TITLE

Evaluation of γ-oryzanol content and composition from the grains of pigmented rice-germplasms by LC-DAD-ESI/MS

AUTHOR(S)
Heon Woong Kim; Jung Bong Kim; Shanmugavelan, Poovan; Se Na Kim; Young Sook Cho; Haeng Ran Kim; Jeong-Tae Lee; Weon-Tai Jeon; Dong Jin Lee
PUB. DATE
June 2013
SOURCE
BMC Research Notes;2013, Vol. 6 Issue 1, p1
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Background: Rice is the staple food and one of the world's three major grain crops. Rice contains more than 100 bioactive substances including phytic acid, isovitexin, γ-oryzanol, phytosterols, octacosanol, squalene, γ-aminobutyric acid (GABA), tocopherol, tocotrienol derivatives, etc. Out of them, γ-oryzanol is known to have important biological profile such as anti-oxidants, inhibitor of cholesterol oxidation, reduce serum cholesterol levels in animals, effective in the treatment of inflammatory diseases, inhibit tumor growth, reduce blood pressure and promotes food storage stability when used as a food additive, etc. Hence in the present investigation, we aimed to evaluate the content and composition of γ-oryzanol from pigmented rice germplasms using a liquid chromatography with diode array detection and electrospray ionization-mass spectrometry (LC-DAD-ESI/MS). Findings: In the present study, 33 exotic pigmented rice accessions (red, white and purple) have been evaluated. Among them, the contents of γ-oryzanol varied from 3.5 to 21.0 mg/100 g with a mean of 11.2 mg/100 g. A total of ten components of γ-oryzanol including ▵ 7-stigmastenyl ferulate were identified of which, cycloartenyl ferulate, 24-methylenecycloartanyl ferulate, campesteryl ferulate and sitosteryl ferulate were identified as the major components. The mean proportions of steryl ferulates were in the descending order of 24-methylenecycloartanyl ferulate > cycloartenyl ferulate > campesteryl ferulate > sitosteryl ferulate > ▵ 7-campestenyl ferulate > campestanyl ferulate > sitostanyl ferulate > ▵ 7-stigmastenyl ferulate > stigamsteryl ferulate > ▵ 7-sitostenyl ferulate. Almost 11 accessions (33%) showed higher content than the control rice Chucheongbyeo and higher proportions ranged from 10 to 15 mg/100 g. Interestingly, the red rice accession Liberian Coll. B11/B-11 (21.0 mg/100 g) showed higher content γ-oryzanol than control rice Jeokjinjubyeo (19.1 mg/100 g) and the purple rice accession Padi Adong Dumarat, Mardi No.4376 (20.3 mg/100 g) showed a similar content with control rice Heugjinjubyeo (21.4 mg/100 g). Conclusions: Most of analyzed rice accessions were found to possess higher contents of γ-oryzanol than the control rice, Chucheongbyeo. In particular, the red accessions showed highest content than the white and purple accessions. The content and composition of γ-oryzanol in 33 exotic pigmented rice accessions have been evaluated and compared significantly by the present investigation.
ACCESSION #
88014062

 

Related Articles

  • Structural determination of two lipids isolated from the sea urchin Echinometra lucunter. Kaihil, A.; Diop, M.; Samb, A. // Chemistry of Natural Compounds;Jan2012, Vol. 47 Issue 6, p932 

    Specimens of the sea urchin Echinometra lucunter were collected at Dakar (Senegal). Fresh sea urchins were exhaustively treated with CHCl-MeOH 1:1 (v/v). After evaporation of solvent the oily residue (1, 92 g) was passed through an RP 18 column. The CHCl soluble fraction has been analyzed...

  • Derivatization for Ultratrace Quantitative Analysis Using HPLC-ESI-MS. Schug, Kevin A. // LC-GC North America;Apr2013, Vol. 31 Issue 4, p334 

    A blog related to the derivatization technique electrospray ionization (ESI) is presented.

  • Erratum to: Mechanical ion gate for electrospray-ionization ion-mobility spectrometry. Li Zhou; Collins, David C.; Lee, Edgar D.; Rockwood, Alan L.; Lee, Milton L. // Analytical & Bioanalytical Chemistry;Oct2010, Vol. 398 Issue 4, p1833 

    A correction to the article "Mechanical Ion Gate for Electrospray-Ionization Ion-Mobility Spectrometry" that was published in the online version of the journal on August 14, 2010 issue is presented.

  • Mass analysis of receptor?DNA complexes. DeWitt, Natalie // Nature Biotechnology;Dec99, Vol. 17 Issue 12, p1150 

    Focuses on the use of electrospray ionization mass spectrometry (ESI-MS) in the study of protein complexes. Use of ESI-MS in the analysis of the effect of ligands on heterodimerization of full-length vitamin D and retinoid X receptors.

  • Development of a novel method for quantification of sterols and oxysterols by UPLC-ESI-HRMS: application to a neuroinflammation rat model. Ayciriex, Sophie; Regazzetti, Anne; Gaudin, Mathieu; Prost, Elise; Dargère, Delphine; Massicot, France; Auzeil, Nicolas; Laprévote, Olivier // Analytical & Bioanalytical Chemistry;Dec2012, Vol. 404 Issue 10, p3049 

    Cholesterol and oxysterols are involved as key compounds in the development of severe neurodegenerative diseases and in neuroinflammation processes. Monitoring their concentration changes under pathological conditions is of interest to get insights into the role of lipids in diseases. For...

  • Ion Traps Provide High-Throughput Analysis. Stafford, George // R&D Magazine;Nov2001, Vol. 43 Issue 11, p49 

    Presents information on the unique properties of quadrupole ion trap mass spectrometry (MS) that make it a versatile technology for researchers in both chemistry and biology. Features of the ion trap MS device called LCQ Deca XP from Thermo Finnigan company; Difference of quadrupoles from ions...

  • The fractionation/concentration of aquatic humic substances by the sequential membrane system and their characterization with mass spectrometry. Ikeda, K.; Arimura, R.; Echigo, S.; Shimizu, Y.; Minear, R.A.; Matsui, S. // Water Science & Technology;2000, Vol. 42 Issue 7/8, p383 

    Reports on the fractionation/concentration of aquatic humic substances by the sequential membrane system and their characterization with mass spectrometry. Mass spectra of the Suwannee River Fulvic Acid (SRFA); Sequential membrane system; Characterization of the fractions obtained by the...

  • Comparison of Dissociation of Ions in an Electrospray Source, or a Collision Cell in Tandem Mass Spectrometry. Bure, Corinne; Lange, Catherine // Current Organic Chemistry;Oct2003, Vol. 7 Issue 15, p1613 

    Electrospray ionization (ESI) is a soft ionization technique well suited for producing gas phase ions of biological macromolecules. The use of ESI when combined with collision-induced dissociation and mass analysis can give structural information about biomolecules. Traditionally, collisional...

  • Electrospray ionization innovation displayed at PITTCON.  // R&D Magazine;Apr2003, Vol. 45 Issue 4, p8 

    Focuses on the ColdSpray electrospray ionization source developed by JEOL USA. Structures that can be studied using the ColdSpray.

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics