TITLE

A Comparison of the Olfactory Gene Repertoires of Adults and Larvae in the Noctuid Moth Spodoptera littoralis

AUTHOR(S)
Poivet, Erwan; Gallot, Aurore; Montagné, Nicolas; Glaser, Nicolas; Legeai, Fabrice; Jacquin-Joly, Emmanuelle
PUB. DATE
April 2013
SOURCE
PLoS ONE;Apr2013, Vol. 8 Issue 4, p1
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
To better understand the olfactory mechanisms in a lepidopteran pest model species, the cotton leafworm Spodoptera littoralis, we have recently established a partial transcriptome from adult antennae. Here, we completed this transcriptome using next generation sequencing technologies, namely 454 and Illumina, on both adult antennae and larval tissues, including caterpillar antennae and maxillary palps. All sequences were assembled in 77,643 contigs. Their analysis greatly enriched the repertoire of chemosensory genes in this species, with a total of 57 candidate odorant-binding and chemosensory proteins, 47 olfactory receptors, 6 gustatory receptors and 17 ionotropic receptors. Using RT-PCR, we conducted the first exhaustive comparison of olfactory gene expression between larvae and adults in a lepidopteran species. All the 127 candidate olfactory genes were profiled for expression in male and female adult antennae and in caterpillar antennae and maxillary palps. We found that caterpillars expressed a smaller set of olfactory genes than adults, with a large overlap between these two developmental stages. Two binding proteins appeared to be larvae-specific and two others were adult-specific. Interestingly, comparison between caterpillar antennae and maxillary palps revealed numerous organ-specific transcripts, suggesting the complementary involvement of these two organs in larval chemosensory detection. Adult males and females shared the same set of olfactory transcripts, except two male-specific candidate pheromone receptors, two male-specific and two female-specific odorant-binding proteins. This study identified transcripts that may be important for sex-specific or developmental stage-specific chemosensory behaviors.
ACCESSION #
87677334

 

Related Articles

  • Regulation of c-Fos gene expression in the rat olfactory bulb during olfactory learning. N. Solov’eva; L. Lagutina; L. Antonova; K. Anokhin // Neuroscience & Behavioral Physiology;Sep2007, Vol. 37 Issue 7, p697 

    Abstract  An immunohistochemical method was used to study the expression of transcription factor c-Fos in the mid part of the main olfactory bulbs in 18-day-old rats after training to seek their mothers using an olfactory orientation cue (propionic acid) in a Y-maze. On the next day, rat...

  • Catalogue of epidermal genes: Genes expressed in the epidermis during larval molt of the silkworm Bombyx mori. Okamoto, Shun; Futahashi, Ryo; Kojima, Tetsuya; Mita, Kazuei; Fujiwara, Haruhiko // BMC Genomics;2008, Vol. 9, Special section p1 

    Background: The insect cuticle is composed of various proteins and formed during the molt under hormonal regulation, although its precise composition and formation mechanism are largely unknown. The exhaustive catalogue of genes expressed in epidermis at the molt constitutes a massive amount of...

  • A Core Filamentation Response Network in Candida albicans Is Restricted to Eight Genes. Martin, Ronny; Albrecht-Eckardt, Daniela; Brunke, Sascha; Hube, Bernhard; Hünniger, Kerstin; Kurzai, Oliver // PLoS ONE;Mar2013, Vol. 8 Issue 3, p1 

    Although morphological plasticity is a central virulence trait of Candida albicans, the number of filament-associated genes and the interplay of mechanisms regulating their expression remain unknown. By correlation-based network modeling of the transcriptional response to different defined...

  • TTF1 expression in normal lung neuroendocrine cells and related tumors: immunohistochemical study comparing two different monoclonal antibodies. La Rosa, Stefano; Chiaravalli, Anna; Placidi, Claudia; Papanikolaou, Nikolaos; Cerati, Michele; Capella, Carlo; Chiaravalli, Anna Maria // Virchows Archiv;Oct2010, Vol. 457 Issue 4, p497 

    Thyroid transcription factor-1 (TTF1) regulates lung morphogenesis and differentiation, and its immunohistochemical expression is used for identifying lung neoplasms. The 8G7G3/1 antibody has been used in previous studies, but a different and more sensitive anti-TTF1 antibody, named SPT24, has...

  • Activating transcription factor 5 (ATF5) is essential for the maturation and survival of mouse basal vomeronasal sensory neurons. Nakano, Haruo; Iida, Yoshitaka; Suzuki, Makoto; Aoki, Marie; Umemura, Mariko; Takahashi, Shigeru; Takahashi, Yuji // Cell & Tissue Research;Mar2016, Vol. 363 Issue 3, p621 

    Activating transcription factor 5 (ATF5) is a member of the CREB/ATF family of transcription factors, which is highly expressed in olfactory chemosensory tissues, the main olfactory epithelium and vomeronasal epithelium (VNE) in mice. The vomeronasal sensory neurons in the VNE detect pheromones...

  • The Unc-5 Receptor Is Directly Regulated by Tinman in the Developing Drosophila Dorsal Vessel. Asadzadeh, Jamshid; Neligan, Niamh; Canabal-Alvear, Judith J.; Daly, Amanda C.; Kramer, Sunita Gupta; Labrador, Juan-Pablo // PLoS ONE;9/10/2015, Vol. 10 Issue 9, p1 

    During early heart morphogenesis cardiac cells migrate in two bilateral opposing rows, meet at the dorsal midline and fuse to form a hollow tube known as the primary heart field in vertebrates or dorsal vessel (DV) in Drosophila. Guidance receptors are thought to mediate this evolutionarily...

  • Effects of an Artificial Smoke on Arousals During Human Sleep. Heiser, C.; Baja, J.; Lenz, F.; Sommer, J.; Hörmann, K.; Herr, R.; Stuck, B. // Chemosensory Perception;Dec2012, Vol. 5 Issue 3/4, p274 

    The central processing of olfactory stimuli is different compared to other sensory systems, as they are not followed by arousals at least with regard to pure olfactory substances such as hydrogen sulfite. It is still unclear, however, whether a stimulus with a higher significance to the sleeper...

  • Comparative transcriptome profiling of the fertile and sterile flower buds of a dominant genic male sterile line in sesame (Sesamum indicum L.). Hongyan Liu; Mingpu Tan; Haijuan Yu; Liang Li; Fang Zhou; Minmin Yang; Ting Zhou; Yingzhong Zhao // BMC Plant Biology;11/10/2016, Vol. 16, p1 

    Background: Sesame (Sesamum indicum L.) is a globally important oilseed crop with highly-valued oil. Strong hybrid vigor is frequently observed within this crop, which can be exploited by the means of genic male sterility (GMS). We have previously developed a dominant GMS (DGMS) line W1098A that...

  • NOTICIAS GENERALES.  // SHILAP Revista de Lepidopterologia;Jun2011, Vol. 39 Issue 154, p240 

    No abstract available.

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics