TITLE

Dual-Specific Interaction to Detect DNA on Gold Nanoparticles

AUTHOR(S)
Feng, Chuan-Liang; Dou, Xiao-Qiu; Liu, Qing-Lei; Wang Zhang; Gu, Jia-Jun; Zhu, Shen-Min; Aveling Jenkins, Andrew Tobias; Di Zhang
PUB. DATE
May 2013
SOURCE
Sensors (14248220);May2013, Vol. 13 Issue 5, p5749
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
An approach to selectively and efficiently detect single strand DNA is developed by using streptavidin coated gold nanoparticles (StAuNPs) as efficient quenchers. The central concept for the successful detection is the combination the of streptavidin-biotin interaction with specific probe-target DNA hybridization. Biotin labeled probe DNAs act as "bridges" to bring Cy5 labeled targets to the particle surface and the fluorophore dye can be rapidly and efficiently quenched by StAuPNs. By measuring the changes of photoluminescence intensity of Cy5, an efficient, selective, and reversed detection of DNA hybridization is realized. The methodology may pave a new way for simple and rapid detections of biomolecules.
ACCESSION #
87738977

 

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