Effect of Two Fungicides (Caocobre and Ridomil) on Germination and Radicle Elongation of Cocoa (Theobroma Cacao L) Seedlings

Minamor, Andrew Amegbedzi
March 2013
International Journal of Pure & Applied Sciences & Technology;Mar2013, Vol. 15 Issue 1, p79
Academic Journal
Studies on the effect of two fungicides Caocobre Sandoz and Ridomil plus 72 on the vegetative growth and radicle elongation of cocoa seedlings were investigated using the Blotter Test Method. The germination media (fungicides) were of varied concentration; undiluted, (1:1, 1:5, 1: 10) v/v. Control samples did not receive fungicide treatment. Five (5) cocoa beans with testa removed to quicken germination were placed in sterile filter paper in 12.5 cm diameter Petri dishes moistened with 10ml of fungicides of appropriate dilution. There were three (3) replicates for each treatment. The Petri dishes were then incubated in darkness for seven days. Both fungicides severely depressed radicle length of cocoa beans. The effect was more pronounced at the higher concentration but this depression in length was decreased with dilution of the fungicides. Both Caocobre and Ridomil at all dilution used permitted the formation of lateral roots by cocoa seedlings. However, the emerging roots eventually died in plates containing high concentration dilution of the fungicides. The fungicides depressed dry matter accumulation of leaves. The final dry weight of leaves after 70 days in pots treated with Caocobre was 8.2 x 103mg; leaf of plants treated with Ridomil weighed 6.3 x 103 mg as compared to 10.4 x 103 mg weight of leaves in the control. There was however, no statistical differences (P = 0.05 student t - test) between the treatments and the control when dry weight of stem and roots were compared. The heights of the seedlings treated with the fungicides Caocobre and Ridomil approximated that of the control throughout the growing period.


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