Tracking Dynamic Microvascular Changes during Healing after Complete Biopsy Punch on the Mouse Pinna Using Optical Microangiography

Jung, Yeongri; Dziennis, Suzan; Zhi, Zhongwei; Reif, Roberto; Zheng, Ying; Wang, Ruikang K.
February 2013
PLoS ONE;Feb2013, Vol. 8 Issue 2, p1
Academic Journal
Optical microangiography (OMAG) and Doppler optical microangiography (DOMAG) are two non-invasive techniques capable of determining the tissue microstructural content, microvasculature angiography, and blood flow velocity and direction. These techniques were used to visualize the acute and chronic microvascular and tissue responses upon an injury in vivo. A tissue wound was induced using a 0.5 mm biopsy punch on a mouse pinna. The changes in the microangiography, blood flow velocity and direction were quantified for the acute (<30 min) wound response and the changes in the tissue structure and microangiography were determined for the chronic wound response (30 min–60 days). The initial wound triggered recruitment of peripheral capillaries, as well as redirection of main arterial and venous blood flow within 3 min. The complex vascular networks and new vessel formation were quantified during the chronic response using fractal dimension. The highest rate of wound closure occurred between days 8 and 22. The vessel tortuosity increased during this time suggesting angiogenesis. Taken together, these data signify that OMAG has the capability to track acute and chronic changes in blood flow, microangiography and structure during wound healing. The use of OMAG has great potential to improve our understanding of vascular and tissue responses to injury in order to develop more effective therapeutics.


Related Articles

  • The C57BL/6J Mouse Exhibits Sporadic Congenital Portosystemic Shunts. Cudalbu, Cristina; McLin, Valérie A.; Lei, Hongxia; Duarte, Joao M. N.; Rougemont, Anne-Laure; Oldani, Graziano; Terraz, Sylvain; Toso, Christian; Gruetter, Rolf // PLoS ONE;Jul2013, Vol. 8 Issue 7, p1 

    C57BL/6 mice are the most widely used strain of laboratory mice. Using in vivo proton Magnetic Resonance Spectroscopy (1H MRS), we have repeatedly observed an abnormal neurochemical profile in the brains of both wild-type and genetically modified mice derived from the C57BL/6J strain, consisting...

  • Alteration of angiogenic patterns on B16BL6 melanoma development promoted in Matrigel. Kitahara, Shuji; Morikawa, Shunichi; Shimizu, Kazuhiko; Abe, Hiroyuki; Ezaki, Taichi // Medical Molecular Morphology;Mar2010, Vol. 43 Issue 1, p26 

    Because the progression and metastasis of solid tumors depend on their local microcirculation, we sought to characterize tumor angiogenesis three dimensionally in a highly metastatic mouse melanoma model, B16BL6 (B16), injected with Matrigel into the subcutis in the skin on the back of syngeneic...

  • Thiram inhibits angiogenesis and slows the development of experimental tumours in mice. Marikovsky, M. // British Journal of Cancer;3/4/2002, Vol. 86 Issue 5, p779 

    Thiram-tetramethylthiuram disulphide--a chelator of heavy metals, inhibited DNA synthesis and induced apoptosis in cultured bovine capillary endothelial cells. Bovine capillary endothelial cells were 10-60-fold more sensitive to thiram than other cell types. These effects were prevented by...

  • Bone Marrow-Derived Progenitor Cells Augment Venous Remodeling in a Mouse Dorsal Skinfold Chamber Model. Doyle, Megan E.; Perley, Jeffrey P.; Skalak, Thomas C. // PLoS ONE;Feb2012, Vol. 7 Issue 2, p1 

    The delivery of bone marrow-derived cells (BMDCs) has been widely used to stimulate angiogenesis and arteriogenesis. We identified a progenitor-enriched subpopulation of BMDCs that is able to augment venular remodeling, a generally unexplored area in microvascular research. Two populations of...

  • Corrigendum: Comparison of stimulus-evoked cerebral hemodynamics in the awake mouse and under a novel anesthetic regime.  // Scientific Reports;10/9/2015, p1 

    A correction to the article "Comparison of stimulus-evoked cerebral hemodynamics in the awake mouse and under a novel anesthetic regime" that was published online in the July 28, 2015 issue is presented.

  • In-vivo and ex-vivo characterization of laser-induced choroidal neovascularization variability in mice. Hoerster, Robert; Muether, Philipp; Vierkotten, Sarah; Schröder, Susanne; Kirchhof, Bernd; Fauser, Sascha // Graefe's Archive of Clinical & Experimental Ophthalmology;Nov2012, Vol. 250 Issue 11, p1579 

    Background: Retinal argon laser coagulation is an established procedure for induction of choroidal neovascularization (CNV) in rodents. This study aimed to evaluate the in-vivo and ex-vivo morphology and variability of laser-induced CNV spots over time. Methods: Female C57/Bl/6 mice, 3-6 months...

  • Tenomodulin Inhibits Retinal Neovascularization in a Mouse Model of Oxygen-Induced Retinopathy. Wei Wang; Zhongqiu Li; Sato, Tatsuhiko; Oshima, Yusuke // International Journal of Molecular Sciences;Nov2012, Vol. 13 Issue 11, p15373 

    We aimed to determine the anti-angiogenic effect of tenomodulin (TeM) on retinal neovascularization in an oxygen-induced retinopathy (OIR) mouse model. OIR was induced in C57BL/6 mice by exposing seven-day-old mice to 75% oxygen for five days followed by room air for five days. Control mice were...

  • Antiangiogenic Effects of Doxazosin on Experimental Choroidal Neovascularization in Mice. Guo, Jiaxian; Luo, Xueting; Liang, Jian; Xiao, Meichun; Sun, Xiaodong // Journal of Ocular Pharmacology & Therapeutics;Jan/Feb2017, Vol. 33 Issue 1, p50 

    Purpose: The present study was designed to evaluate the effects of doxazosin on experimental choroidal neovascularization (CNV) in mice.Methods: Six- to 8-week-old male C57BL/6 mice were divided into a control group and a doxazosin-treated group (5 mg/kg, i.p.,...

  • The effects of cashew gum as anti-hypertensive agent. Carestiato, Tatiana; Aguila, Marcia B.; Mothé, Cheila G. // Journal of Thermal Analysis & Calorimetry;Aug2009, Vol. 97 Issue 2, p717 

    The effects of cashew gum over heart samples of spontaneously hypertensive rats (SHR) were studied by Thermal Analysis and Micrographs. During the experiment, ten rats received daily by gavage 500 mg/kg of cashew gum (CG groups) and the other ten rats received only fresh water (Control groups)....


Read the Article


Sorry, but this item is not currently available from your library.

Try another library?
Sign out of this library

Other Topics