...

  • Myo1c is an unconventional myosin required for zebrafish glomerular development. Arif, Ehtesham; Kumari, Babita; Wagner, Mark C; Zhou, Weibin; Holzman, Lawrence B; Nihalani, Deepak // Kidney International;Dec2013, Vol. 84 Issue 6, p1154 

    The targeting and organization of podocyte slit diaphragm proteins nephrin and neph1 is critical for development and maintenance of a functional glomerular filtration barrier. Myo1c is a non-muscle myosin motor protein that interacts directly with nephrin and neph1, and mediates their...

  • Neurologic and ocular phenotype in Pitt-Hopkins syndrome and a zebrafish model. Brockschmidt, Antje; Filippi, Alida; Charbel Issa, Peter; Nelles, Michael; Urbach, Horst; Eter, Nicole; Driever, Wolfgang; Weber, Ruthild // Human Genetics;Nov2011, Vol. 130 Issue 5, p645 

    In this study, we performed an in-depth analysis of the neurologic and ophthalmologic phenotype in a patient with Pitt-Hopkins syndrome (PTHS), a disorder characterized by severe mental and motor retardation, carrying a uniallelic TCF4 deletion, and studied a zebrafish model. The PTHS-patient...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics