TITLE

Microplate-compatible total internal reflection fluorescence microscopy for receptor pharmacology

AUTHOR(S)
Chen, Minghan; Zaytseva, Natalya V.; Wu, Qi; Li, Min; Fang, Ye
PUB. DATE
May 2013
SOURCE
Applied Physics Letters;5/13/2013, Vol. 102 Issue 19, p193702
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
We report the use of total internal reflection fluorescence (TIRF) microscopy for analyzing receptor pharmacology and the development of a microplate-compatible TIRF imaging system. Using stably expressed green fluorescence protein tagged β2-adrenergic receptor as the reporter, we found that the activation of different receptors results in distinct kinetic signatures of the TIRF intensity of cells. These TIRF signatures closely resemble the characteristics of their respective label-free dynamic mass redistribution signals in the same cells. This suggests that TIRF in microplate can be used for profiling and screening drugs.
ACCESSION #
87655996

 

Related Articles

  • Microscopy's Expanding Role. Karet, Gail; Studt, Tim // R&D Magazine;Aug2000, Vol. 42 Issue 8, p16 

    Discusses the impact of technology on the field of optical microscopy. Major technological changes in microscopy; Information on the optical technique called total internal reflection fluorescence microscopy; Recommendations for buying a microscope.

  • BLAZING FUTURE Paths in TIRF. Semiatin, Ilene K. // R&D Magazine;Aug2006, Vol. 48 Issue 8, p24 

    The article reports on the developments in total internal reflection fluorescence (TIRF) microscopy, a method for viewing single molecule and cellular fluorescence at locations where molecules interact with surfaces. A development has been the introduction of high-quality chromatic correction....

  • Variable-angle total internal reflection fluorescence microscopy of intact cells of Arabidopsis thaliana.  // Plant Methods;2011, Vol. 7 Issue 1, p27 

    The article cites a research study that uses a new technique known as variable-angle epifluorescence microscopy (VAEM) as tool for observing fluorescently labeled molecules on the plasma membrane surface of animal cells. However, as plants have cell walls, thick peripheral layers surrounding the...

  • Rab10 delivers GLUT4 storage vesicles to the plasma membrane. Yu Chen; Lippincott-Schwartz, Jennifer // Communicative & Integrative Biology;May/Jun2013, Vol. 6 Issue 3, pe23779-1 

    The article offers an assessment on the specific roles played by different Rab proteins in trafficking the glucose transporter GLUT4 by performing advanced live cell imaging using total internal reflection fluorescence (TIRF) microscopy. It was found that Rab14 labels transferin-positive,...

  • Detection of single-molecule DNA hybridization by using dual-color total internal reflection fluorescence microscopy. Kang, Seong Ho; Kim, Yun-Jeong; Yeung, Edward S. // Analytical & Bioanalytical Chemistry;Apr2007, Vol. 387 Issue 8, p2663 

    We examined the use of prism-type simultaneous dual-color total internal reflection fluorescence microscopy (TIRFM) to probe DNA molecules at the single-molecule level. The system allowed the direct detection of the complementary interactions between single-stranded probe DNA molecules (16-mer)...

  • Transparent Electrode Materials for Simultaneous Amperometric Detection of Exocytosis and Fluorescence Microscopy. Kisler, Kassandra; Kim, Brian N.; Xin Liu; Berberian, Khajak; Qinghua Fang; Mathai, Cherian J.; Gangopadhyay, Shubhra; Gillis, Kevin D.; Lindau, Manfred // Journal of Biomaterials & Nanobiotechnology;May2012 Special Issue, Vol. 3, p243 

    We have developed and tested transparent microelectrode arrays capable of simultaneous amperometric measurement of oxidizable molecules and fluorescence imaging through the electrodes. Surface patterned microelectrodes were fabricated from three different conducting materials: Indium-tin-oxide...

  • Probing the precision of the mitotic clock with a live-cell fluorescent biosensor. Meyer, Tobias; Jones, Joshua T.; Myers, Jason W.; Ferrell, Jr, James E. // Nature Biotechnology;Mar2004, Vol. 22 Issue 3, p306 

    Precise timing of mitosis is essential for high-fidelity cell duplication. However, temporal measurements of the mitotic clock have been challenging. Here we present a fluorescent mitosis biosensor that monitors the time between nuclear envelope breakdown (NEB) and re-formation using parallel...

  • Compact diode-side-pumped Nd:YLF laser with high beam quality. Sousa, Eduardo Colombo; Raniere, Izilda Márcia; Baldochi, Sonia Lícia; Wetter, Niklaus Ursus // AIP Conference Proceedings;4/15/2008, Vol. 992 Issue 1, p426 

    We describe the performance of a diode-side-pumped Nd:YLF laser using a compact cavity based on total internal reflection inside the gain media, showing the beam quality improvement with multiple pass configuration. With one pass through the crystal, high efficiency in multimode operation with 6...

  • Injection-seeded internal-reflection-mode p-Ge laser exceeds 10 W peak terahertz power. Muravjov, A. V.; Saxena, H.; Peale, R. E.; Fredricksen, C. J.; Edwards, O.; Shastin, V. N. // Journal of Applied Physics;Apr2008, Vol. 103 Issue 8, p083112 

    Injection seeding of a large active p-Ge laser crystal operating on total internal reflection modes is demonstrated with peak output power at the level of 40 W in the 1.5–4.2 THz spectral range. The improvement over traditional 1 W axial mode p-Ge lasers is due both to spatially and...

Share

Read the Article

Courtesy of THE LIBRARY OF VIRGINIA

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics