TITLE

Intracellular temperature mapping with fluorescence-assisted photoacoustic-thermometry

AUTHOR(S)
Gao, Liang; Zhang, Chi; Li, Chiye; Wang, Lihong V.
PUB. DATE
May 2013
SOURCE
Applied Physics Letters;5/13/2013, Vol. 102 Issue 19, p193705
SOURCE TYPE
Academic Journal
DOC. TYPE
Article
ABSTRACT
Measuring intracellular temperature is critical to understanding many cellular functions but still remains challenging. Here, we present a technique-fluorescence-assisted photoacoustic thermometry (FAPT)-for intracellular temperature mapping applications. To demonstrate FAPT, we monitored the intracellular temperature distribution of HeLa cells with sub-degree (0.7 °C) temperature resolution and sub-micron (0.23 μm) spatial resolution at a sampling rate of 1 kHz. Compared to traditional fluorescence-based methods, FAPT features the unique capability of transforming a regular fluorescence probe into a concentration- and excitation-independent temperature sensor, bringing a large collection of commercially available generic fluorescent probes into the realm of intracellular temperature sensing.
ACCESSION #
87655939

 

Related Articles

Share

Read the Article

Courtesy of VIRGINIA BEACH PUBLIC LIBRARY AND SYSTEM

Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics