Optimal choice of functional and off-target effect-reduced siRNAs for RNAi therapeutics

Kumiko Ui-Tei
May 2013
Frontiers in Genetics;May2013, Vol. 3, preceding p1
Academic Journal
No abstract available.


Related Articles

  • RNAi-induced off-target effects in Drosophila melanogaster: frequencies and solutions. Seinen, Erwin; Burgerhof, Johannes G. M.; Jansen, Ritsert C.; Sibon, Ody C. M. // Briefings in Functional Genomics;Jul2011, Vol. 10 Issue 4, p206 

    Genes can be silenced with short-interfering RNA molecules (siRNA). siRNAs are widely used to identify gene functions and have high potential for therapeutic treatments. It is critical that the siRNA specifically targets the expression of the gene of interest but has no off-target effects on...

  • Aptamer-targeted cell-specific RNA interference. Zhou, Jiehua; Rossi, John J. // Silence;2010, Vol. 1 Issue 1, p1 

    This potent ability of small interfering (si)RNAs to inhibit the expression of complementary RNA transcripts is being exploited as a new class of therapeutics for a variety of diseases. However, the efficient and safe delivery of siRNAs into specific cell populations is still the principal...

  • siVirus: web-based antiviral siRNA design software for highly divergent viral sequences. Naito, Yuki; Ui-Tei, Kumiko; Nishikawa, Toru; Takebe, Yutaka; Saigo, Kaoru // Nucleic Acids Research;Jul2006, Vol. 34 Issue 11, pw448 

    siVirus (http://siVirus.RNAi.jp/) is a web-based online software system that provides efficient short interfering RNA (siRNA) design for antiviral RNA interference (RNAi). siVirus searches for functional, off-target minimized siRNAs targeting highly conserved regions of divergent viral...

  • Recent Progress in Chemically Modified siRNAs. Gaglione, M.; Messere, A. // Mini Reviews in Medicinal Chemistry;Jun2010, Vol. 10 Issue 7, p578 

    RNA interference technology has become a powerful laboratory tool to study gene function. Small interfering RNAs (siRNAs) have provided unprecedented opportunities for the development of new therapeutics in human diseases. Unfortunately, siRNA duplexes are not optimal drug-like molecules. The...

  • Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA. Judge, Adam D.; Sood, Vandana; Shaw, Janet R.; Fang, Dianne; McClintock, Kevin; MacLachlan, Ian // Nature Biotechnology;Apr2005, Vol. 23 Issue 4, p457 

    Short interfering RNAs (siRNAs) that mediate specific gene silencing through RNA interference (RNAi) are widely used to study gene function and are also being developed for therapeutic applications. Many nucleic acids, including double- (dsRNA) and single-stranded RNA (ssRNA), can stimulate...

  • Small Interfering RNA in Drug Metabolism and Transport. Ai-Ming Yu // Current Drug Metabolism;Oct2007, Vol. 8 Issue 7, p700 

    RNA interference (RNAi) is a powerful technique that utilizes RNA molecules to specifically knock down the expression of targeted gene at posttranscriptional level. These small interfering RNAs (siRNAs) not only have broad application to basic biomedical research but may be developed as...

  • A simple Bayesian estimate of direct RNAi gene regulation events from differential gene expression profiles. Wilson, Paul A.; Plucinski, Mathew // BMC Genomics;2011, Vol. 12 Issue 1, p250 

    Background: Microarrays are commonly used to investigate both the therapeutic potential and functional effects of RNA interfering (RNAi) oligonucleotides such as microRNA (miRNA) and small interfering RNA (siRNA). However, the resulting datasets are often challenging to interpret as they include...

  • Amides are excellent mimics of phosphate internucleoside linkages and are well tolerated in short interfering RNAs. Mutisya, Daniel; Selvam, Chelliah; Lunstad, Benjamin D.; Pallan, Pradeep S.; Haas, Amanda; Leake, Devin; Egli, Martin; Rozners, Eriks // Nucleic Acids Research;Jun2014, Vol. 42 Issue 10, p6542 

    RNA interference (RNAi) has become an important tool in functional genomics and has an intriguing therapeutic potential. However, the current design of short interfering RNAs (siRNAs) is not optimal for in vivo applications. Non-ionic phosphate backbone modifications may have the potential to...

  • siSPOTR: a tool for designing highly specific and potent siRNAs for human and mouse. Boudreau, Ryan L.; Spengler, Ryan M.; Hylock, Ray H.; Kusenda, Brandyn J.; Davis, Heather A.; Eichmann, David A.; Davidson, Beverly L. // Nucleic Acids Research;Jan2013, Vol. 41 Issue 1, pe9 

    RNA interference (RNAi) serves as a powerful and widely used gene silencing tool for basic biological research and is being developed as a therapeutic avenue to suppress disease-causing genes. However, the specificity and safety of RNAi strategies remains under scrutiny because small inhibitory...

  • Rational Design of Therapeutic siRNAs: Minimizing Off-targeting Potential to Improve the Safety of RNAi Therapy for Huntington's Disease. Boudreau, Ryan L; Spengler, Ryan M; Davidson, Beverly L // Molecular Therapy;Dec2011, Vol. 19 Issue 12, p2169 

    RNA interference (RNAi) provides an approach for the treatment of many human diseases. However, the safety of RNAi-based therapies can be hampered by the ability of small inhibitory RNAs (siRNAs) to bind to unintended mRNAs and reduce their expression, an effect known as off-target gene...


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics