Decreased expression levels of PRM1 and PRM2 genes in testes of infertile men referred to Royan Institute

Eelaminejad, Z.; Heaydaryan, N.; Favaedi, R.; Sadighi Gilani, M. A.; Shahhoseini, M.
April 2013
Iranian Journal of Reproductive Medicine;AprSupp, p39
Academic Journal
Introduction: Spermatogenesis is a complex and unique process in male reproductive system which transforms diploid spermatogonia into differentiated haploid spermatozoa. The final stage of this process needs extensive chromatin condensation. This condensation occurs by replacement of histones by transition proteins which are subsequently replaced by protamines. Materials and Methods: Protamines are the major nuclear sperm proteins that two types of them are known as protamine 1 (PRM1) and protamine 2 (PRM2). Several functional studies have been reported for these proteins through spermatogenesis, such as: condensation of the sperm nucleus, protection of the paternal genetic message delivered by the spermatozoa and involvement in the epigenetic imprinting of the paternal genome. In this study the expression profiles of PRM1 and PRM2 genes were evaluated in testis tissues of infertile men. Ethical approval and informed patient consent was gained for the use of tissue samples. Testicular biopsies were collected from 20 infertile men referred to Royan Institute and underwent testicular sperm extraction (TESE). Through pathological and spermogram analyses, these samples distributed into 4 groups: Hypospermatogenesis (positive control), Sever oligoasthenoteratozoospermia, complete maturation arrest at spermatide level and Sertoli cell only syndrome (negative control). Each group contained 5 samples. Results: Total RNA was extracted from the tissue samples. After synthesis of first-strand cDNA, quantitative real-time PCR was performed using designed PRM1 and PRM2 primers. qRT-PCR analysis significantly revealed lower expression of PRM1, PRM2 in all groups compared to positive control. Conclusion: Our finding supported a correlation between men infertility and protamine deficiency in infertile men referred to Royan Institute.


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